scholarly journals Two key mutations in the host-range specificity domain of the p143 gene of Autographa californica nucleopolyhedrovirus are required to kill Bombyx mori larvae.

1998 ◽  
Vol 79 (4) ◽  
pp. 931-935 ◽  
Author(s):  
O Argaud ◽  
M L√≥pez-Ferber ◽  
L Croizier ◽  
G Croizier
Keyword(s):  
Host Range ◽  
Bombyx Mori ◽  
Autographa Californica ◽  
Autographa Californica Nucleopolyhedrovirus ◽  
Specificity Domain

scholarly journals A baculovirus isolated from wild silkworm encompasses the host ranges of Bombyx mori nucleopolyhedrosis virus and Autographa californica multiple nucleopolyhedrovirus in cultured cells

2012 ◽  
Vol 93 (11) ◽  
pp. 2480-2489 ◽  
Author(s):  
Yi-Peng Xu ◽  
Lin-Zhu Gu ◽  
Yi-Han Lou ◽  
Ruo-Lin Cheng ◽  
Hai-Jun Xu ◽  
...  
Keyword(s):  
Host Range ◽  
Bombyx Mori ◽  
Genome Sequence ◽  
Trichoplusia Ni ◽  
Cultured Cells ◽  
Autographa Californica ◽  
Multiple Nucleopolyhedrovirus ◽  
Comparison Results ◽  
Wild Silkworm ◽  
Nucleopolyhedrosis Virus

A baculovirus, named BomaNPV S2, was isolated from a diseased larva of the wild silkworm, Bombyx mandarina. Notably, BomaNPV S2 exhibited a distinguishing feature in that its host range covered that of both Bombyx mori nucleopolyhedrosis virus (BmNPV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in cultured cells. It could replicate in cells of B. mori (Bm5 and BmN), Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn-5B1-4). However, occlusion-derived virions of BomaNPV S2 in B. mori cells contained only a single nucleocapsid, whereas they contained multiple nucleocapsids in Tn-5B1-4 cells. The complete genome sequence of BomaNPV S2, including predicted ORFs, was determined and compared with the genome sequence of its close relatives. The comparison results showed that most of the BomaNPV S2 genome sequence was shared with BmNPV (BmNPV T3) or BomaNPV S1, but several regions seemed more similar to regions of AcMNPV. This observation might explain why BomaNPV S2 covers the host ranges of BmNPV and AcMNPV. Further recombinant virus infection experiments demonstrated that GP64 plays an important role in BomaNPV S2 host-range determination.

scholarly journals Lymantria dispar Nucleopolyhedrovirushrf-1 Expands the Larval Host Range of Autographa californica Nucleopolyhedrovirus

1998 ◽  
Vol 72 (3) ◽  
pp. 2526-2531 ◽  
Author(s):  
Chi-Ju Chen ◽  
Martha E. Quentin ◽  
Lynn A. Brennan ◽  
Christine Kukel ◽  
Suzanne M. Thiem
Keyword(s):  
Host Range ◽  
Lymantria Dispar ◽  
Helicoverpa Zea ◽  
Autographa Californica ◽  
Insect Larvae ◽  
Resistant Species ◽  
Larval Host ◽  
Autographa Californica Nucleopolyhedrovirus ◽  
High Doses ◽  
Third Instar Larvae

The gypsy moth (Lymantria dispar) is nonpermissive forAutographa californica nucleopolyhedrovirus (AcNPV) infection. We previously isolated a gene, host range factor 1 (hrf-1), from L. dispar nucleopolyhedrovirus that promotes AcNPV replication in Ld652Y cells, a nonpermissiveL. dispar cell line (S. M. Thiem, X. Du, M. E. Quentin, and M. M. Berner, J. Virol. 70:2221–2229, 1996). In the present study, we investigated the ability of hrf-1 to alter the larval host range of AcNPV. Bioassays using recombinant AcNPV bearing hrf-1 were conducted with insect larvae by use of oral infection. AcNPV bearing hrf-1 was infectious for neonate L. dispar larvae, with a 50% lethal concentration of 1.2 × 105 polyhedral inclusion bodies/ml of diet, which is similar to that of wild-type AcNPV for permissive hosts. AcNPV can kill neonate L. dispar larvae at high doses, but it does not kill third-instar larvae. However, electron microscopy studies of AcNPV-inoculated third-instar larvae revealed virus replication in the midgut cells. PCR analyses indicated that the virus was AcNPV. These results suggest that the block for AcNPV infection of L. dispar larvae is its inability to spread systematically from primary infection sites in the midgut epithelium and that this barrier is leaky in neonates. hrf-1 allows AcNPV to overcome this barrier. AcNPV recombinants bearing hrf-1 were also significantly more infectious for Helicoverpa zea, a resistant species, suggesting that the blocks for AcNPV infection ofL. dispar and H. zea larvae may be similar.

scholarly journals Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Hemolymph in Response to Autographa californica Nucleopolyhedrovirus in Differentially Resistant Strains

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1401
Author(s):  
Xin-yi Ding ◽  
Xue-yang Wang ◽  
Yun-hui Kong ◽  
Chun-xiao Zhao ◽  
Sheng Qin ◽  
...  
Keyword(s):  
Viral Infection ◽  
Bombyx Mori ◽  
Metabolic Pathways ◽  
Autographa Californica ◽  
Economic Losses ◽  
Viral Gene ◽  
Apoptosis Pathway ◽  
Larval Hemolymph ◽  
Autographa Californica Nucleopolyhedrovirus ◽  
Resistant Strains

Bombyx mori nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although Autographa californica nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of BmTex261 in viral infection was analysed. The BmTex261 showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene lef3 after overexpression of BmTex261 in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms.

scholarly journals In vitro host range of Autographa californica nucleopolyhedrovirus recombinants lacking functional p35, iap1 or iap2.

1999 ◽  
Vol 80 (4) ◽  
pp. 1055-1066 ◽  
Author(s):  
C M Griffiths ◽  
A L Barnett ◽  
M D Ayres ◽  
J Windass ◽  
L A King ◽  
...  
Keyword(s):  
Host Range ◽  
Autographa Californica ◽  
Autographa Californica Nucleopolyhedrovirus

scholarly journals A MicroRNA Encoded by Autographa californica Nucleopolyhedrovirus Regulates Expression of Viral Gene ODV-E25

2013 ◽  
Vol 87 (23) ◽  
pp. 13029-13034 ◽  
Author(s):  
Mengxiao Zhu ◽  
Jinwen Wang ◽  
Riqiang Deng ◽  
Peiwen Xiong ◽  
Hai Liang ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Bombyx Mori ◽  
Autographa Californica ◽  
Viral Gene ◽  
Content Type ◽  
Coding Sequence ◽  
Autographa Californica Nucleopolyhedrovirus ◽  
Identification And Characterization

Baculovirus-encoded microRNAs (miRNAs) have been described inBombyx morinucleopolyhedrovirus; however, most of their functions remain unclear. Here we report the identification and characterization of an miRNA encoded byAutographa californicanucleopolyhedrovirus. The identified miRNA, AcMNPV-miR-1, perfectly matched a segment in the coding sequence of the viral gene ODV-E25 and downregulated ODV-E25 mRNA expression, which likely resulted in a reduction of infectious budded virions and accelerated the formation of occlusion-derived virions.

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