Detection of the movement protein of red clover necrotic mosaic virus in a cell wall fraction from infected Nicotiana clevelandii plants

Cell–to–cell movement of tobacco mosaic virus (TMV) is used to illustrate macromolecular traffic through plant intercellular connections, the plasmodesmata. This transport process is mediated by a specialized viral movement protein, P30. In the initially infected cell, P30 is produced by transcription of a subgenomic RNA derived from the invading virus. Presumably, P30 then associates with a certain proportion of the viral RNA molecules, sequestering them from replication and mediating their transport into neighbouring uninfected host cells. This nucleoprotein complex is targeted to plasmodesmata, possibly via interaction with the host cell cytoskeleton. Prior to passage through a plasmodesma, the plasmodesmal channel is dilated by the movement protein. It is proposed that targeting of P30–TMV RNA complexes to plasmodesmata involves binding to a specific cell wall–associated receptor molecule. In addition, a cell wall–associated protein kinase, phosphorylates P30 at its carboxy–terminus and minimizes P30–induced interference with plasmodesmatal permeability during viral infection.

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Viral cell-to-cell movement requires formation of cortical punctate structures containing Red clover necrotic mosaic virus movement protein

Virology ◽

10.1016/j.virol.2011.02.008 ◽

2011 ◽

Vol 413 (2) ◽

pp. 205-215 ◽

Cited By ~ 24

Author(s):

Masanori Kaido ◽

Naoko Funatsu ◽

Yasuko Tsuno ◽

Kazuyuki Mise ◽

Tetsuro Okuno

Keyword(s):

Mosaic Virus ◽

Movement Protein ◽

Cell Movement ◽

Red Clover ◽

Virus Movement

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Cell-Associated Pheromone Peptide (cCF10) Production and Pheromone Inhibition in Enterococcus faecalis

Journal of Bacteriology ◽

10.1128/jb.182.17.4926-4933.2000 ◽

2000 ◽

Vol 182 (17) ◽

pp. 4926-4933 ◽

Cited By ~ 49

Author(s):

B. A. (Leonard) Buttaro ◽

M. H. Antiporta ◽

G. M. Dunny

Keyword(s):

Cell Wall ◽

Protease Inhibitor ◽

Enterococcus Faecalis ◽

Cell Envelope ◽

Conjugative Plasmid ◽

Cell Fractionation ◽

Cell Wall Fraction ◽

Donor Cells ◽

Pheromone Activity ◽

A Cell

ABSTRACT In Enterococcus faecalis, the peptide cCF10 acts as a pheromone, inducing transfer of the conjugative plasmid pCF10 from plasmid-containing donor cells to plasmid-free recipient cells. In these studies, it was found that a substantial amount of cCF10 associates with the envelope of the producing cell. Pheromone activity was detected in both wall and membrane fractions, with the highest activity associated with the wall. Experiments examining the effects of protease inhibitor treatments either prior to or following cell fractionation suggested the presence of a cell envelope-associated pro-cCF10 that can be processed to mature cCF10 by a maturase or protease. A pCF10-encoded membrane protein, PrgY, was shown to prevent self-induction of donor cells by reducing the level of pheromone activity in the cell wall fraction.

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