scholarly journals Low-cost, open-access quantitative phase imaging of algal cells using the transport of intensity equation

2020 ◽  
Vol 7 (1) ◽  
pp. 191921
Author(s):  
Stephen D. Grant ◽  
Kyle Richford ◽  
Heidi L. Burdett ◽  
David McKee ◽  
Brian R. Patton
Keyword(s):  
Biological Samples ◽  
Low Cost ◽  
Algal Species ◽  
Optical Path Length ◽  
Phase Imaging ◽  
Quantitative Phase ◽  
Spatially Resolved ◽  
Potential Applications ◽  
Transport Of Intensity Equation ◽  
Open Source Hardware

Phase microscopy allows stain-free imaging of transparent biological samples. One technique, using the transport of intensity equation (TIE), can be performed without dedicated hardware by simply processing pairs of images taken at known spacings within the sample. The resulting TIE images are quantitative phase maps of unstained biological samples. Therefore, spatially resolved optical path length (OPL) information can also be determined. Using low-cost, open-source hardware, we applied the TIE to living algal cells to measure their effect on OPL. We obtained OPL values that were repeatable within species and differed by distinct amounts depending on the species being measured. We suggest TIE imaging as a method of discrimination between different algal species and, potentially, non-biological materials, based on refractive index/OPL. Potential applications in biogeochemical modelling and climate sciences are suggested.

scholarly journals Low-cost, open-access refractive index mapping of algal cells using the transport of intensity equation

2019 ◽  
Author(s):  
Stephen Grant ◽  
Kyle Richford ◽  
Heidi Burdett ◽  
David McKee ◽  
Brian R. Patton
Keyword(s):  
Refractive Index ◽  
Biological Samples ◽  
Low Cost ◽  
Algal Species ◽  
Spatially Resolved ◽  
Potential Applications ◽  
Index Mapping ◽  
Transport Of Intensity Equation ◽  
Open Source Hardware ◽  
Species Being

AbstractPhase contrast microscopy allows stain free imaging of transparent biological samples. One technique, using the transport of intensity equation (TIE), can be performed without dedicated hardware by simply processing pairs of images taken at known spacings within the sample. The resulting TIE images are quantitative phase maps of unstained biological samples. Therefore, spatially resolved refractive index information can also be determined.Using low-cost, open-source hardware, we applied the TIE to living algal cells to measure their refractive index. We obtained refractive index values that were repeatable within species and differed by distinct amounts depending on the species being measured. We suggest TIE imaging as a method of discrimination between different algal species and, potentially, non-biological materials, based on refractive index. Potential applications in biogeochemical modelling and climate sciences are suggested.

scholarly journals Tissue disorder strength measured by quantitative phase imaging as intrinsic cancer marker

2017 ◽  
Author(s):  
Masanori Takabayashi ◽  
Hassaan Majeed ◽  
Andre Kajdacsy-Balla ◽  
Gabriel Popescu
Keyword(s):  
Refractive Index ◽  
Imaging System ◽  
Tissue Sample ◽  
Optical Path Length ◽  
Phase Imaging ◽  
Disorder Strength ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Path Length Difference ◽  
Malignant Breast

AbstractTissue refractive index provides important information about morphology at the nanoscale. Since the malignant transformation involves both intra- and inter-cellular changes in the refractive index map, the tissue disorder measurement can be used to extract important diagnosis information. Quantitative phase imaging (QPI) provides a practical means of extracting this information as it maps the optical path-length difference (OPD) across a tissue sample with sub-wavelength sensitivity. In this work, we employ QPI to compare the tissue disorder strength between benign and malignant breast tissue histology samples. Our results show that disease progression is marked by a significant increase in the disorder strength. Since our imaging system can be added as an upgrading module to an existing microscope, we anticipate that it can be integrated easily in the pathology work flow.

Research Progress of Paper-Based Microfluidic Devices

2013 ◽  
Vol 421 ◽  
pp. 334-336 ◽  
Author(s):  
Yong Qiang Cheng ◽  
Cui Lian Guo ◽  
Yang Li ◽  
Bin Zhao ◽  
Xiao Cui
Keyword(s):  
Environmental Monitoring ◽  
Plasma Etching ◽  
Biological Samples ◽  
Recent Progress ◽  
Point Of Care ◽  
Low Cost ◽  
Microfluidic Devices ◽  
Research Progress ◽  
Potential Applications

Paper-based microfluidic devices have recently received increasing attention as a potential platform for its low cost, portability and excellent compatibility with biological samples. A variety of fabrication technologies were employed, including simple photolithography, wax plotting, printing, inkjet etching, plasma etching and so on. Meanwhile, the potential applications of paper-based microfluidic devices in diagnostic, point-of-care (POC), and environmental monitoring were reported. We review the recent progress of fabrication technologies and the applications of paper-based microfluidic devices.

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