Abstract 10.55 PLATELET-RICH PLASMA VS. FETAL BOVINE SERUM IN ENGINEERING OF AXIALLY-VASCULARIZED OSTEOGENIC GRAFTS FROM HUMAN ADIPOSE-DERIVED CELLS TO TREAT AVASCULAR NECROSIS OF BONE

Abstrak - Mesenchymal Stem Cell (MSC) berpotensi untuk digunakan dalam pengobatan karena bersifat multipoten, dapat bermigrasi, dapat berproliferasi. MSC dikultur menggunakan media basal dengan penambahan suplemen, seperti Fetal Bovine Serum (FBS) dan Platelet-Rich Plasma(PRP) untuk mendukung pertumbuhannya. FBS dan PRP memiliki bahan dasar yang berbeda yaitu FBS berasal dari serum fetus sapi sedangkan PRP berasal dari plasma manusia, sehingga perlu dilakukan analisis karakter MSC yang dikultur pada media dengan penambahan FBS atau PRP. Tujuan penelitian adalah untuk menganalisis profil MSC yang dikultur pada media dengan penambahan FBS 10% atau PRP 10%. Metode yang dilakukan adalah isolasi MSC, pewarnaan MSC, penentuan populasi sel, dan analisis statistik. Penanda yang digunakan berupa CD73, CD90, CD105 dan Lineage negative (CD45/CD34/CD11b/CD19/HLA-DR). Sampel MSC didapatkan dari 8 orang pasien Klinik Hayandra. Hasil dari penelitian menunjukkan bahwa tidak ada perbedaan yang signifikan dari seluruh marker CD, yaitu Lin-(-)/CD90+ (p = 0,16), Lin-(+)/CD90+ (p = 0,43), dan CD105+/CD73+ (p = 0.5). Walaupun tidak memiliki perbedaan nyata, namun PRP cenderung memberikan hasil yang lebih baik daripada FBS, sehingga PRP dapat dijadikan sebagai suplemen alternatif dalam mengkultur MSC. Abstract - Mesenchymal Stem Cell (MSC) were used in many therapy in curing degenerative diseases. Because of its multipotent character, MSC could migrated, proliferated, and differentiated. In culture, MSC needed some suplements such as Fetal Bovine Serum (FBS) and Platelet-Rich Plasma (PRP) to support the growth. FBS and PRP had different ingredients based on their resources. FBS is derived from bovine fetus serum whereas PRP comes from human plasma, so became necessary to analyze MSC characters cultured in media with FBS or PRP suplements. The purpose of research was to analyze the MSC profile cultured with addition of 10% FBS and 10% PRP. The methodology were MSC isolation, MSC staining, cell population determination, and statistic analysis. The markers used are CD73, CD90, CD105 and Lineage negative (CD45 / CD34 / CD11b / CD19 / HLA-DR). Samples were obtained from 8 patients of Hayandra Clinic. The results of research showed that there were no significantly differences from all CD markers. Lin - (-) / CD90 + (p = 0.16), Lin - (+) / CD90 + (p = 0.43), and CD105 + / CD73 + (p = 0.5) gave different character in FBS or PRP suplementation. Although no significant difference, PRP tends to give better results than FBS in culturing MSC. This finding informed that PRP could be used as an alternative suplement in culturing MSC.Keywords - Mesenchymal Stem Cell (MSC), Fetal Bovine Serum (FBS), Platelet- Rich Plasma (PRP)

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Umbilical cord blood–derived platelet-rich plasma: a clinically acceptable substitute for fetal bovine serum?

