“Equivocal” High-Risk HPV DNA Tests Performed on ThinPrep Specimens After ASC-US Diagnoses Are Associated With an Increased Incidence of CIN3

2014 ◽  
Vol 18 (4) ◽  
pp. 291-297
Author(s):  
Kim HooKim ◽  
Richard W. Lieberman ◽  
Brian M. Smola ◽  
Stewart M. Knoepp
Keyword(s):  
High Risk ◽  
Hpv Dna ◽  
Hpv Dna Tests ◽  
High Risk Hpv

Comparison of Human Papillomavirus Detection and Typing by Hybrid Capture 2, Linear Array, DNA Chip, and Cycle Sequencing in Cervical Swab Samples

2009 ◽  
Vol 19 (2) ◽  
pp. 266-272 ◽  
Author(s):  
Jae Kwan Lee ◽  
Mi Kyung Kim ◽  
Seung Hun Song ◽  
Jin Hwa Hong ◽  
Kyung Jin Min ◽  
...  
Keyword(s):  
High Risk ◽  
Linear Array ◽  
Intraepithelial Neoplasia ◽  
Dna Chip ◽  
Substantial Agreement ◽  
Cervical Lesions ◽  
Cycle Sequencing ◽  
Hpv Dna ◽  
Hpv Dna Tests ◽  
High Risk Hpv

Although the Hybrid Capture II (HC II) assay can detect 13 high-risk human papillomavirus (HPVs), it does not yield any genotype-specific information. We evaluated the performance of 4 HPV DNA tests, namely, HC II, Linear Array (LA), DNA chip, and cycle sequencing for their capacity to detect the presence of high-risk HPV DNA and HPV-associated cervical lesions. Seventy-six women who were referred to the colposcopy clinic for abnormal cytology were enrolled. The women were examined using liquid-based cytology, colposcopy-directed biopsy, and HPV DNA tests. After DNA extraction from a single sample, HPV DNA tests were performed by all 4 methods on the same specimen. The LA test has higher HPV-positive rates than HC II for cervical intraepithelial neoplasia I (83.3% vs 61.1%;P< 0.01) and for cervical intraepithelial neoplasia II and more severe lesions (100.0% vs 80.0%;P< 0.01). The concordance between the DNA chip and LA tests was 89.5%, confirming substantial agreement (κcoefficient = 0.73), and the concordance between HC II and the DNA chip was 80.3%, also showing substantial agreement (κcoefficient = 0.738). The concordance for 15 high-risk HPV genotypes between LA and sequencing was 82.5% with aκvalue of 0.536. Furthermore, the LA test was more sensitive in the detection of high-grade cervical lesions than HC II (100% vs 92.3%,P< 0.01). The LA test showed superior sensitivity in the detection of clinically relevant HPV infections and has proven to be an accurate tool for identifying individual HPV types, especially in cases of multiple HPV infections.

Comparison of Abbott RealTime High Risk HPV and Hybrid Capture 2 for the detection of high-risk HPV DNA in a referral population setting

2012 ◽  
Vol 53 (2) ◽  
pp. 121-124 ◽  
Author(s):  
Simona Venturoli ◽  
Elisa Leo ◽  
Martina Nocera ◽  
Daniela Barbieri ◽  
Monica Cricca ◽  
...  
Keyword(s):  
High Risk ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Hybrid Capture 2 ◽  
High Risk Hpv

scholarly journals p16 expression in cutaneous squamous cell carcinoma of the head and neck is not associated with integration of high risk HPV DNA or prognosis

Pathology ◽  
2017 ◽  
Vol 49 (5) ◽  
pp. 494-498 ◽  
Author(s):  
Laveniya Satgunaseelan ◽  
Noel Chia ◽  
Hyerim Suh ◽  
Sohaib Virk ◽  
Bruce Ashford ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
High Risk ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Hpv Dna ◽  
High Risk Hpv ◽  
P16 Expression

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

BioFactors ◽  
2018 ◽  
Vol 45 (2) ◽  
pp. 101-117 ◽  
Author(s):  
Pegah Mahmoodi ◽  
Mona Fani ◽  
Majid Rezayi ◽  
Amir Avan ◽  
Zahra Pasdar ◽  
...  
Keyword(s):  
Systematic Review ◽  
Cervical Cancer ◽  
High Risk ◽  
Early Detection ◽  
Hpv Dna ◽  
High Risk Hpv

scholarly journals Infección por HPV de alto riesgo y lesiones intraepiteliales anales en hombres HIV positivos que tienen sexo con hombres

2018 ◽  
Author(s):  
Valeria Fink ◽  
Laura Svidler López ◽  
Fernanda González ◽  
Mariana Tejo ◽  
Gisela Presencia ◽  
...  
Keyword(s):  
High Risk ◽  
Hpv 16 ◽  
Hpv Dna ◽  
High Risk Hpv

