S100-A8 EXPRESSION IS SIGNIFICANTLY ASSOCIATED WITH HIGH-RISK HPV DNA POSITIVITY AND A WORSE SURVIVAL IN ORAL CANCER

The relation between HPV and head and neck cancer has recently and extensively been investigated. The purpose of this study was to indentify HPV genotypes, as well as E6/E7 mRNA expression of high-risk HPVs (16, 18, 31, 33 and 45) in oral squamous cell carcinomas (OSCCs) from 45 Greek patients. The overall prevalence of HPV DNA positive OSCCs was 11.1% (5/45), while high-risk HPV DNA was found in 6.7% (3/45) of OSCCs. E6/E7 mRNA expression was detected in 8.9% (4/45) of the oral cavity samples. Our data indicated that HPV 16 was the commonest genotype identified in HPV-positive OSCCs by both DNA and RNA tests. This study confirms the prevalence of HPV infections among patients with OSCCs. Future analysis and followup of more OSCCs will enable us to correlate HPV detection and clinical outcome.

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Comparison of Human Papillomavirus (HPV) detection in urine and cervical samples using high-risk HPV DNA testing in Northern Thailand

10.26226/morressier.578f37fad462b8028d88f8f5 ◽

2016 ◽

Author(s):

Surapan Khunamornpong

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Dna Testing ◽

Northern Thailand ◽

Hpv Dna ◽

Hpv Dna Testing ◽

High Risk Hpv

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Comparison of Abbott RealTime High Risk HPV and Hybrid Capture 2 for the detection of high-risk HPV DNA in a referral population setting

Journal of Clinical Virology ◽

10.1016/j.jcv.2011.10.016 ◽

2012 ◽

Vol 53 (2) ◽

pp. 121-124 ◽

Cited By ~ 10

Author(s):

Simona Venturoli ◽

Elisa Leo ◽

Martina Nocera ◽

Daniela Barbieri ◽

Monica Cricca ◽

...

Keyword(s):

High Risk ◽

Hybrid Capture ◽

Hpv Dna ◽

Hybrid Capture 2 ◽

High Risk Hpv

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p16 expression in cutaneous squamous cell carcinoma of the head and neck is not associated with integration of high risk HPV DNA or prognosis

Pathology ◽

10.1016/j.pathol.2017.04.002 ◽

2017 ◽

Vol 49 (5) ◽

pp. 494-498 ◽

Cited By ~ 16

Author(s):

Laveniya Satgunaseelan ◽

Noel Chia ◽

Hyerim Suh ◽

Sohaib Virk ◽

Bruce Ashford ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

High Risk ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Cutaneous Squamous Cell Carcinoma ◽

Hpv Dna ◽

High Risk Hpv ◽

P16 Expression

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Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

BioFactors ◽

10.1002/biof.1465 ◽

2018 ◽

Vol 45 (2) ◽

pp. 101-117 ◽

Cited By ~ 10

Author(s):

Pegah Mahmoodi ◽

Mona Fani ◽

Majid Rezayi ◽

Amir Avan ◽

Zahra Pasdar ◽

...

Keyword(s):

Systematic Review ◽

Cervical Cancer ◽

High Risk ◽

Early Detection ◽

Hpv Dna ◽

High Risk Hpv

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Comparison of Human Papillomavirus Detection and Typing by Hybrid Capture 2, Linear Array, DNA Chip, and Cycle Sequencing in Cervical Swab Samples

International Journal of Gynecological Cancer ◽

10.1111/igc.0b013e31819bcd0a ◽

2009 ◽

Vol 19 (2) ◽

pp. 266-272 ◽

Cited By ~ 16

Author(s):

Jae Kwan Lee ◽

Mi Kyung Kim ◽

Seung Hun Song ◽

Jin Hwa Hong ◽

Kyung Jin Min ◽

...

