Biomechanical Analysis of Intrinsic Tendon Healing in Vitro and the Effects of Vitamins A and E

1991 ◽  
Vol 87 (5) ◽  
pp. 931-932
Author(s):  
G??ran Lundborg
Keyword(s):  
Tendon Healing ◽  
Biomechanical Analysis

Biomechanical Analysis of Intrinsic Tendon Healing in Vitro and the Effects of Vitamins A and E

1991 ◽  
Vol 87 (5) ◽  
pp. 925-930 ◽  
Author(s):  
Daniel Greenwald ◽  
Daniel Mass ◽  
Lawrence Gottlieb ◽  
Robert Tuel
Keyword(s):  
Tendon Healing ◽  
Biomechanical Analysis

Development of an in vitro Model for the Study of the Response of Equine Tendon Fibroblasts to Injury and Medication

1997 ◽  
Vol 10 (01) ◽  
pp. 6-11 ◽  
Author(s):  
R. F. Rosenbusch ◽  
L. C. Booth ◽  
L. A. Dahlgren
Keyword(s):  
Electron Microscopy ◽  
Extracellular Matrix ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Tendon Healing ◽  
Matrix Proteins ◽  
Isolated Cells ◽  
Superficial Digital Flexor Tendon ◽  
Vitro Model

SummaryEquine tendon fibroblasts were isolated from explants of superficial digital flexor tendon, subcultured and maintained in monolayers. The cells were characterized by light microscopy, electron microscopy and radiolabel studies for proteoglycan production. Two predominant cell morphologies were identified. The cells dedifferentiated toward a more spindle shape with repeated subcultures. Equine tendon fibroblasts were successfully cryopreserved and subsequently subcultured. The ability to produce proteoglycan was preserved.The isolated cells were identified as fibroblasts, based on their characteristic shape by light microscopy and ultrastructure and the active production of extracellular matrix proteins. Abundant rough endoplasmic reticulum and the production of extracellular matrix products demonstrated active protein production and export. Proteoglycans were measurable via liquid scintillation counting in both the cell-associated fraction and free in the supernatant. This model is currently being utilized to study the effects of polysulfated glycosaminoglycan on tendon healing. Future uses include studying the effects of other pharmaceuticals, such as hyaluronic acid, on tendon healing.A model was developed for in vitro investigations into tendon healing. Fibroblasts were isolated from equine superficial digital flexor tendons and maintained in monolayer culture. The tenocytes were characterized via light and electron microscopy. Proteoglycan production was measured, using radio-label techniques. The fibroblasts were cryopreserved and subsequently subcultured. The cells maintained their capacity for proteoglycan production, following repeated subculturing and cryopreservation.

An in vitro model of fibroblast activity and adhesion formation during flexor tendon healing

1994 ◽  
Vol 19 (5) ◽  
pp. 769-776 ◽  
Author(s):  
David L. Packer ◽  
George W. Dombi ◽  
Ping Yang Yu ◽  
Paul Zidel ◽  
Walter G. Sullivan
Keyword(s):  
In Vitro Model ◽  
Flexor Tendon ◽  
Adhesion Formation ◽  
Tendon Healing ◽  
Vitro Model

Flexor tendon healing in vitro: Effects of TGF-β on tendon cell collagen production

2002 ◽  
Vol 27 (4) ◽  
pp. 615-620 ◽  
Author(s):  
Matthew B. Klein ◽  
Naveen Yalamanchi ◽  
Hung Pham ◽  
Michael T. Longaker ◽  
James Chan
Keyword(s):  
Flexor Tendon ◽  
Tendon Healing ◽  
Collagen Production ◽  
Tendon Cell ◽  
In Vitro Effects

Comprehensive Biomechanical Analysis of Three Lumbopelvic Reconstruction Techniques following Total Sacrectomy: An In Vitro Human Cadaveric Model

2016 ◽  
Vol 16 (10) ◽  
pp. S161
Author(s):  
Bryan W. Cunningham ◽  
Mohamad Bydon ◽  
Ashley A. Murgatroyd ◽  
Kenneth Mullinix ◽  
Ziya L. Gokaslan ◽  
...  
Keyword(s):  
Biomechanical Analysis ◽  
Total Sacrectomy ◽  
Cadaveric Model

Effect of Hyaluronic Acid on Rabbit Profundus Flexor Tendon Healing in Vitro

1993 ◽  
Vol 55 (4) ◽  
pp. 411-415 ◽  
Author(s):  
Nader I. Salti ◽  
Robert J. Tuel ◽  
Daniel P. Mass
Keyword(s):  
Hyaluronic Acid ◽  
Flexor Tendon ◽  
Tendon Healing

scholarly journals Effects of Redox Modulation on Cell Proliferation, Viability, and Migration in Cultured Rat and Human Tendon Progenitor Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Yuk Wa Lee ◽  
Sai Chuen Fu ◽  
Man Yi Yeung ◽  
Chun Man Lawrence Lau ◽  
Kai Ming Chan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Vitamin C ◽  
Tendon Healing ◽  
In Vitro Study ◽  
Tendon Injury ◽  
Antioxidant Vitamin ◽  
Redox Modulation ◽  
And Migration

Tendon healing is slow and usually results in inferior fibrotic tissue formation. Recently, application of tendon derived stem cells (TDSCs) improved tendon healing in animal studies. In a chicken model, local injection of antioxidants reduced tendon adhesion after tendon injury. An in vitro study demonstrated that supplementation of H2O2reduced tenogenic marker expression in TDSCs. These findings suggested that the possibility of TDSCs is involved in tendon healing and the cellular activities of TDSCs might be affected by oxidative stress of the local environment. After tendon injury, oxidative stress is increased. Redox modulation might affect healing outcomes via affecting cellular activities in TDSCs. To study the effect of oxidative stress on TDSCs, the cellular activities of rat/human TDSCs were measured under different dosages of vitamin C or H2O2in this study. Lower dose of vitamin C increased cell proliferation, viability and migration; H2O2affected colony formation and suppressed cell migration, cell viability, apoptosis, and proliferation. Consistent with previous studies, oxidative stresses (H2O2) affect both recruitment and survival of TDSCs, while the antioxidant vitamin C may exert beneficial effects at low doses. In conclusion, redox modulation affected cellular activities of TDSCs and might be a potential strategy for tendon healing treatment.

Sign in / Sign up

Export Citation Format

Share Document