Significance of Elevated Thrombin-Antithrombin III Complex and Plasmin-??2-Plasmin Inhibitor Complex in the Acute Stage of Nontraumatic Subarachnoid Hemorrhage

Neurosurgery ◽  
1994 ◽  
Vol 35 (6) ◽  
pp. 1055???1060 ◽  
Author(s):  
Youichi Itoyama ◽  
Shodo Fujioka ◽  
Shuichi Takaki ◽  
Motohiro Morioka ◽  
Takuichiro Hide ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Antithrombin Iii ◽  
Acute Stage ◽  
Inhibitor Complex ◽  
Thrombin Antithrombin Iii Complex ◽  
Plasmin Inhibitor

scholarly journals Thrombin-antithrombin III Complex and Plasmin-α2 plasmin inhibitor Complex during Pregnancy

1993 ◽  
Vol 4 (2) ◽  
pp. 116-120
Author(s):  
Hiroo ONDA ◽  
Akiteru TOKUNAGA ◽  
Hiroshi TOMA ◽  
Masayoshi SANADA ◽  
Kazue TAKAI
Keyword(s):  
Antithrombin Iii ◽  
Inhibitor Complex ◽  
Thrombin Antithrombin Iii Complex ◽  
Plasmin Inhibitor

Modifications of α2-Plasmin Inhibitor During Treatments by a Defibrinating Agent

1979 ◽  
Author(s):  
M. Samama ◽  
J. Conard ◽  
B. Cazenave ◽  
A. Derlon ◽  
A. Gaudric ◽  
...  
Keyword(s):  
Retinal Vein Occlusion ◽  
Antithrombin Iii ◽  
Fibrinogen Level ◽  
Plasma Viscosity ◽  
Inhibitor Complex ◽  
Vein Occlusion ◽  
Crossed Immunoelectrophoresis ◽  
Α2 Macroglobulin ◽  
Plasmin Inhibitor ◽  
Soluble Complexes

A defibrination agent (Defibrase®) has been administered to 8 patients with retinal vein occlusion. Defibrase has been infused sub-cutaneously at a dose of 0.5 B. U./kg/day for 5 days followed by 1 to 2 B.U./kg twice a week, for 2 other weeks, so that fibrinogen level was maintained below 100 mg/100 ml. The tests performed were the following : fibrinogen, FDP, soluble complexes, plasminogen, α2 macroglobulin, antithrombin III, α2-plasmin inhibitor (amidolytic and Laurell methods), blood and plasma viscosity. They have been done before treatment and repeated daily for 5 days and then on the 8th, 11th, 14th and 21st day.A decrease in fibrinogen, viscosity, plasminogen and an increase in FDP and soluble complexes have been observed, as already described. The results of the α2- plasmin inhibitor shows a decrease of about 50 % that is slightly more pronounced by the Laurell method than the amidolytic method. The plasmin-α2 plasmin inhibitor complex detected by crossed immunoelectrophoresis is present in various amounts.

Cerebrospinal Fluid Tissue Factor and Thrombin-Antithrombin III Complex as Indicators of Tissue Injury After Subarachnoid Hemorrhage

Stroke ◽  
1997 ◽  
Vol 28 (9) ◽  
pp. 1666-1670 ◽  
Author(s):  
Yutaka Hirashima ◽  
Shin Nakamura ◽  
Michiyasu Suzuki ◽  
Masanori Kurimoto ◽  
Shunro Endo ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Subarachnoid Hemorrhage ◽  
Tissue Factor ◽  
Antithrombin Iii ◽  
Tissue Injury ◽  
Thrombin Antithrombin Iii Complex

A New Fluorogenic Substrate Method for Assay of Bacterial Endotoxins Using Limulus Hemocyte Lysate

1979 ◽  
Author(s):  
T Harada ◽  
M Ohki ◽  
M Niwa ◽  
S Iwanaga
Keyword(s):  
Antithrombin Iii ◽  
Assay Method ◽  
Sensitive Assay ◽  
Fluorogenic Substrate ◽  
Toxin Concentration ◽  
Amidase Activity ◽  
E Coli ◽  
Bacterial Endotoxins ◽  
Plasmin Inhibitor ◽  
Limulus Test

Limulus hemocyte lysate contains a proclotting enzyme, which is transformed to the active clotting enzyme in the presence of gram-negative bacterial endotoxins. The clotting enzyme coagulates a clottable protein, named coagulogen, contained also in the lysate. This gelation reaction of the lysate, named Limulus test, has been widely employed as a simple and very sensitive assay method for endotoxins. We developed a new fluorogenic substrate, Boc-Leu-Gly-Arg-4-methylcoumarin amide, for Limulus clotting enzyme and established an enzymatic assay method for endotoxins, using the substrate. Because the endotoxin mediates the activation of proclotting enzyme in the lysate, the measurement of amidase activity could be applicable for quantitation of the endotoxins. In fact, the amidase activity determined fluorometrically increased by increasing concentration of E. coli 0111: B4 endotoxin added to the lysate, and a linear relationship between the toxin concentration and the activity was observed in the range of 5X10-6to 5xl0-2 µg endotoxin. The method was a fifty times more sensitive than that of the Limulus test and was very reproducible. However, the method was not directly applicable for the assay of endotoxins in circulating blood, as the amidase activity was strongly inhibited by antithrombin III and α2-plasmin inhibitor. Thus, some pretreatment with heat or chloroform on plasma samples before the assay was required.

Is there a possible role for haemostasis in the development of perigraft reaction complicating the modified Blalock Taussig shunt?

2000 ◽  
Vol 10 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Ulrike Salzer-Muhar ◽  
Ingrid Pabinger-Fasching ◽  
Sophie Zacherl-Wightman
Keyword(s):  
Antithrombin Iii ◽  
Unusual Complication ◽  
Laboratory Findings ◽  
Thrombin Antithrombin Iii Complex ◽  
Blalock Taussig Shunt

AbstractThe perigraft reaction is an unusual complication found in patients in whom a modified Blalock Taussig shunt has been created using a polytetrafluoroethylene graft. We found that, in two infants, consistent laboratory findings during such a perigraft reaction were hypofibrinogenemia, increased levels of thrombin-antithrombin III complex, prothrombinfragment 1 and 2 and products of degradation of fibrin. Normalization of the levels of fibrinogen produced resolution of the perigraft reaction.

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