scholarly journals Analysis of the complete genome sequence of black queen-cell virus, a picorna-like virus of honey bees

2000 ◽  
Vol 81 (8) ◽  
pp. 2111-2119 ◽  
Author(s):  
Neil Leat ◽  
Brenda Ball ◽  
Vandana Govan ◽  
Sean Davison
Keyword(s):  
South African ◽  
Honey Bees ◽  
Untranslated Region ◽  
Rhopalosiphum Padi ◽  
Amino Acid Sequences ◽  
Nucleotide Sequence Analysis ◽  
Black Queen Cell Virus ◽  
Queen Cell ◽  
African Honey Bees ◽  
Plautia Stali

A virus with picorna-like biophysical properties was isolated from South African honey bees. On the basis of serology, it was identified as an isolate of black queen-cell virus (BQCV). Nucleotide sequence analysis revealed an 8550 nt polyadenylated genome containing two large ORFs. The 5′-proximal ORF (ORF 1) represented 4968 nt while the 3′-proximal ORF (ORF 2) represented 2562 nt. The ORFs were separated by a 208 nt intergenic region and were flanked by a 657 nt 5′-untranslated region and a 155 nt 3′-untranslated region. Deduced amino acid sequences for ORF 1 and ORF 2 were most similar to the non-structural and structural proteins, respectively, of Drosophila C virus (DCV), Rhopalosiphum padi virus (RhPV), Himetobi P virus (HiPV) and Plautia stali intestine virus (PSIV). It is proposed that BQCV belongs to the group of picorna-like, insect-infecting RNA viruses constituted by DCV, RhPV, HiPV and PSIV.

scholarly journals Brain transcriptomes of honey bees ( Apis mellifera ) experimentally infected by two pathogens: Black queen cell virus and Nosema ceranae

Genomics Data ◽  
2016 ◽  
Vol 10 ◽  
pp. 79-82 ◽  
Author(s):  
Vincent Doublet ◽  
Robert J. Paxton ◽  
Cynthia M. McDonnell ◽  
Emeric Dubois ◽  
Sabine Nidelet ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Nosema Ceranae ◽  
Black Queen Cell Virus ◽  
Queen Cell

scholarly journals Development of infectious transcripts and genome manipulation of Black queen-cell virus of honey bees

2002 ◽  
Vol 83 (12) ◽  
pp. 3139-3146 ◽  
Author(s):  
Mongi Benjeddou ◽  
Neil Leat ◽  
Mike Allsopp ◽  
Sean Davison
Keyword(s):  
South African ◽  
Restriction Site ◽  
Promoter Sequence ◽  
Wild Type Virus ◽  
Black Queen Cell Virus ◽  
South African Isolate ◽  
Viral Particles ◽  
Queen Cell ◽  
Antisense Primer

The South African isolate of Black queen-cell virus (BQCV), a honey bee virus, was previously found to have an 8550 nucleotide genome excluding the poly(A) tail. Its genome contained two ORFs, a 5′-proximal ORF encoding a putative replicase protein and a 3′-proximal ORF encoding a capsid polyprotein. Long reverse transcription (RT)–PCR was used to produce infectious transcripts for BQCV and to manipulate its genome. Primers were designed for the amplification of the complete genome, the in vitro transcription of infectious RNA and PCR-directed mutagenesis. An 18-mer antisense primer was designed for RT to produce full-length single-stranded cDNA (ss cDNA). Unpurified ss cDNA from the RT reaction mixture was used directly as a template to amplify the full genome by long high-fidelity PCR. The SP6 promoter sequence was introduced into the sense primer to transcribe RNA directly from the amplicon. RNA was transcribed in vitro with and without the presence of a cap analogue and injected directly into bee pupae, which were then incubated for 8 days. In vitro transcripts were infectious but the presence of a cap analogue did not increase the amount of virus recovered. A single base mutation abolishing an EcoRI restriction site was introduced by fusion-PCR, to distinguish viral particles recovered from infectious transcripts from wild-type virus (wtBQCV). Mutant virus (mutBQCV) and wtBQCV were indistinguishable by electron microscopy and Western blot analysis. The EcoRI restriction site was present in wtBQCV and not in mutBQCV.

scholarly journals Mode of Transmission Determines the Virulence of Black Queen Cell Virus in Adult Honey Bees, Posing a Future Threat to Bees and Apiculture

Viruses ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 535 ◽  
Author(s):  
Yahya Al Naggar ◽  
Robert J. Paxton
Keyword(s):  
Honey Bees ◽  
Horizontal Transmission ◽  
Well Being ◽  
Rnai Pathway ◽  
Viral Titer ◽  
Black Queen Cell Virus ◽  
Viral Pathogen ◽  
Viral Virulence ◽  
Queen Cell ◽  
Mode Of Transmission

