Reproducibility of Standard Preparation in Digoxin Radioimmunoassay in Plasma and Serum

1983 ◽  
Vol 5 (3) ◽  
pp. 355-358 ◽  
Author(s):  
L. Molin ◽  
B. Bergdahl ◽  
G. Dahlström ◽  
E. Leander
Keyword(s):  
Standard Preparation ◽  
Digoxin Radioimmunoassay

A in Vivo Assay for Standardizing Heparin Preparations

1979 ◽  
Vol 41 (03) ◽  
pp. 576-582
Author(s):  
A R Pomeroy
Keyword(s):  
In Vitro Assays ◽  
British Pharmacopoeia ◽  
In Vitro Method ◽  
Standard Preparation ◽  
Reliable Estimation ◽  
Assay Procedure ◽  
Accuracy And Precision ◽  
Heparin Preparation

SummaryThe limitations of currently used in vitro assays of heparin have demonstrated the need for an in vivo method suitable for routine use.The in vivo method which is described in this paper uses, for each heparin preparation, four groups of five mice which are injected intravenously with heparin according to a “2 and 2 dose assay” procedure. The method is relatively rapid, requiring 3 to 4 hours to test five heparin preparations against a standard preparation of heparin. Levels of accuracy and precision acceptable for the requirements of the British Pharmacopoeia are obtained by combining the results of 3 to 4 assays of a heparin preparation.The similarity of results obtained the in vivo method and the in vitro method of the British Pharmacopoeia for heparin preparations of lung and mucosal origin validates this in vivo method and, conversely, demonstrates that the in vitro method of the British Pharmacopoeia gives a reliable estimation of the in vivo activity of heparin.

scholarly journals Effect of Splinting in Accuracy of Two Implant Impression Techniques

2014 ◽  
Vol 40 (6) ◽  
pp. 633-639 ◽  
Author(s):  
Erica Dorigatti de Avila ◽  
Fernanda de Matos Moraes ◽  
Sabrina Maria Castanharo ◽  
Marcelo Antonialli Del'Acqua ◽  
Francisco de Assis Mollo
Keyword(s):  
Confidence Interval ◽  
Analysis Of Variance ◽  
Impression Material ◽  
Titanium Screw ◽  
Standard Preparation ◽  
Implant Abutment ◽  
Interface Gaps ◽  
Group 2 ◽  
Group 1

Because there is no consensus in the literature about the need for a splint between copings, the aim of this study was to evaluate, in vitro, the accuracy of 2 impression techniques for implant-supported prostheses. A master cast was fabricated with four parallel implant abutment analogs and a passive framework. Two groups with 5 casts each were formed: Group 1 (squared impression copings with no splint: S) and Group 2 (splinted squared impression copings, using metal drill burs and Pattern resin: SS). The impression material used was polyvinyl siloxane with open trays for standard preparation of the casts. For each cast, the framework was positioned, and a titanium screw was tightened with 10 N·cm torque in analog A, after which measurements of the abutment-framework interface gaps were performed at analogs C and D. This process was repeated for analog D. These measurements were analyzed using software. A one-way analysis of variance (ANOVA) with a confidence interval of 95% was used to analyze the data. Significant differences were detected between S and SS in relation to the master cast (P ≤ 0.05). The median values of the abutment-framework interface gaps were as follows: master cast: 39.64 μm; squared impression copings with no splint: 205.86 μm; splinted squared impression copings: 99.19 μm. Under the limitations of this study, the technique presented for Group 2 produces better results compared with the technique used for Group 1.

A BIOLOGICAL ASSAY FOR CALCITONIN

1965 ◽  
Vol 33 (3) ◽  
pp. 469-475 ◽  
Author(s):  
M. A. KUMAR ◽  
E. SLACK ◽  
A. EDWARDS ◽  
H. A. SOLIMAN ◽  
A. BAGHDIANTZ ◽  
...  
Keyword(s):  
Satisfactory Agreement ◽  
Intravenous Administration ◽  
Dose Range ◽  
Plasma Calcium ◽  
Dose Effect ◽  
Biological Assay ◽  
Potency Ratio ◽  
Standard Preparation ◽  
Assay Design ◽  
Routine Assay

SUMMARY (1) Calcitonin preparations from acetone-dried thyroid were administered to rats by various routes. (2) Intravenous administration, especially by infusion, produced a much greater fall in plasma calcium than s.c. or i.p. injection. (3) The log dose-effect curves after i.v. injection or infusion showed no evidence of non-linearity over a 100-fold dose range and had highly significant slopes. (4) The potency ratio of two preparations was estimated by means of a (2+2) assay design using both i.v. infusion and single i.v. injection. There was satisfactory agreement. (5) The i.v. injection method is recommended for the routine assay of calcitonin. A simple assay schedule is given in the Appendix. (6) A unit of calcitonin activity is defined in terms of a standard preparation.

On standards provided with digoxin radioimmunoassay kits.

1983 ◽  
Vol 29 (4) ◽  
pp. 734-735 ◽  
Author(s):  
L Molin ◽  
B Bergdahl
Keyword(s):  
Digoxin Radioimmunoassay

Reduced variation of tracer binding in digoxin radioimmunoassay by use of (125I)-labeled tyrosine-methyl-ester derivative: relation of thyroxine concentration to binding.

1976 ◽  
Vol 22 (10) ◽  
pp. 1732-1734 ◽  
Author(s):  
B H Kroening ◽  
M Weintraub
Keyword(s):  
Methyl Ester ◽  
Binding Proteins ◽  
Serum Samples ◽  
Methyl Ester Derivative ◽  
Ester Derivative ◽  
Free Serum ◽  
Digoxin Radioimmunoassay

Abstract Between-sample variation in tracer binding in the 125I-labeled digoxin radioimmunoassay was investigated with two tracers, 3-O-succinyl-digoxigenin-[125I]-labeled tyrosine and [125I]-labeled tyrosine-methyl-ester-digoxin. Digoxin-free serum samples having various concentrations of thyroxine were assayed with both tracers. The percentage of tracer bound when the samples were assayed with the first-mentioned tracer was increased significantly for the low thyroxine groups when compared to the normal (P less than 0.001) or the high thyroxine groups (P less than 0.05). Little difference existed when the latter tracer was used. There was variation in tracer binding when serum from dogs dosed with thyrotropin was assayed with the first tracer, but there was little variation with the second. Tracer binding may be influenced by thyroxine-binding proteins. Variation in tracer binding appears to be reduced when [125I]-labeled tyrosine-methyl-ester-digoxin is used.

FURTHER OBSERVATIONS ON THE OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1965 ◽  
Vol 32 (1) ◽  
pp. 1-7 ◽  
Author(s):  
E. T. BELL ◽  
J. A. LORAINE ◽  
S. MUKERJI ◽  
PACHARA VISUTAKUL
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Dose Response ◽  
Intravenous Route ◽  
Response Curves ◽  
Standard Preparation ◽  
Routes Of Administration ◽  
Seasonal Factors ◽  
Dose Response Curves ◽  
The Absolute

SUMMARY A modification of the ovarian ascorbic acid depletion (OAAD) method for luteinizing hormone (LH) is described in which the standard and test materials are administered by the intraperitoneal rather than by the intravenous route. The potency of NIH-LH when administered i.p. and i.v. was the same and the slopes of the log dose-response curves for the two routes of administration were very similar. Studies on the effect of seasonal factors on the OAAD method showed that both the slopes of the log dose-response curves and the absolute levels of ascorbic acid varied from one assay to another. The necessity for using a standard preparation in all assays of LH activity by the OAAD method is emphasized.

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