scholarly journals Immunological Characterization of a Mammary Tumour Virus from Swiss Mice: Multiple Epitopes Associated with the Viral Gene Products

1981 ◽  
Vol 57 (1) ◽  
pp. 75-83 ◽  
Author(s):  
C-M. Calberg-Bacq ◽  
C. Francois ◽  
S. Kozma ◽  
P. M. Osterrieth ◽  
Y. A. Teramoto
Keyword(s):  
Mammary Tumour ◽  
Viral Gene ◽  
Gene Products ◽  
Immunological Characterization ◽  
Swiss Mice

Immunological characterization of human acid phosphatase gene products

1985 ◽  
Vol 23 (3-4) ◽  
pp. 309-319 ◽  
Author(s):  
Abdul Waheed ◽  
Robert L. Van Etten ◽  
V. Gieselmann ◽  
K. von Figura
Keyword(s):  
Acid Phosphatase ◽  
Gene Products ◽  
Immunological Characterization ◽  
Human Acid ◽  
Acid Phosphatase Gene

scholarly journals ISOLATION AND CHARACTERIZATION OF C-TYPE VIRAL GENE PRODUCTS OF VIRUS-NEGATIVE MOUSE CELLS

1974 ◽  
Vol 139 (2) ◽  
pp. 427-438 ◽  
Author(s):  
John R. Stephenson ◽  
Steven R. Tronick ◽  
Roberta K. Reynolds ◽  
Stuart A. Aaronson
Keyword(s):  
Viral Gene ◽  
Gene Products ◽  
New Class ◽  
Viral Antigens ◽  
Isolation And Characterization ◽  
Cell Extracts ◽  
Mouse Cells ◽  
Specific Antigens ◽  
Low Levels

Antigens which immunologically cross-react with two mouse C-type viral polypeptides, p30 and p12, are present at very low levels in normal virus-negative mouse cells. These two antigens have been purified by 50–300-fold from cell extracts and shown to cochromatograph with the corresponding labeled viral polypeptides in several systems. Their type-specific antigenicities are shown to be distinct from those of previously tested MuLV isolates suggesting that they may be components of a new class of endogenous C-type virus. The methods utilized in the present studies for concentration of virus-specific antigens of normal mouse cells provide an approach for detection of C-type viral antigens in cells of other species.

scholarly journals Immunological characterization of the gag gene products of bovine immunodeficiency virus.

1992 ◽  
Vol 66 (12) ◽  
pp. 6868-6877 ◽  
Author(s):  
J K Battles ◽  
M Y Hu ◽  
L Rasmussen ◽  
G J Tobin ◽  
M A Gonda
Keyword(s):  
Bovine Immunodeficiency Virus ◽  
Gene Products ◽  
Immunological Characterization ◽  
Immunodeficiency Virus

Identification of a baculovirus polyhedron formation mutant with a novel phenotype

1996 ◽  
Vol 54 ◽  
pp. 796-797
Author(s):  
James M. Slavicek ◽  
Melissa J. Mercer ◽  
Mary Ellen Kelly
Keyword(s):  
Viral Gene ◽  
Gene Products ◽  
Type Number ◽  
Infection Cycle ◽  
Wild Type ◽  
Protein Matrix ◽  
Insect Midgut ◽  
Viral Particles ◽  
Budded Virus ◽  
Viral Form

Nucleopolyhedroviruses (NPV, family Baculoviridae) produce two morphological forms, a budded virus form and a viral form that is occluded into a paracrystalline protein matrix. This structure is termed a polyhedron and is composed primarily of the protein polyhedrin. Insects are infected by NPVs after ingestion of the polyhedron and release of the occluded virions through dissolution of the polyhedron in the alkaline environment of the insect midgut. Early after infection the budded virus form is produced. It buds through the plasma membrane and then infects other cells. Later in the infection cycle the occluded form of the virus is generated (reviewed by Blissard and Rohrmann, 1990).The processes of polyhedron formation and virion occlusion are likely to involve a number of viral gene products. However, only two genes, the polyhedrin gene and 25K FP gene, have been identified to date that are necessary for the wild type number of polyhedra to be formed and viral particles occluded.

The Immunological Characterization of Human Antibodies to Factor VIII Isolated by Immuno-Affinity Chromatography

1981 ◽  
Vol 45 (01) ◽  
pp. 060-064 ◽  
Author(s):  
M L Kavanagh ◽  
C N Wood ◽  
J F Davidson
Keyword(s):  
Factor Viii ◽  
Affinity Chromatography ◽  
Gel Filtration ◽  
Immunological Characterization ◽  
Human Antibodies ◽  
Heavy Chains ◽  
Light Chain Type ◽  
Antibody Preparations ◽  
Chain Type

SummaryNine human antibodies to factor VIII were isolated from haemophilic plasmas by affinity chromatography and gel filtration and six were subsequently subjected to immunological characterization. Three partially purified preparations were similarly characterized. Eight of the antibodies were characterized as being exclusively IgG and one preparation was found to contain IgM. Seven of the antibodies contained only a single light chain type, four being of type lambda and three of type kappa. Two antibody preparations contained both kappa and lambda light chains. In four of the preparations, only a single heavy chain sub-class could be demonstrated, three of IgG3 and one of IgG4. Of the remainder, three were a mixture of IgG3 and IgG4 sub-classes and one contained both IgG2 and IgG4. IgG sub-classification could not be achieved with the IgM-containing preparation. These results demonstrate a restricted heterogeneity of light and heavy chains in human antibodies to factor VIII.

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