scholarly journals The Omega Plate Technique

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Colin J. Anderson ◽  
Olivia M. Rice ◽  
Michael S. Schallmo ◽  
Joshua C. Patt ◽  
Jeffrey S. Kneisl
Keyword(s):  
Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 424
Author(s):  
Osama G. Mohamed ◽  
Sadaf Dorandish ◽  
Rebecca Lindow ◽  
Megan Steltz ◽  
Ifrah Shoukat ◽  
...  

The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth®, we isolated over 1400 antibiotic-producing microbes, including 62, showing activity against multidrug-resistant pathogens. The natural product extracts generated from six microbial isolates showed potent activity against vancomycin-intermediate resistant Staphylococcus aureus. We utilized a targeted approach that coupled mass spectrometry data with bioactivity, yielding a new macrolactone class of metabolite, desertomycin H. In this study, we successfully demonstrate a concept that significantly increased our ability to quickly and efficiently identify microbes capable of the silent antibiotic production.


1977 ◽  
Vol 11 (2) ◽  
pp. 225-228 ◽  
Author(s):  
P. F. Dougherty ◽  
D. W. Yotter ◽  
T. R. Matthews

2012 ◽  
Vol 51 (3) ◽  
pp. 299-307 ◽  
Author(s):  
Alexander Brunner ◽  
Jochen Müller ◽  
Pietro Regazzoni ◽  
Reto Babst

2011 ◽  
Vol 356-360 ◽  
pp. 2678-2681 ◽  
Author(s):  
Ji Zhao ◽  
Yu Qin Shao ◽  
Zi Long Zhao ◽  
Zheng Min Li ◽  
Xiao Tong Wu ◽  
...  

In this study, the composition and distribution of dominant soil filamentous microbes , such as fungi and actinomycetes, at dry lake wetland soil on typical steppe were measured through Spread-Plate-Technique; the divergent characteristics of two microbial floras at lake wetland and typical steppe were analyzed; and several indicators of soil filamentous microbes, for example, diversity, richness, evenness, and dominance, were calculated. For fungi, there is significant difference between lake center (LC), typical steppe (TS) & lake bed (LB), lake side (LS) for diversity and richness; and there is significant difference between LC and TS& LB and LS for dominance. For actinomycetes, there is significant difference between LC & LS, and LB& TS for diversity; and there is significant difference between LC&TS for richness; and there is significant difference between LC&LS for evenness. At LC, the most dominant floras is Mucor, accounting for 100%. At LB, Mucor accounts for 26.25% and Penicillium accounts for 45%. At LS, the dominant floras are Mucor, accounting for 48%, and Penicillium, accounting for 34.48%. At TS, Penicillium has become the dominant flora to account for 92.23%. For actinomycetes, the most dominant floras at LC is Griseofuscus, accounting for 55.71%. At LS, the dominant floras are Griseofuscus accounting for18.75%, and balding group, accounting for 20%. At TS, the dominant flora are Griseofuscus, accounting for16.08%, and Flav us, accounting for 14.69%.The results have demonstrated a better understanding toward the development and succession mechanisms of wetland to provide the basis for protection and rational utilizations of dry lake wetland.


1993 ◽  
Vol 56 (4) ◽  
pp. 971-972 ◽  
Author(s):  
Hitoshi Yokoyama ◽  
Takao Togo ◽  
Sadayuki Murata ◽  
Kenji Osaka ◽  
Makoto Miura ◽  
...  

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