Cynarha Daysy Cardoso da Silva
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Cristiane Moutinho Lagos de Melo
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Elba Verônica Matoso Maciel Carvalho
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Mércia Andréa Lino da Silva
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Rosiely Félix Bezerra
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...
Background:
Lectins have been studied in recent years due to their immunomodulatory
activities.
Objective:
We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we
analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes.
Methods:
Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations
of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine
incorporation, cytokines were investigated using ELISA assay and cell viability assay was
performed by investigation of damage through signals of apoptosis and necrosis.
Results:
OniL did not promote significant cell death, induced high mitogenic activity in relation to
control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines.
Conclusion:
These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent
in immunostimulatory assays.
COMPARISON OF TRITIATED THYMIDINE INCORPORATION ASSAY AND AN IN VITRO SUPPRESSION ASSAY OF CD4+CD25-T EFFECTOR CELL PROLIFERATION BY TH2-LIKE TREG AND NAÏVE TREG USING A FLOW CYTOMETRY BASED ASSAY
