scholarly journals Fingerprinting taste buds: intermediate filaments and their implication for taste bud formation

2000 ◽  
Vol 355 (1401) ◽  
pp. 1233-1237 ◽  
Author(s):  
Martin Witt ◽  
Klaus Reutter ◽  
Donald Ganchrow ◽  
Judith R. Ganchrow
Keyword(s):  
Epithelial Cells ◽  
Intermediate Filaments ◽  
Taste Buds ◽  
Taste Bud ◽  
Intermediate Filament Protein ◽  
Cytokeratin 20 ◽  
Nerve Fibres ◽  
Vimentin Expression ◽  
Disc Cells ◽  
Taste Bud Cells

Intermediate filaments in taste organs of terrestrial (human and chick) as well as aquatic ( Xenopus laevis ) species were detected using immunohistochemistry and electron microscopy. During development, the potential importance of the interface between the taste bud primordium and non–gustatory, adjacent tissues is evidenced by the distinct immunoreactivity of a subpopulation of taste bud cells for cytokeratins and vimentin. In human foetuses, the selective molecular marker for taste bud primordia, cytokeratin 20, is not detectable prior to the ingrowth of nerve fibres into the epithelium, which supports the hypothesis that nerve fibres are necessary for initiating taste bud development. Another intermediate filament protein, vimentin, occurs in derivatives of mesoderm, but usually not in epithelium. In humans, vimentin immunoreactivity is expressed mainly in border (marginal) epithelial cells of taste bud primordia, while in chick, vimentin expression occurs in most taste bud cells, whereas non–gustatory epithelium is vimentin immunonegative. Our chick data suggest a relationship between the degree of vimentin expression and taste bud cell proliferation especially during the perihatching period. It is suggested that surrounding epithelial cells (human) and mesenchymal cells (chick) may be contributing sources of developing taste buds. The dense perinuclear network of intermediate filaments especially in dark (i.e. non–sensory) taste disc cells of Xenopus indicates that vimentin filaments also might be associated with cells of non–gustatory function. These results indicate that the mechanisms of taste bud differentiation from source tissues may differ among vertebrates of different taxa.

Some Observations on Changes in Denervated Taste Buds of Circumvallate Papillae of Rabbits

1969 ◽  
Vol 27 ◽  
pp. 308-309
Author(s):  
Sunao Fujimoto ◽  
Raymond G. Murray ◽  
Assia Murray
Keyword(s):  
Taste Buds ◽  
Taste Bud ◽  
Cell Type ◽  
Dark Cells ◽  
Type Iii ◽  
Circumvallate Papillae ◽  
Taste Bud Cells ◽  
Light Cells

Taste bud cells in circumvallate papillae of rabbit have been classified into three groups: dark cells; light cells; and type III cells. Unilateral section of the 9th nerve distal to the petrosal ganglion was performed in 18 animals, and changes of each cell type in the denervated buds were observed from 6 hours to 10 days after the operation.Degeneration of nerves is evident at 12 hours (Fig. 1) and by 2 days, nerves are completely lacking in the buds. Invasion by leucocytes into the buds is remarkable from 6 to 12 hours but then decreases. Their extrusion through the pore is seen. Shrinkage and disturbance in arrangement of cells in the buds can be seen at 2 days. Degenerated buds consisting of a few irregular cells and remnants of degenerated cells are present at 4 days, but buds apparently normal except for the loss of nerve elements are still present at 6 days.

scholarly journals Jacalin and peanut agglutinin (PNA) bindings in the taste bud cells of the rat: New reliable markers for type IV cells of the rat taste buds

2005 ◽  
Vol 68 (4) ◽  
pp. 243-250 ◽  
Author(s):  
Ryo Taniguchi ◽  
Lei Shi ◽  
Masae Fujii ◽  
Katsura Ueda ◽  
Shiho Honma ◽  
...  
Keyword(s):  
Taste Buds ◽  
Taste Bud ◽  
Peanut Agglutinin ◽  
Type Iv ◽  
Taste Bud Cells

scholarly journals SOX2 regulates homeostasis of taste bud cells and lingual epithelial cells in posterior tongue