Fertility and Sterility ◽

10.1016/j.fertnstert.2020.10.027 ◽

2020 ◽

Author(s):

Cristina Subiran ◽

Stine Gry Kristensen ◽

Claus Yding Andersen

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Bovine Serum ◽

Fetal Bovine Serum ◽

Platelet Rich Plasma ◽

Fetal Bovine

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The effect of activated pure platelete rich plasma (P-PRP) on the proliferation of adipose -derived mesenchymal stem cells (AD-MSCs) as a substitute for fetal bovine serum (FBS)

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2019.01.103 ◽

2019 ◽

Vol 20 (1) ◽

pp. 35-40

Author(s):

Aya El-Gamal

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bovine Serum ◽

Fetal Bovine Serum ◽

Platelet Rich Plasma ◽

Risk Of Infection ◽

Self Renewal ◽

Infection Transmission ◽

Fetal Bovine ◽

Subsequent Risk

The field of stem cells has attracted the attention of many researchers as a hope to treat many incurable diseases because of their ability of self-renewal and differentiation into a specific type of cells that compensates damaged cells. Many studies have been published to confirm their effectiveness but there are some obstacles that limit their clinical applications. One of these obstacles is xenogenic fetal bovine serum (FBS); the main proliferative source for stem cell culture with subsequent risk of infection transmission or immunogenic problems. So, in our study, we aimed to replace the xenogenic FBS with xeno-free blood derivative. We tested the effect of different concentrations of activated pure platelet rich plasma (P-PRP); one of the blood derivatives on proliferation of adipose derived mesenchymal stem cells (AD-MSCs) in comparison to FBS and found that 20% activated P-PRP followed by 10% activated P-PRP increased the proliferation rate of AD-MSCs more than 10% FBS.

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Comparative effect of platelet-rich plasma, platelet-poor plasma, and fetal bovine serum on the proliferative response of periodontal ligament cell subpopulations

Clinical Oral Investigations ◽

10.1007/s00784-018-2637-1 ◽

2018 ◽

Vol 23 (5) ◽

pp. 2455-2463 ◽

Cited By ~ 2

Author(s):

Constanza E. Martínez ◽

Roberto Gómez ◽

Alexis M. Kalergis ◽

Patricio C. Smith

Keyword(s):

Bovine Serum ◽

Periodontal Ligament ◽

Proliferative Response ◽

Fetal Bovine Serum ◽

Platelet Rich Plasma ◽

Comparative Effect ◽

Periodontal Ligament Cell ◽

Platelet Poor Plasma ◽

Fetal Bovine ◽

Cell Subpopulations

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Fetal Bovine Serum Substitute: Implication for Their Use in Translating Adipose-Derived Stem Cells from Bench to Bedside (Review)

Jurnal Plastik Rekonstruksi ◽

10.14228/jpr.v1i5.107 ◽

1970 ◽

Vol 1 (5) ◽

Author(s):

Fonny Josh ◽

Hiroshi Mizuno

Keyword(s):

Clinical Trials ◽

Bovine Serum ◽

Fetal Bovine Serum ◽

Platelet Rich Plasma ◽

Research Fields ◽

Cell Based Therapy ◽

Fetal Bovine ◽

Adipose Derived Stem Cell ◽

Serum Substitute

Recently, cell based therapy become a popular topic in research fields. This therapy offers great potential to treat many diseases. Adipose-derived stem cell (ASCs) is known to have the special characteristic due to its abundant availability and easily harvesting. Then it rapidly advanced into clinical trials for treatment of a broad range of medical conditions. Before translating ASCs from bench to bedside, this process is facing other problems through fetal bovine serum (FBS) elimination. Animal-derived serum is not a suitable option for ASCs expansion regarding the possibility of viral or bacterial infection and immune reaction. By replacing FBS with other non-animal serum which gives the same effect as FBS, may significantly enhances the safety and quality of ASCs. Pooled human AB serum (AB-HS), human serum (HS), pooled human plasma lysate (PL), umbilical cord serum (UCS)/placental serum (PS), thrombinactivated platelet rich plasma (tPRP) or serum free (SF)/xeno-free (XF) have been considered as FBS alternatives and some already applied in clinical trials with human cells. This article focuses on a prospective alternatives serum through the elimination of FBS.

