CHORIORETINAL BIOPSY FOR THE DIAGNOSIS OF ENDOGENOUS ENDOPHTHALMITIS DUE TO ESCHERICHIA COLI

Nous rapportons le cas d’une femme de 60 ans, diabétique, hospitalisée pour hépatomégalie douloureuse fébrile. Le bilan d’entrée a montré une hyperleucocytose à PNN, une anémie normocytaire et un syndrome inflammatoire marqué. L’imagerie était évocatrice d’un abcès hépatique. Devant ce tableau clinique, une antibiothérapie probabiliste a été débutée. Trois jours plus tard, la patiente avait présenté un oeil rouge douloureux avec une baisse de l’acuité visuelle dont l’examen ophtalmologique avait retrouvé une infiltration cornéenne et hypopion. L’échographie en mode B avait montré un vitré très chargé en faveur d’une endophtalmie endogène. Une antibiothérapie à bonne diffusion oculaire était instaurée immédia-tement par voie générale et injection intravitréenne. La vitrectomie a été retardée du fait de mauvais état général de la patiente. Les hémocultures réalisées isolaient un Escherichia coli. L’évolution était marquée par la guérison de l’abcès hépatique mais l’acuité visuelle reste limitée à la perception lumineuse. Malgré la rareté de cette affection qui survient en général dans un contexte d’immunodépression, elle doit être évoquée devant tout signe oculaire associé à un foyer infectieux profond. Un diagnostic rapide et un traitement adéquat peuvent améliorer le pronostic vital et visuel.

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A case of bilateral Escherichia coli endogenous endophthalmitis

The American Journal of Medicine ◽

10.1016/0002-9343(76)90196-0 ◽

1976 ◽

Vol 61 (2) ◽

pp. A73

Keyword(s):

Escherichia Coli ◽

Endogenous Endophthalmitis

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Bilateral endogenous endophthalmitis complicated by scleral perforation: an unusual presentation

BMJ Case Reports ◽

10.1136/bcr-2021-244547 ◽

2021 ◽

Vol 14 (9) ◽

pp. e244547

Author(s):

Prerna Sinha ◽

Upasna Sinha ◽

Amit Raj ◽

Binod Kumar Pati

Keyword(s):

Escherichia Coli ◽

Female Patient ◽

Clinical Picture ◽

Vision Loss ◽

Orbital Cellulitis ◽

Endogenous Endophthalmitis ◽

Atypical Presentation ◽

Topical Antibiotics ◽

Culture And Sensitivity ◽

Autoimmune Aetiology

Endogenous endophthalmitis complicated by necrotising scleritis has rarely been reported in the literature. We, hereby, report a case of bilateral scleral perforation with endogenous endophthalmitis in an 87-year-old female patient with diabetes who presented as bilateral orbital cellulitis. Systemic workup ruled out autoimmune aetiology. The culture and sensitivity of exudates exuding from the scleral perforation showed Escherichia coli. The Patient was managed conservatively with parenteral and topical antibiotics along with steroid, but the vision could not be salvaged. The report emphasizes on atypical presentation of endogenous endophthalmitis. In old and immunosuppressed individuals presenting with a clinical picture of bilateral orbital cellulitis with profound vision loss, endogenous endophthalmitis should be ruled out.

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Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081693 ◽

1978 ◽

Vol 36 (2) ◽

pp. 166-167 ◽

Cited By ~ 1

Author(s):

G. Stöffler ◽

R.W. Bald ◽

J. Dieckhoff ◽

H. Eckhard ◽

R. Lührmann ◽

...

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Ribosomal Proteins ◽

Structural Organization ◽

Three Dimensional ◽

Ribosomal Subunit ◽

Spatial Arrangement ◽

Small Subunit ◽

Antibody Binding ◽

Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

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Sites of Adsorption of the Bacteriophages T3 and T5 on the Wall of Escherichia Coli B.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060490 ◽

1967 ◽

Vol 25 ◽

pp. 96-97

Author(s):

Manfred E. Bayer

Keyword(s):

Escherichia Coli ◽

Cell Wall ◽

Electron Microscope ◽

Uranyl Acetate ◽

E Coli ◽

Receptor Sites ◽

Rigid Cell Wall ◽

Host Cell Surface ◽

Bacterial Viruses ◽

Escherichia Coli B

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.

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Infection of Escherichia coli with bacteriophages

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100063767 ◽

1969 ◽

Vol 27 ◽

pp. 370-371

Author(s):

Manfred E. Bayer

Keyword(s):

Escherichia Coli ◽

Nucleic Acid ◽

Cell Surface ◽

Virus Type ◽

Infection Process ◽

Adsorption Site ◽

The Other ◽

Specific Receptors ◽

Bacterial Receptors

The first step in the infection of a bacterium by a virus consists of a collision between cell and bacteriophage. The presence of virus-specific receptors on the cell surface will trigger a number of events leading eventually to release of the phage nucleic acid. The execution of the various "steps" in the infection process varies from one virus-type to the other, depending on the anatomy of the virus. Small viruses like ØX 174 and MS2 adsorb directly with their capsid to the bacterial receptors, while other phages possess attachment organelles of varying complexity. In bacteriophages T3 (Fig. 1) and T7 the small conical processes of their heads point toward the adsorption site; a welldefined baseplate is attached to the head of P22; heads without baseplates are not infective.

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The Nature of the Intramembrane Particle

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600003020 ◽

1980 ◽

Vol 38 ◽

pp. 688-691

Author(s):

A.J. Verkleij

Keyword(s):

Escherichia Coli ◽

Tight Junction ◽

Gap Junction ◽

The Other ◽

Fracture Face ◽

Band 3 Protein ◽

Satisfactory Explanation ◽

Freeze Fracturing ◽

Intramembrane Particle ◽

Luminal Membrane

Freeze-fracturing splits membranes into two helves, thus allowing an examination of the membrane interior. The 5-10 rm particles visible on both monolayers are widely assumed to be proteinaceous in nature. Most membranes do not reveal impressions complementary to particles on the opposite fracture face, if the membranes are fractured under conditions without etching. Even if it is considered that shadowing, contamination or fracturing itself might obscure complementary pits', there is no satisfactory explanation why under similar physical circimstances matching halves of other membranes can be visualized. A prominent example of uncomplementarity is found in the erythrocyte manbrane. It is wall established that band 3 protein and possibly glycophorin represents these nonccmplanentary particles. On the other hand a number of membrane types show pits opposite the particles. Scme well known examples are the ";gap junction',"; tight junction, the luminal membrane of the bladder epithelial cells and the outer membrane of Escherichia coli.

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