EXPRESSION OF MESSENGER RNA SPLICE VARIANTS FOR VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) IN THE ADULT RAT AND HUMAN PENIS

Abstract Hypoxia is a strong stimulus for the transcription of a set of genes, including erythropoietin and vascular endothelial growth factor. Here we report on the cloning, functional significance, and expression of a complementary DNA (cDNA) that is involved in hypoxia-mediated expression of these 2 genes. The full-length cDNA encodes a predicted protein of 806 amino acids that contains a leucine zipper motif. This protein, termed HAF for hypoxia-associated factor, binds to a 17-base pair (bp) region of the erythropoietin promoter, which was shown earlier to participate in hypoxia-induced expression of the erythropoietin gene. In Hep3B cells, clones modified to express HAF antisense RNA showed an attenuated response to hypoxia-mediated induction of both erythropoietin and vascular endothelial growth factor transcription. HAF showed sequence-specific interaction with a DNA element in the 5′ untranslated region ofVEGF gene. The HAF 2.6-kilobase (kb) messenger RNA (mRNA) is expressed in most adult tissues. The highest expression occurs in fetal liver and the least in adult liver. HAF is the murine homolog of Sart-1, a 125-kd human protein expressed in the nuclei of normal and malignant cells.

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Extraocular motoneurons of the adult rat show higher levels of vascular endothelial growth factor and its receptor Flk-1 than other cranial motoneurons

PLoS ONE ◽

10.1371/journal.pone.0178616 ◽

2017 ◽

Vol 12 (6) ◽

pp. e0178616 ◽

Cited By ~ 6

Author(s):

Silvia Silva-Hucha ◽

Rosendo G. Hernández ◽

Beatriz Benítez-Temiño ◽

Ángel M. Pastor ◽

Rosa R. de la Cruz ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Vascular Endothelial ◽

Adult Rat ◽

Extraocular Motoneurons ◽

Endothelial Growth Factor

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The splice variants VEGF121 and VEGF189 of the angiogenic peptide vascular endothelial growth factor are expressed in osteoarthritic cartilage

Arthritis & Rheumatism ◽

10.1002/1529-0131(200105)44:5<1082::aid-anr188>3.0.co;2-x ◽

2001 ◽

Vol 44 (5) ◽

pp. 1082-1088 ◽

Cited By ~ 109

Author(s):

Thomas Pufe ◽

Wolf Petersen ◽

Bernhard Tillmann ◽

Rolf Mentlein

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Splice Variants ◽

Vascular Endothelial ◽

Osteoarthritic Cartilage ◽

Endothelial Growth Factor

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A new transacting factor that modulates hypoxia-induced expression of the erythropoietin gene

Blood ◽

10.1182/blood.v96.2.491.014k28_491_497 ◽

2000 ◽

Vol 96 (2) ◽

pp. 491-497 ◽

Cited By ~ 1

Author(s):

Madhu Gupta ◽

Paul T. Mungai ◽

Eugene Goldwasser

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Messenger Rna ◽

Leucine Zipper ◽

Fetal Liver ◽

Specific Interaction ◽

Vascular Endothelial ◽

Induced Expression ◽

Murine Homolog ◽

Endothelial Growth Factor

Hypoxia is a strong stimulus for the transcription of a set of genes, including erythropoietin and vascular endothelial growth factor. Here we report on the cloning, functional significance, and expression of a complementary DNA (cDNA) that is involved in hypoxia-mediated expression of these 2 genes. The full-length cDNA encodes a predicted protein of 806 amino acids that contains a leucine zipper motif. This protein, termed HAF for hypoxia-associated factor, binds to a 17-base pair (bp) region of the erythropoietin promoter, which was shown earlier to participate in hypoxia-induced expression of the erythropoietin gene. In Hep3B cells, clones modified to express HAF antisense RNA showed an attenuated response to hypoxia-mediated induction of both erythropoietin and vascular endothelial growth factor transcription. HAF showed sequence-specific interaction with a DNA element in the 5′ untranslated region ofVEGF gene. The HAF 2.6-kilobase (kb) messenger RNA (mRNA) is expressed in most adult tissues. The highest expression occurs in fetal liver and the least in adult liver. HAF is the murine homolog of Sart-1, a 125-kd human protein expressed in the nuclei of normal and malignant cells.

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