scholarly journals Streptomyces ferralitis sp. nov., a novel streptomycete isolated from a New-Caledonian ultramafic soil

2004 ◽  
Vol 54 (6) ◽  
pp. 2061-2065 ◽  
Author(s):  
Danielle Saintpierre-Bonaccio ◽  
Hamid Amir ◽  
René Pineau ◽  
Sanaa Lemriss ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
Gene Sequence ◽  
New Caledonia ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Diverse Range ◽  
Ultramafic Soil ◽  
Phyletic Line

The taxonomic position of an actinomycete isolated from an ultramafic soil in New Caledonia was determined using a polyphasic approach. The isolate, which was designated SFOp68T, was shown to have chemical and morphological properties typical of streptomycetes. An almost complete 16S rRNA gene sequence of the isolate was generated and compared with sequences of representative streptomycetes. The 16S rRNA data not only supported the classification of the strain in the genus Streptomyces, but also showed that it formed a distinct phyletic line that was most closely related to one composed of the type strain of Streptomyces rimosus. The two organisms can be readily separated using a diverse range of phenotypic properties. It is proposed that strain SFOp68T (=DSM 41836T=NCIMB 13954T) be classified in the genus Streptomyces as Streptomyces ferralitis sp. nov.

scholarly journals Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China

2007 ◽  
Vol 57 (11) ◽  
pp. 2578-2582 ◽  
Author(s):  
Jun Mao ◽  
Qiyong Tang ◽  
Zhidong Zhang ◽  
Wei Wang ◽  
Dong Wei ◽  
...  
Keyword(s):  
16S Rrna ◽  
Polluted Soil ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
West China ◽  
North West ◽  
Phyletic Line ◽  
Radiation Resistant

The taxonomic position of an actinomycete isolated from radiation-polluted soil collected in Xinjiang Province, north-west China, was determined by using a polyphasic approach. The isolate, designated strain R97T, had chemical and morphological properties characteristic of streptomycetes. An almost-complete 16S rRNA gene sequence of the isolate was generated and compared with corresponding sequences of representative streptomycetes. The 16S rRNA data not only supported the classification of the strain in the genus Streptomyces but also showed that it represented a distinct phyletic line that was most closely, albeit loosely, associated with three other thermotolerant organisms, namely Streptomyces macrosporus NBRC 14748T, Streptomyces megasporus NBRC 14749T and Streptomyces thermolineatus NBRC 14750T. Strain R97T could be distinguished from these organisms based on a range of phenotypic properties. It is proposed that R97T (=CGMCC 4.3519T =DSM 41901T) be classified as the type strain of a novel species in the genus Streptomyces, Streptomyces radiopugnans sp. nov. The organism was shown to be resistant to 60Co gamma radiation at a dose of 15 kGy.

scholarly journals Nocardia neocaledoniensis sp. nov., a novel actinomycete isolated from a New-Caledonian brown hypermagnesian ultramafic soil

2004 ◽  
Vol 54 (2) ◽  
pp. 599-603 ◽  
Author(s):  
Danielle Saintpierre-Bonaccio ◽  
Luis A. Maldonado ◽  
Hamid Amir ◽  
René Pineau ◽  
Michael Goodfellow
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Sequence Data ◽  
Morphological Properties ◽  
Nocardia Asteroides ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Ultramafic Soil

The taxonomic position of an actinomycete isolated from a hypermagnesian ultramafic soil was examined using a polyphasic approach. The strain, designated SBHR OA6T, was shown to have chemical and morphological properties typical of members of the genus Nocardia. The organism was most closely associated with Nocardia asteroides using 16S rRNA gene sequence data. It showed a distinctive set of phenotypic properties that distinguished it from representatives of all species with validly published names classified in the genus Nocardia. The combined genotypic and phenotypic data show that strain SBHR OA6T (=DSM 44717T=NCIMB 13955T) merits description as the type strain of a novel Nocardia species, Nocardia neocaledoniensis sp. nov.

scholarly journals Amycolatopsis plumensis sp. nov., a novel bioactive actinomycete isolated from a New-Caledonian brown hypermagnesian ultramafic soil

2005 ◽  
Vol 55 (5) ◽  
pp. 2057-2061 ◽  
Author(s):  
Danielle Saintpierre-Bonaccio ◽  
Hamid Amir ◽  
René Pineau ◽  
G. Y. Annie Tan ◽  
Michael Goodfellow
Keyword(s):  
Gene Sequence ◽  
Sequence Data ◽  
Novel Species ◽  
Morphological Properties ◽  
Rrna Gene ◽  
The Novel ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Ultramafic Soil ◽  
Type Strains