PLoS ONE ◽  
2020 ◽  
Vol 15 (10) ◽  
pp. e0240848
Author(s):  
Makoto Ohmoto ◽  
Weiwei Lei ◽  
Junpei Yamashita ◽  
Junji Hirota ◽  
Peihua Jiang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Taste Bud ◽  
Posterior Tongue ◽  
Taste Bud Cells

Scanning Electron Microscopy of the Taste Bud of the Mudpuppy, Necturus Maculosus

1975 ◽  
Vol 33 ◽  
pp. 530-531
Author(s):  
David W. Samanen ◽  
Rudy A. Bernard
Keyword(s):  
Taste Buds ◽  
Taste Bud ◽  
General Distribution ◽  
Surface Irregularity ◽  
Lingual Papillae ◽  
Necturus Maculosus ◽  
Neurophysiological Studies ◽  
Taste Bud Cells ◽  
Sectioned Tissue ◽  
Stimulation Of

The tongue of the mudpuppy, Necturus maculosus, appears smooth and without any papillae. Farbman and Yonkers (1971) reported that the tongue contains round elevations or eminences, each with a single, large taste bud. Furthermore, their light micrographs, made of sectioned tissue, showed that the tips of the buds are flush to the lingual surface. In contrast, the mammalian taste buds lie below the epithelium of the lingual papillae and contact the surface only by way of a narrow taste pore. We undertook SEM studies to confirm this morphology, one which would be advantageous for later neurophysiological studies involving the stimulation of individual taste buds and microelectrode recording from taste bud cells.Figure 1 shows two adjacent eminences from the mudpuppy's distal tongue. The taste bud shows as a surface irregularity, centered at the top of each mound. Their dimensions and general distribution correspond to those reported by Farbman and Yonkers.

scholarly journals Ecto-calcium-dependent ATPase activity of mammalian taste bud cells.

1992 ◽  
Vol 40 (12) ◽  
pp. 1919-1928 ◽  
Author(s):  
M A Barry
Keyword(s):  
Plasma Membranes ◽  
Taste Buds ◽  
Taste Bud ◽  
Basal Cells ◽  
Calcium Dependent ◽  
The Core ◽  
Dependent Atpase ◽  
Peripheral Cells ◽  
External Face ◽  
Taste Bud Cells

Histochemistry was utilized to characterize Ca-ATPases associated with lingual taste buds in the golden hamster. Taste buds showed elevated staining for magnesium- or calcium-dependent ATPase (Ca-ATPase) relative to the surrounding epithelium. At low calcium concentrations (0.1-0.5 mM), intracellular staining predominated. Most of the studies were conducted at calcium concentrations of > or = 10 mM, in which most of the staining was localized to the external face of plasma membranes of taste bud cells (including receptor and basal cells) located in the core of fungiform taste buds, or the entire vallate or foliate taste buds. The peripheral fungiform taste bud cells stained much less intensely, but the peripheral cells adjacent to the core showed intermediate levels. GTP and ITP were just as effective substrates as ATP. Millimolar concentrations of magnesium were as effective as calcium. Inhibitors of intracellular ATPases, including quercetin, sodium azide, and 2,4-dinitrophenol, had no effect on the staining. Therefore, the Ca-ATPase staining of plasma membranes at mM concentrations of calcium is thought to correspond to one or more ecto-Ca-ATPase activities with unknown functions. Roles related to increased energy requirements or to the possible function of ATP as a neurotransmitter or -modulator are proposed.

Fine structure of degenerating taste buds after denervation

Development ◽  
1969 ◽  
Vol 22 (1) ◽  
pp. 55-68
Author(s):  
Albert I. Farbman
Keyword(s):  
Cell Death ◽  
Fine Structure ◽  
Taste Buds ◽  
Taste Bud ◽  
Nerve Terminals ◽  
Nerve Supply ◽  
Cell Turnover ◽  
Epithelial Surface ◽  
Taste Bud Cells

