Opa Typing of Neisseria gonorrhoeae Strains Isolated From Patients Attending Sexually Transmitted Disease Clinics in China

2007 ◽  
Vol PAP ◽  
Author(s):  
Qiang Chen ◽  
Yue-Ping Yin ◽  
Xiu-Qin Dai ◽  
Yan-Hua Yu ◽  
Hong-Chun Wang ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Sexually Transmitted Disease ◽  
Transmitted Disease ◽  
Sexually Transmitted

scholarly journals Structure of the RecombinantNeisseria gonorrhoeaeAdhesin Complex Protein (rNg-ACP) and Generation of Murine Antibodies with Bactericidal Activity against Gonococci

mSphere ◽  
2018 ◽  
Vol 3 (5) ◽  
Author(s):  
Hannia Liliana Almonacid-Mendoza ◽  
María Victoria Humbert ◽  
Aiste Dijokaite ◽  
David W. Cleary ◽  
Yiwen Soo ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Sexually Transmitted Disease ◽  
Transmitted Disease ◽  
Causative Organism ◽  
Human Lysozyme ◽  
Content Type ◽  
Sexually Transmitted ◽  
Innate Defense ◽  
Complex Protein

ABSTRACTNeisseria gonorrhoeae(gonococcus [Ng]) is the causative organism of the sexually transmitted disease gonorrhoea, and no effective vaccine exists currently. In this study, the structure, biological properties, and vaccine potential of the Ng-adhesin complex protein (Ng-ACP) are presented. The crystal structure of recombinant Ng-ACP (rNg-ACP) protein was solved at 1.65 Å. Diversity and conservation of Ng-ACP were examined in differentNeisseriaspecies and gonococcal isolates (https://pubmlst.org/neisseria/database)in silico, and protein expression among 50 gonococcal strains in the Centers for Disease Control and Prevention/Food and Drug Administration (CDCP/FDA) AR Isolate Bank was examined by Western blotting. Murine antisera were raised to allele 10 (strain P9-17)-encoded rNg-ACP protein with different adjuvants and examined by enzyme-linked immunosorbent assay (ELISA), Western blotting, and a human serum bactericidal assay. Rabbit antiserum to rNg-ACP was tested for its ability to prevent Ng-ACP from inhibiting human lysozyme activityin vitro.Ng-ACP is structurally homologous toNeisseria meningitidisACP and MliC/PliC lysozyme inhibitors. Gonococci expressed predominantly allele 10- and allele 6-encoded Ng-ACP (81% and 15% of isolates, respectively). Murine antisera were bactericidal (titers of 64 to 512,P < 0.05) for the homologous P9-17 strain and heterologous (allele 6) FA1090 strain. Rabbit anti-rNg-ACP serum prevented Ng-ACP from inhibiting human lysozyme with ∼100% efficiency. Ng-ACP protein was expressed by all 50 gonococcal isolates examined with minor differences in the relative levels of expression. rNg-ACP is a potential vaccine candidate that induces antibodies that (i) are bactericidal and (ii) prevent the gonococcus from inhibiting the lytic activity of an innate defense molecule.IMPORTANCENeisseria gonorrhoeae(gonococcus [Ng]) is the causative organism of the sexually transmitted disease gonorrhoea, and the organism is listed by the World Health Organization as a high-priority pathogen for research and development of new control measures, including vaccines. In this study, we demonstrated that theN. gonorrhoeaeadhesin complex protein (Ng-ACP) was conserved and expressed by 50 gonococcal strains and that recombinant proteins induced antibodies in mice that killed the bacteriain vitro. We determined the structure of Ng-ACP by X-ray crystallography and investigated structural conservation withNeisseria meningitidisACP and MliC/PliC proteins from other bacteria which act as inhibitors of the human innate defense molecule lysozyme. These findings are important and suggest that Ng-ACP could provide a potential dual target for tackling gonococcal infections.

Evaluation of a modified enzyme immunoassay for Neisseria gonorrhoeae in high- and low-risk females

1985 ◽  
Vol 31 (10) ◽  
pp. 893-895 ◽  
Author(s):  
Michael R. Skeels ◽  
Bessie Matsuda ◽  
Henry Horton ◽  
James Sampson ◽  
Gary A. Sawyer ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Enzyme Immunoassay ◽  
Sexually Transmitted Disease ◽  
False Positive ◽  
False Negative ◽  
Transmitted Disease ◽  
Low Risk ◽  
Sexually Transmitted ◽  
Disease Clinic ◽  
Sexually Transmitted Disease Clinic

A recently modified commercial enzyme immunoassay (Gonozyme; Abbott Laboratories, North Chicago, IL) for Neisseria gonorrhoeae antigens was compared with bacteriological culture for diagnostic sensitivity, specificity, and predictive value. A total of 480 specimens were tested by both methods; 355 from females attending a sexually transmitted disease clinic ("high-risk") and 125 from female Family Planning clients ("low-risk"). Sensitivity and specificity of enzyme immunoassay for sexually transmitted disease clinic specimens were 79.7 and 97.9%, respectively (55 positive, 280 negative, 6 false positive, 14 false negative). In the low-risk population, sensitivity and specificity were 100 and 97.5%, respectively (3 positive, 119 negative, 3 false positive, 0 false negative). Despite modification, sensitivity of the method remains low for our sexually transmitted disease clinic population, but the test may have applicability for preliminary screening in low-prevalence settings.

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