Honey bees (Apis mellifera) can be infected by many viruses, some of which pose a major threat to their health and well-being. A critical step in the dynamics of a viral infection is its mode of transmission. Here, we compared for the first time the effect of mode of horizontal transmission of Black queen cell virus (BQCV), a ubiquitous and highly prevalent virus of A. mellifera, on viral virulence in individual adult honey bees. Hosts were exposed to BQCV either by feeding (representing direct transmission) or by injection into hemolymph (analogous to indirect or vector-mediated transmission) through a controlled laboratory experimental design. Mortality, viral titer and expression of three key innate immune-related genes were then quantified. Injecting BQCV directly into hemolymph in the hemocoel resulted in far higher mortality as well as increased viral titer and significant change in the expression of key components of the RNAi pathway compared to feeding honey bees BQCV. Our results support the hypothesis that mode of horizontal transmission determines BQCV virulence in honey bees. BQCV is currently considered a benign viral pathogen of adult honey bees, possibly because its mode of horizontal transmission is primarily direct, per os. We anticipate adverse health effects on honey bees if BQCV transmission becomes vector-mediated.

scholarly journals Coding-Complete Genome Sequence of a Black Queen Cell Virus Isolate from Honey Bees (Apis mellifera) in Italy

2020 ◽  
Vol 9 (28) ◽  
Author(s):  
Raied Abou Kubaa ◽  
Annalisa Giampetruzzi ◽  
Rocco Addante ◽  
Maria Saponari
Keyword(s):  
Apis Mellifera ◽  
Genome Sequence ◽  
Honey Bees ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virus Isolate ◽  
High Similarity ◽  
Black Queen Cell Virus ◽  
Queen Cell ◽  
Genome Phylogeny

ABSTRACT In this study, we documented the complete coding genome sequence of a Black queen cell virus (BQCV) isolate from honey bees in Italy. This genome sequence illustrates a high similarity with other BQCV isolates reported worldwide and could provide insights into BQCV genome phylogeny and divergence.

scholarly journals Viral transmission in honey bees and native bees, supported by a global black queen cell virus phylogeny

2019 ◽  
Vol 21 (3) ◽  
pp. 972-983 ◽  
Author(s):  
Elizabeth A. Murray ◽  
John Burand ◽  
Natalia Trikoz ◽  
Julia Schnabel ◽  
Heather Grab ◽  
...  
Keyword(s):  
Honey Bees ◽  
Viral Transmission ◽  
Native Bees ◽  
Black Queen Cell Virus ◽  
Queen Cell

A scientific note on the first detection of black queen cell virus in honey bees (Apis mellifera) in Mexico

Apidologie ◽  
2013 ◽  
Vol 44 (4) ◽  
pp. 382-384 ◽  
Author(s):  
Ernesto Guzman-Novoa ◽  
Mollah Md. Hamiduzzaman ◽  
Adriana Correa-Benítez ◽  
Laura G. Espinosa-Montaño ◽  
José L. Uribe-Rubio
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Black Queen Cell Virus ◽  
Scientific Note ◽  
Queen Cell

scholarly journals Black queen cell virus and Nosema ceranae coinfection in Africanized honey bees from southern Brazil

2020 ◽  
Vol 40 (11) ◽  
pp. 892-897
Author(s):  
Domitila B. Chagas ◽  
Francielle Liz Monteiro ◽  
Lariane da S. Barcelos ◽  
Matheus Iuri Frühauf ◽  
Leonardo C. Ribeiro ◽  
...  
Keyword(s):  
Honey Bees ◽  
Nosema Ceranae ◽  
Southern Brazil ◽  
Rt Pcr ◽  
Cdna Synthesis ◽  
Black Queen Cell Virus ◽  
Africanized Honey Bees ◽  
Southern States ◽  
Queen Cell ◽  
Polymerase Chain

ABSTRACT: Bees are fundamental in several aspects, especially in relation to plant biodiversity and pollination. Recently, immense losses are being faced in the number of Brazilian colonies, mainly in southern states of the country, which has a strong beekeeping activity. There are indications that, among the reasons for the losses, pathogens that affect the health of bees may be involved. Among them, the microsporidium Nosema and the black queen cell virus (BQCV) stand out for their prevalence. In this study, 92 colonies of 17 apiaries from southern Brazil were evaluated for infection by Nosema ceranae, Nosema apis and BQCV. Nucleic acid extractions and cDNA synthesis were performed from adult bee samples, followed by Reverse Transcription Polymerase Chain Reaction (RT-PCR) and multiplex PCR. Eight BQCV positive samples were subjected to sequencing. The results showed that N. ceranae and BQCV are circulating in the Southern region of the country, which may be the reason for the loss of colonies. N. apis was not found. N. ceranae was found in 57.6% (53/92) of the colonies and BQCV in 32.6% (30/92). Co-infection was found in 25% (23/92) of the colonies studied, a factor that is suggested to be reducing the hosts’ longevity due to the synergistic action of the pathogens. The samples submitted to sequencing indicated similarity of 96.8 to 100% between them, in addition to strong similarity with sequences from Asia, United States, Germany and Peru. This study reports the circulation of N. ceranae and BQCV in apiaries in southern Brazil, in addition to being the first phylogenetic analysis of the Brazilian BQCV sequence.

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