Rotenone inhibits delayed rectifier K+ current via a protein kinase A-dependent mechanism

Neuroreport ◽  
2008 ◽  
Vol 19 (14) ◽  
pp. 1401-1405 ◽  
Author(s):  
Xiao-Fei Gao ◽  
Wei Wang ◽  
Cheng He
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Delayed Rectifier ◽  
K Current ◽  
Kinase A ◽  
Dependent Mechanism

Amoxapine Inhibits the Delayed Rectifier Outward K+ Current in Mouse Cortical Neurons via cAMP/Protein Kinase A Pathways

2009 ◽  
Vol 332 (2) ◽  
pp. 437-445 ◽  
Author(s):  
Yan-Lin He ◽  
Xiao-Qin Zhan ◽  
Guang Yang ◽  
Ji Sun ◽  
Yan-Ai Mei
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Cortical Neurons ◽  
Delayed Rectifier ◽  
K Current ◽  
Kinase A

scholarly journals Effects of protein phosphatase and kinase inhibitors on the cardiac L-type Ca current suggest two sites are phosphorylated by protein kinase A and another protein kinase.

1995 ◽  
Vol 106 (3) ◽  
pp. 393-414 ◽  
Author(s):  
H C Hartzell ◽  
Y Hirayama ◽  
J Petit-Jacques
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Protein Phosphatase ◽  
Kinase Inhibitors ◽  
Delayed Rectifier ◽  
Internal Perfusion ◽  
Ca Current ◽  
Phosphatase Inhibitors ◽  
Pkc Inhibitors ◽  
Kinase A

We previously showed (Frace, A.M. and H.C. Hartzell. 1993. Journal of Physiology. 472:305-326) that internal perfusion of frog atrial myocytes with the nonselective protein phosphatase inhibitors microcystin or okadaic acid produced an increase in the L-type Ca current (ICa) and a decrease in the delayed rectifier K current (IK). We hypothesized that microcystin revealed the activity of a protein kinase (PKX) that was basally active in the cardiac myocyte that could phosphorylate the Ca and K channels or regulators of the channels. The present studies were aimed at determining the nature of PKX and its phosphorylation target. The effect of internal perfusion with microcystin on ICa or IK was not attenuated by inhibitors of protein kinase A (PKA). However, the effect of microcystin on ICa was largely blocked by the nonselective protein kinase inhibitors staurosporine (10-30 nM), K252a (250 nM), and H-7 (10 microM). Staurosporine and H-7 also decreased the stimulation of ICa by isoproterenol, but K252a was more selective and blocked the ability of microcystin to stimulate ICa without significantly reducing isoproterenol-stimulated current. Internal perfusion with selective inhibitors of protein kinase C (PKC), including the autoinhibitory pseudosubstrate PKC peptide (PKC(19-31)) and a myristoylated derivative of this peptide had no effect. External application of several PKC inhibitors had negative side effects that prevented their use as selective PKC inhibitors. Nevertheless, we conclude that PKX is not PKC. PKA and PKX phosphorylate sites with different sensitivities to the phosphatase inhibitors calyculin A and microcystin. In contrast to the results with ICa, the effect of microcystin on IK was not blocked by any of the kinase inhibitors tested, suggesting that the effect of microcystin on IK may not be mediated by a protein kinase but may be due to a direct effect of microcystin on the IK channel.

A cyclic AMP protein kinase A-dependent mechanism by which rotavirus impairs the expression and enzyme activity of brush border-associated sucrase-isomaltase in differentiated intestinal Caco-2 cells

2004 ◽  
Vol 6 (8) ◽  
pp. 719-731 ◽  
Author(s):  
Sandra Martin-Latil ◽  
Jacqueline Cotte-Laffitte ◽  
Isabelle Beau ◽  
Anne-Marie Quéro ◽  
Monique Géniteau-Legendre ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Protein Kinase A ◽  
Brush Border ◽  
Kinase A ◽  
Dependent Mechanism

Cytokines induce protein kinase A-mediated signalling by a redox-dependent mechanism in rat renal mesangial cells

2015 ◽  
Vol 93 (3) ◽  
pp. 362-369 ◽  
Author(s):  
Karl-Friedrich Beck ◽  
Johannes Euler ◽  
Florian Eisel ◽  
Martina Beck ◽  
Yvette Köhler ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Mesangial Cells ◽  
Kinase A ◽  
Dependent Mechanism

scholarly journals Up-regulation ofpermRNA Expression by Parathyroid Hormone through a Protein Kinase A-CREB-dependent Mechanism in Chondrocytes

2006 ◽  
Vol 281 (33) ◽  
pp. 23632-23642 ◽  
Author(s):  
Eiichi Hinoi ◽  
Taichi Ueshima ◽  
Hironori Hojo ◽  
Mika Iemata ◽  
Takeshi Takarada ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Kinase A ◽  
Dependent Mechanism
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