The taxonomic position of an actinomycete isolated from a brown hypermagnesian ultramafic soil was examined using a polyphasic approach. The organism, which was designated SBHS Strp1T, was found to have chemical and morphological properties typical of Amycolatopsis strains. It was most closely associated with Amycolatopsis kentuckyensis, Amycolatopsis lexingtonensis, Amycolatopsis rifamycinica, Amycolatopsis pretoriensis and Amycolatopsis tolypomycina on the basis of 16S rRNA gene sequence data, and showed a unique pattern of phenotypic properties that distinguished it from the type strains of these taxa. The combined genotypic and phenotypic data show that the organism merits description as a novel species of Amycolatopsis. The name proposed for the novel species is Amycolatopsis plumensis sp. nov.; the type strain is SBHS Strp1T (=DSM 44776T=NRRL B-24324T).

scholarly journals Streptomyces cocklensis sp. nov., a dioxamycin-producing actinomycete

2012 ◽  
Vol 62 (2) ◽  
pp. 279-283 ◽  
Author(s):  
Byung-Yong Kim ◽  
Tiago Domingues Zucchi ◽  
Hans-Peter Fiedler ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Gene Sequence Analysis ◽  
Phyletic Line

The taxonomic position of a streptomycete isolated from soil collected from Cockle Park Experimental Farm, Northumberland, UK, was determined by using a polyphasic approach. The organism had chemical and morphological features consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis supported classification of the strain in the genus Streptomyces and showed that it formed a distinct phyletic line loosely associated with members of the Streptomyces yeochonensis clade. It was related most closely to Streptomyces paucisporeus 1413T (98.6 %16S rRNA gene sequence similarity), but could be distinguished from the latter based on the low level of DNA–DNA relatedness (40 %). It was readily distinguished from the type strains of all species assigned to the S. yeochonensis clade based on a combination of phenotypic properties. Strain BK168T ( = KACC 20908T = NCIMB 14704T) should therefore be classified as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces cocklensis sp. nov. is proposed. The organism produces the antibiotic dioxamycin.

scholarly journals Lechevalieria fradiae sp. nov., a novel actinomycete isolated from soil in China

2007 ◽  
Vol 57 (4) ◽  
pp. 832-836 ◽  
Author(s):  
Jianli Zhang ◽  
Qiong Xie ◽  
Zhiheng Liu ◽  
Michael Goodfellow
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Morphological Properties ◽  
Rrna Gene ◽  
New Taxon ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Soil Isolate ◽  
Evolutionary Radiation ◽  
Phyletic Line

The taxonomic position of a soil isolate, strain Z6T, was established using a polyphasic approach. The organism showed a range of chemical and morphological properties consistent with its classification in the genus Lechevalieria. An almost complete 16S rRNA gene sequence determined for the strain was aligned with corresponding sequences of representatives of the genus Lechevalieria and related taxa using three tree-making algorithms. The organism formed a distinct phyletic line within the evolutionary radiation occupied by the genus Lechevalieria and was more closely related to the type strain of Lechevalieria aerocolonigenes than to that of Lechevalieria flava. Strain Z6T could be distinguished from both these strains using DNA–DNA relatedness data and by a combination of phenotypic properties. The combined genotypic and phenotypic data show that strain Z6T should be assigned to the genus Lechevalieria as a representative of a novel species. The name proposed for this new taxon is Lechevalieria fradiae sp. nov. The type strain is Z6T (=CGMCC 4.3506T=JCM 14205T).

scholarly journals Streptomyces glauciniger sp. nov., a novel mesophilic streptomycete isolated from soil in south China

2004 ◽  
Vol 54 (6) ◽  
pp. 2085-2089 ◽  
Author(s):  
Ying Huang ◽  
Wei Li ◽  
Liming Wang ◽  
Benjamin Lanoot ◽  
Marc Vancanneyt ◽  
...  
Keyword(s):  
16S Rrna ◽  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Sharp Separation ◽  
Rdna Its ◽  
Phyletic Line

A polyphasic study was undertaken to establish the taxonomic status of a soil isolate. The organism, strain FXJ14T, was found to have chemical and morphological properties characteristic of streptomycetes. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence of the strain and on the 120 nt variable γ-region of the 16S rRNA molecule showed that it formed a distinct phyletic line within the range of variation encompassed by the genus Streptomyces. The sharp separation of the organism from representatives of the genus Streptomyces was strengthened by the fact that its BOX-PCR and RFLP of 16S rDNA-ITS fingerprints differed from those of over 450 recognized Streptomyces species. The isolate also had a profile of phenotypic properties that readily distinguished it from the genotypically close type strains. It is evident from the combination of genotypic and phenotypic data that strain FXJ14T (=AS 4.1858T=JCM 12278T=LMG 22082T) should be classified as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces glauciniger sp. nov. is proposed.