Introducción: El cáncer anal, asociado a la infección con virus de papiloma humano de alto riesgo (HPV-AR), es muy frecuente en hombres que tienen sexo con hombres (HSH) HIV+. Objetivo: Evaluar frecuencia de infección por HPV-AR, genotipos y lesiones asociadas, y factores asociados. Materiales y métodos: Estudio en HSH HIV+ (septiembre 2012-marzo 2014, Hospital Fernández). Se recogió información demográfica, de HIV, HPV y prácticas sexuales. Se realizó citología anal, detección de HPV-AR (HC2 High- Risk HPV DNA, Digene®) y genotipificación en las muestras HPV-AR+ (Inno Lipa®, Fujirebio). Los pacientes firmaron consentimiento informado. Se indicó tratamiento según resultados. Resultados: Completaron el estudio 57 pacientes. Mediana de edad: 40 años (rango intercuartil [RIC]: 29-45); de CD4: 444 cels/mm3 (RIC: 345-568); 77% recibían tratamiento antirretroviral, 68% con carga viral no detectable. Citologías: negativas (24%); lesión intraepitelial de bajo grado (54%); lesión intraepitelial de alto grado (20%); ASCUS (2%). El 80% fue HPV-AR+. Los pacientes con diagnóstico de HPV-AR (p=0,006) y de lesión intraepitelial tuvieron CD4 <500 cels/ mm3 con más frecuencia (p=0,030). Los pacientes con HPV-AR tuvieron mayor frecuencia de carga viral detectable (p=0,020, prueba de Fisher). El porcentaje de pacientes con uso consistente de preservativo fue mayor entre los pacientes sin lesión citológica (p=0,026). Genotipos de alto riesgo más frecuentes: HPV-16 (51%), HPV-31 (44%) y HPV-51 (40%); de bajo riesgo: HPV-6 (47%) y HPV-44 (35%). Conclusiones: Se encontró elevada frecuencia de lesión citológica (76%) y de HPV-AR (80%). Es necesario establecer estrategias de prevención en esta población incluyendo tamizaje, vacunación y promoción de sexo seguro.

Detection of high-risk HPV DNA in semen and its association with the quality of semen

2004 ◽  
Vol 15 (11) ◽  
pp. 740-743 ◽  
Author(s):  
M A M Rintala ◽  
S E Greénman ◽  
P P Pöllänen ◽  
J J O Suominen ◽  
S M Syrjänen
Keyword(s):  
High Risk ◽  
Hpv Dna ◽  
High Risk Hpv

scholarly journals Detection and estimation of human papillomavirus viral load in patients with cervical lesions

2014 ◽  
Vol 39 (2) ◽  
pp. 86-90 ◽  
Author(s):  
T Rahman ◽  
S Tabassum ◽  
M Jahan ◽  
A Nessa ◽  
Dr Ashrafunnessa
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
High Risk ◽  
Invasive Cervical Cancer ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Promising Tool ◽  
High Risk Hpv ◽  
Hpv Viral Load

Human papillomavirus (HPV) high risk genotype infection and HPV viral load influences the development of invasive cervical cancer and cervical intra-epithelial neoplasia (CIN). HPV DNA testing for screening of cervical cancers may play a potential role in its early detection and management. The present study detected HPV DNA and estimated HPV viral load in different types of cervical lesions among Bangladeshi women. Using the Hybrid Capture 2 (HC2) assay, HPV DNA was tested among 68 women between 25-70 years of age. A total of 13 (19.1%) cases were positive for HPV DNA. The highest viral load (501 x 10³ copies/ml) was detected in a patient with invasive carcinoma, while the lowest viral load (105 x 10³ copies/ml) was detected from a case of chronic cervicitis. The mean viral load in CIN I was 119.25 x 10³±12.5 x 10³ copies/ml (range: 110 x 10³ - 137 x 10³) and 208.50 x 10³ ± 0.59 x 10³ copies/ml (range: 139 x 10³-305 x 10³) in CIN II / III. Interestingly, HPV DNA was detected from a patient with normal cytological findings. Our study observed a moderate presence of high-risk HPV genotypes among women with cervical lesions. The HPV viral load varied with the age of the patients and stage of cervical lesions. The HC2 assay is a promising tool for diagnosing high-risk HPV infection especially before cytology tests show any abnormality. DOI: http://dx.doi.org/10.3329/bmrcb.v39i2.19648 Bangladesh Med Res Counc Bull 2013; 39: 86-90

S100-A8 EXPRESSION IS SIGNIFICANTLY ASSOCIATED WITH HIGH-RISK HPV DNA POSITIVITY AND A WORSE SURVIVAL IN ORAL CANCER

2020 ◽  
Vol 130 (3) ◽  
pp. e240-e241
Author(s):  
MARISOL MIRANDA GALVIS ◽  
CAROLINA CARNEIRO SOARES ◽  
FABIO A. MARCHI ◽  
ALAN ROGER SANTOS-SILVA ◽  
LUIZ PAULO KOWALSKI ◽  
...  
Keyword(s):  
High Risk ◽  
Oral Cancer ◽  
Hpv Dna ◽  
High Risk Hpv
Sign in / Sign up

Export Citation Format

Share Document