Conditioned Medium From Mesenchymal Stem Cells Enhances Early Bone Regeneration After Maxillary Sinus Floor Elevation in Rabbits

2015 ◽  
pp. 1 ◽  
Author(s):  
Wataru Katagiri ◽  
Masashi Osugi ◽  
Kazuhiko Kinoshita ◽  
Hideharu Hibi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Maxillary Sinus ◽  
Conditioned Medium ◽  
Sinus Floor Elevation ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Maxillary Sinus Floor Elevation

scholarly journals Effects of Platelet-rich Fibrin on Osteogenic Differentiation of Schneiderian Membrane Derived Mesenchymal Stem Cells and Bone Formation in Maxillary Sinus

2021 ◽  
Author(s):  
Yanmin Zhou ◽  
Jia Wang ◽  
Yue Sun ◽  
Yiping Liu ◽  
Jize Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Maxillary Sinus ◽  
Sinus Floor Elevation ◽  
Schneiderian Membrane ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Maxillary Sinus Floor Elevation

Abstract BackgroundThe existence of mesenchymal stem cells (MSCs) in Schneiderian membrane has not been determined. The aim of this study is to investigate whether there are MSCs in Schneiderian membrane, and the effect of platelet-rich fibrin (PRF) on osteogenic differentiation of these cells and on new bone formation in maxillary sinus after maxillary sinus floor elevation.MethodsSchneiderian membrane derived mesenchymal stem cells (SM-MSCs) were isolate from rabbit maxillary sinus. Cells were identified by flow cytometry and multipotential differentiation. Real-time cell analysis assay, fluorescence staining, transwell assay, and wound healing assay were used to determine the effects of PRF stimulation on cell proliferation and migration. The osteogenic differentiation ability of cells stimulated by PRF or osteoinductive medium (OM) was evaluated by alkaline phosphatase (ALP) staining, alizarin red staining, PCR and Western blot. Equivalent volume Bio-oss and the mixture of Bio-oss and PRF were used as bone graft materials for maxillary sinus floor elevation. Micro-CT, bone double-staining, HE staining, Masson staining, and toluidine blue staining were used to evaluate the osteogenic effect in 8 and 12 weeks after surgery.ResultsThe cell surface markers were positive for expression of CD90, CD105, and negative for expression of CD34, CD45. SM-MSCs had the ability of osteogenic, adipogenic and chondrogenic differentiation. PRF can stimulate proliferation, migration and osteogenic differentiation of SM-MSCs, which is achieved by up-regulating ERK 1/2 signaling pathway. PRF can accelerate the formation of new bone in maxillary sinus and increase the amount of new bone formation. ConclusionsMSCs exist in Schneiderian membrane, and PRF stimulation can promote cell proliferation, migration and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation can accelerate bone healing and increase the quantity and quality of new bone.

scholarly journals The Human Amniotic Mesenchymal Stem Cells (hAMSCs) Improve the Implant Osseointegration and Bone Regeneration in Maxillary Sinus Floor Elevation in Rabbits

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Lu Yin ◽  
Zhi-xuan Zhou ◽  
Ming Shen ◽  
Ning Chen ◽  
Fei Jiang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Maxillary Sinus ◽  
Bone Volume ◽  
Control Group ◽  
Sinus Floor Elevation ◽  
Dental Implantation ◽  
Posterior Maxilla ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Maxillary Sinus Floor Elevation

Insufficient bone height in the posterior maxilla is a challenging problem in dental implantation. Bio-Oss, though routinely used in maxillary sinus floor elevation (MSFE), is not osteoinductive. Human amniotic mesenchymal cells (hAMSCs) isolated from placental tissues have potential for multidifferentiation and immunomodulatory properties and can be easily obtained without the need for invasive procedures and without ethical concerns. This is the first study to use hAMSCs to improve implant osseointegration and bone regeneration after MSFE. Human AMSCs were loaded into a fibrin gel and injected into rabbit MSFE models. The rabbits were assigned to four groups (n=3 per group), i.e., the control group, the hAMSC group, the Bio-Oss group, and the hAMSC/Bio-Oss group. The animals were sacrificed at postsurgery for four and twelve weeks and evaluated by histology and immunohistochemistry. Bone volume, bone volume/tissue volume, bone-to-implant contact ratio, and vessel-like structures in the hAMSC/Bio-Oss group were significantly better than those in other groups in the peri-implant and augmented areas. Immunofluorescence staining showed that alkaline phosphatase (ALP) activities of two hAMSC groups were higher than those of the other two groups. Sequential fluorescent labeling was performed in all of the 12-week groups. Observations showed that hAMSCs accelerated mineralized deposition rates on implant surfaces and in bone-augmented areas. These data demonstrated that hAMSCs could enhance implant osseointegration and bone regeneration after MSFE and might be used to optimize dental implantation in the future.

scholarly journals Maxillary Sinus Floor Elevation for Dental Implants: Revision Results of Research Oriented to Bone Regeneration

2018 ◽  
Vol 1 (2) ◽  
pp. 123-128
Author(s):  
Sergio Olate
Keyword(s):  
Surgical Technique ◽  
Bone Regeneration ◽  
Maxillary Sinus ◽  
Dental Implants ◽  
Sinus Floor Elevation ◽  
Research Teams ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Research Criteria ◽  
Maxillary Sinus Floor Elevation

Research oriented to bone regeneration and reconstruction has been growing and national and international research teams have complied with the development of sustained and sustainable research criteria. The aim of this work is to present the results of investigations realized by different centers to which the author is affiliated, making an analysis of maxillary sinus reconstruction for dental implants. We revised research during the last 6 years related to the surgical technique, anatomical analyses, and definition, as well as choice of bone graft. We determined the main conclusions of the analyses, innovative research to optimize efficiency in major sinus reconstruction was established, as was the technique modification for different cases.

Human Maxillary Sinus Floor Elevation as a Model for Bone Regeneration Enabling the Application of One-Step Surgical Procedures

2013 ◽  
Vol 19 (1) ◽  
pp. 69-82 ◽  
Author(s):  
Elisabet Farré-Guasch ◽  
Henk-Jan Prins ◽  
Janice R. Overman ◽  
Christiaan M. ten Bruggenkate ◽  
Engelbert A.J.M. Schulten ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Maxillary Sinus ◽  
Surgical Procedures ◽  
Sinus Floor Elevation ◽  
One Step ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Maxillary Sinus Floor Elevation
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