Keyword(s):

High Risk ◽

Linear Array ◽

Intraepithelial Neoplasia ◽

Dna Chip ◽

Substantial Agreement ◽

Cervical Lesions ◽

Cycle Sequencing ◽

Hpv Dna ◽

Hpv Dna Tests ◽

High Risk Hpv

Although the Hybrid Capture II (HC II) assay can detect 13 high-risk human papillomavirus (HPVs), it does not yield any genotype-specific information. We evaluated the performance of 4 HPV DNA tests, namely, HC II, Linear Array (LA), DNA chip, and cycle sequencing for their capacity to detect the presence of high-risk HPV DNA and HPV-associated cervical lesions. Seventy-six women who were referred to the colposcopy clinic for abnormal cytology were enrolled. The women were examined using liquid-based cytology, colposcopy-directed biopsy, and HPV DNA tests. After DNA extraction from a single sample, HPV DNA tests were performed by all 4 methods on the same specimen. The LA test has higher HPV-positive rates than HC II for cervical intraepithelial neoplasia I (83.3% vs 61.1%;P< 0.01) and for cervical intraepithelial neoplasia II and more severe lesions (100.0% vs 80.0%;P< 0.01). The concordance between the DNA chip and LA tests was 89.5%, confirming substantial agreement (κcoefficient = 0.73), and the concordance between HC II and the DNA chip was 80.3%, also showing substantial agreement (κcoefficient = 0.738). The concordance for 15 high-risk HPV genotypes between LA and sequencing was 82.5% with aκvalue of 0.536. Furthermore, the LA test was more sensitive in the detection of high-grade cervical lesions than HC II (100% vs 92.3%,P< 0.01). The LA test showed superior sensitivity in the detection of clinically relevant HPV infections and has proven to be an accurate tool for identifying individual HPV types, especially in cases of multiple HPV infections.

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Infección por HPV de alto riesgo y lesiones intraepiteliales anales en hombres HIV positivos que tienen sexo con hombres

Actualizaciones en Sida e Infectología ◽

10.52226/revista.v26i97.29 ◽

2018 ◽

Author(s):

Valeria Fink ◽

Laura Svidler López ◽

Fernanda González ◽

Mariana Tejo ◽

Gisela Presencia ◽

...

Keyword(s):

High Risk ◽

Hpv 16 ◽

Hpv Dna ◽

High Risk Hpv

Introducción: El cáncer anal, asociado a la infección con virus de papiloma humano de alto riesgo (HPV-AR), es muy frecuente en hombres que tienen sexo con hombres (HSH) HIV+. Objetivo: Evaluar frecuencia de infección por HPV-AR, genotipos y lesiones asociadas, y factores asociados. Materiales y métodos: Estudio en HSH HIV+ (septiembre 2012-marzo 2014, Hospital Fernández). Se recogió información demográfica, de HIV, HPV y prácticas sexuales. Se realizó citología anal, detección de HPV-AR (HC2 High- Risk HPV DNA, Digene®) y genotipificación en las muestras HPV-AR+ (Inno Lipa®, Fujirebio). Los pacientes firmaron consentimiento informado. Se indicó tratamiento según resultados. Resultados: Completaron el estudio 57 pacientes. Mediana de edad: 40 años (rango intercuartil [RIC]: 29-45); de CD4: 444 cels/mm3 (RIC: 345-568); 77% recibían tratamiento antirretroviral, 68% con carga viral no detectable. Citologías: negativas (24%); lesión intraepitelial de bajo grado (54%); lesión intraepitelial de alto grado (20%); ASCUS (2%). El 80% fue HPV-AR+. Los pacientes con diagnóstico de HPV-AR (p=0,006) y de lesión intraepitelial tuvieron CD4 <500 cels/ mm3 con más frecuencia (p=0,030). Los pacientes con HPV-AR tuvieron mayor frecuencia de carga viral detectable (p=0,020, prueba de Fisher). El porcentaje de pacientes con uso consistente de preservativo fue mayor entre los pacientes sin lesión citológica (p=0,026). Genotipos de alto riesgo más frecuentes: HPV-16 (51%), HPV-31 (44%) y HPV-51 (40%); de bajo riesgo: HPV-6 (47%) y HPV-44 (35%). Conclusiones: Se encontró elevada frecuencia de lesión citológica (76%) y de HPV-AR (80%). Es necesario establecer estrategias de prevención en esta población incluyendo tamizaje, vacunación y promoción de sexo seguro.

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