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KONDROGENESIS ADIPOSE-DERIVED STEM CELLS MENGGUNAKAN PLATELET-RICH PLASMA PADA SCAFFOLD SUTRA

AL-Kauniyah Jurnal Biologi ◽

10.15408/kauniyah.v13i1.12053 ◽

2020 ◽

Vol 13 (1) ◽

pp. 31-38

Author(s):

Imam Rosadi ◽

Karina Karina ◽

Komang A. Wahyuningsih ◽

Iis Rosliana ◽

Tias Widyastuti ◽

...

Keyword(s):

Stem Cells ◽

Bombyx Mori ◽

Silk Fibroin ◽

Bovine Serum ◽

Fetal Bovine Serum ◽

Platelet Rich Plasma ◽

Adipose Derived Stem Cells ◽

Silk Fibroin Scaffold ◽

Salt Leaching ◽

Fetal Bovine

AbstrakStudi tentang kemampuan adipose-derived stem cells (ADSCs) sebagai sel punca yang dapat berdiferensiasi menjadi kondrosit menggunakan platelet-rich plasma (PRP) sebagai subtitusi fetal bovine serum (FBS) telah banyak dilaporkan. Penggunaan medium pertumbuhan dengan kombinasi ADSCs, PRP dan scaffold sutra masih belum banyak dipelajari dalam rekayasa jaringan kartilago. Studi ini bertujuan untuk mempelajari pengaruh medium yang mengandung 5%, 10% dan 20% PRP terhadap proses kondrogenesis ADSCs manusia yang dikultur pada scaffold sutra Bombyx mori Indonesia. Metode penelitian diawali dengan pembuatan scaffold sutra menggunakan metode salt-leaching, isolasi dan kultur ADSCs manusia dari jaringan lemak, uji pertumbuhan ADSCs pada scaffold sutra dengan variasi konsentrasi PRP pada medium serta analisis kadar glikosaminoglikan (GAG). Hasil penelitian menunjukkan bahwa ADSCs yang dikultur menggunakan PRP lebih tinggi laju pertumbuhannya dibandingkan dikultur menggunakan FBS selama 7 hari pengamatan. Kadar GAG yang disekresikan ADSCs kelompok PRP juga lebih tinggi dibandingkan kelompok FBS. Kadar GAG tertinggi pada hari ke-21 pengamatan adalah medium yang mengandung 20% PRP kemudian 10% dan 5%, sedangkan kadar GAG kelompok kontrol cenderung stabil pada kadar yang rendah. Berdasarkan hasil tersebut, medium yang mengandung PRP memiliki potensi dalam menginduksi kondrogenesis ADSCs yang dikultur pada scaffold sutra.Abstract The studies on adipose-derived stem cells (ADSCs) differentiation into chondrocytes using platelet-rich plasma (PRP) as a substitute for fetal bovine serum (FBS) have been reported. However, the combination of ADSCs, PRP and silk fibroin scaffold has not been widely studied for developing cartilage engineering. Therefore, this research aims to study the effect of medium containing 5%, 10% and 20% PRP towards chondrogenesis of human ADSCs cultured on silk fibroin scaffold from Indonesia Bombyx mori. At first, the silk fibroin scaffold was fabricated using a salt-leaching method, then ADSCs were isolated and cultured from adipose tissues. The assays of growth curve and biocompatibility of silk fibroin scaffold toward ADSCs supplemented by PRP as well as glycosaminoglycans (GAG) concentration were conducted later. The results showed that higher absorbance of proliferation rate was on ADSCs supplemented by various PRP concentrations compare to FBS control group for seven days of observation. Level of GAG, which secreted by ADSCs supplemented by a various concentration of PRP, was also higher than the FBS group. The highest level of GAG on day 21 was observed in 20% PRP group then 10% and 5% PRP, while a group of GAG level is stable at low levels. This study concludes that PRP has the potential to induce chondrogenesis ADSCs which cultured on silk fibroin scaffold.

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