It is well known that taste buds are dependent on an intact nerve supply, and when experimentally denervated they degenerate and disappear (von Vintschgau & Honigschmied, 1877; von Vintschgau, 1880; Griffini, 1887; Meyer, 1897; Olmsted, 1920a, b, 1921, 1922; May, 1925; Whiteside, 1927; Torrey, 1934, 1936; Wagner, 1953; Guth, 1957, 1958, 1963; Beidler, 1962, 1963). Olmsted (1920b) has suggested that the degenerating taste bud cells are cleared away by macrophages invading the epithelium; Guth (1957, 1958, 1963) has demonstrated sloughing of degenerating taste buds from the epithelial surface, and others believe that taste bud cells dedifferentiate to become lining epithelium (Meyer, 1897; Wagner, 1953). Because of this disagreement and because recent evidence for cell turnover in taste buds has indicated that cell death and replacement is a normal occurrence (Beidler, 1962, 1963; DeLorenzo, 1963; Beidler & Smallman, 1965), it is pertinent to study the fine structure of degenerating taste buds in the hope of elucidating the process by which taste bud cells and nerve terminals degenerate.

Intranuclear Crystals in Epithelial Cells of Perca Flavescens

1977 ◽  
Vol 35 ◽  
pp. 404-405
Author(s):  
Richard L. Leino
Keyword(s):  
Epithelial Cells ◽  
Taste Bud ◽  
Chloride Cells ◽  
Nuclear Inclusions ◽  
Eosinophilic Inclusion ◽  
Minnesota Lakes ◽  
Columnar Cells ◽  
Rodlet Cells ◽  
Taste Bud Cells ◽  
Electron Lucent

During microscopic examination of tissues of healthy-appearing perch caught in N.E. Minnesota lakes, it was noticed that the specimens frequently had variable numbers of epithelial cells whose nuclei contained eosinophilic inclusion bodies. These nuclear inclusions were found in a number of sites: surface epithelial cells of gill, skin and distal rectum; columnar cells of kidney collecting ducts; taste bud cells and, rarely, chloride cells of gill. In addition, the inclusions were found in rodlet cells in all of these regions, but not in rodlet or other epithelial cells of intestine. Under the electron microscope, these nuclear inclusions appear as 4-, 5-, and 6-sided paracrystals ranging in length from less than 0.5 μm to greater than 3 μm. The inclusions are usually surrounded by a narrow rim of electron-lucent nucleoplasm. Some crystals are composed of circular subunits hooked together (?) in parallel rows.

scholarly journals Insulin Is Transcribed and Translated in Mammalian Taste Bud Cells

Endocrinology ◽  
2018 ◽  
Vol 159 (9) ◽  
pp. 3331-3339 ◽  
Author(s):  
Máire E Doyle ◽  
Jennifer L Fiori ◽  
Isabel Gonzalez Mariscal ◽  
Qing-Rong Liu ◽  
Erin Goodstein ◽  
...  
Keyword(s):  
Green Fluorescence Protein ◽  
Taste Buds ◽  
Taste Bud ◽  
Lingual Epithelium ◽  
Processing Enzymes ◽  
Taste Cells ◽  
Specific Proteins ◽  
Fluorescence Protein ◽  
Taste Bud Cells

Abstract We and others have reported that taste cells in taste buds express many peptides in common with cells in the gut and islets of Langerhans in the pancreas. Islets and taste bud cells express the hormones glucagon and ghrelin, the same ATP-sensitive potassium channel responsible for depolarizing the insulin-secreting β cell during glucose-induced insulin secretion, as well as the propeptide-processing enzymes PC1/3 and PC2. Given the common expression of functionally specific proteins in taste buds and islets, it is surprising that no one has investigated whether insulin is synthesized in taste bud cells. Using immunofluorescence, we demonstrated the presence of insulin in mouse, rat, and human taste bud cells. By detecting the postprocessing insulin molecule C-peptide and green fluorescence protein (GFP) in taste cells of both insulin 1-GFP and insulin 2-GFP mice and the presence of the mouse insulin transcript by in situ hybridization, we further proved that insulin is synthesized in individual taste buds and not taken up from the parenchyma. In addition to our cytology data, we measured the level of insulin transcript by quantitative RT-PCR in the anterior and posterior lingual epithelia. These analyses showed that insulin is translated in the circumvallate and foliate papillae in the posterior, but only insulin transcript was detected in the anterior fungiform papillae of the rodent tongue. Thus, some taste cells are insulin-synthesizing cells generated from a continually replenished source of precursor cells in the adult mammalian lingual epithelium.

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