scholarly journals Nocardia xishanensis sp. nov., a novel actinomycete isolated from soil

2004 ◽  
Vol 54 (6) ◽  
pp. 2301-2305 ◽  
Author(s):  
Jianli Zhang ◽  
Zhiheng Liu ◽  
Michael Goodfellow
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Morphological Properties ◽  
Rrna Gene ◽  
New Taxon ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Soil Isolate ◽  
Evolutionary Radiation ◽  
Phyletic Line

The taxonomic position of a soil isolate, strain 276T, was established using a polyphasic approach. The organism showed a range of chemical and morphological properties consistent with its classification in the genus Nocardia. An almost complete 16S rRNA gene sequence determined for the strain was aligned with corresponding sequences of representatives of the genus Nocardia and related taxa using three tree-making algorithms. The organism formed a distinct phyletic line within the evolutionary radiation occupied by the genus Nocardia and was most closely related to the type strain of Nocardia abscessus. However, the two strains shared a low DNA–DNA relatedness value and were readily distinguished using a combination of phenotypic properties. The combined genotypic and phenotypic data show that strain 276T should be assigned to the genus Nocardia as a novel species. The name proposed for this new taxon is Nocardia xishanensis sp. nov. The type strain is 276T (=CGMCC 4.1860T=JCM 12160T).

scholarly journals Pedobacter boryungensis sp. nov., isolated from soil

2012 ◽  
Vol 62 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Yong-Taek Jung ◽  
Soo-Young Lee ◽  
Won-Chan Choi ◽  
Tae-Kwang Oh ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Type Strains

A Gram-negative, non-sporulating, non-flagellated rod, designated BR-9T, was isolated from soil collected on the Korean peninsula. Strain BR-9T grew optimally at pH 6.0–7.0, at 30 °C and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BR-9T belonged to the genus Pedobacter and clustered with Pedobacter insulae DS-139T and Pedobacter koreensis WPCB189T. Strain BR-9T exhibited 98.2 and 97.5 % 16S rRNA gene sequence similarity with P. insulae DS-139T and P. koreensis WPCB189T, respectively, and <96.7 % sequence similarity with the type strains of other species in the genus Pedobacter. Strain BR-9T contained MK-7 as the predominant menaquinone and iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) as the major fatty acids. The DNA G+C content of strain BR-9T was 38.5 mol%. DNA–DNA relatedness between strain BR-9T and P. insulae DS-139T and P. koreensis KCTC 12536T was 3.4–4.2 %, which indicated that the isolate was genetically distinct from these type strains. Strain BR-9T was also distinguishable by differences in phenotypic properties. On the basis of the data presented, strain BR-9T is considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter boryungensis sp. nov. is proposed. The type strain is BR-9T ( = KCTC 23344T  = CCUG 60024T).

scholarly journals Pseudomonas taeanensis sp. nov., isolated from a crude oil-contaminated seashore

2010 ◽  
Vol 60 (12) ◽  
pp. 2719-2723 ◽  
Author(s):  
Dong-Heon Lee ◽  
Sung-Ran Moon ◽  
Young-Hyun Park ◽  
Jung-Ho Kim ◽  
Hoon Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Crude Oil ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Low Levels

A novel Gram-negative, aerobic, motile, short rod-shaped bacterium, designated MS-3T, was isolated from a crude oil-contaminated seashore in Taean, Korea. Strain MS-3T grew at 4–30 °C, at pH 6.0–9.5 and with 0–5 % NaCl and was oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MS-3T was most similar to Pseudomonas marincola KMM 3042T (97.9 % 16S rRNA gene sequence similarity), P. cuatrocienegasensis 1NT (97.8 %), P. borbori R-20821T (97.3 %) and P. lundensis ATCC 49968T (97.1 %). Relatively low levels of DNA–DNA relatedness were found between strain MS-3T and P. cuatrocienegasensis LMG 24676T (57.2 %), P. borbori LMG 23199T (39.7 %), P. marincola KMM 3042T (32.2 %) and P. lundensis KACC 10832T (32.1 %), which support the classification of strain MS-3T within a novel species of the genus Pseudomonas. The G+C content of the genomic DNA of strain MS-3T was 57.6 mol% and the major isoprenoid quinone was Q-9. Strain MS-3T contained summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c; 38.0 %), C16 : 0 (24.4 %), C18 : 1 ω7c (12.8 %), C12 : 0 (9.6 %) and C10 : 0 3-OH (4.9 %) as the major cellular fatty acids. On the basis of the phenotypic, genotypic and phylogenetic data, strain MS-3T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas taeanensis sp. nov. is proposed. The type strain is MS-3T (=KCTC 22612T =KACC 14032T =JCM 16046T =NBRL 105641T).

scholarly journals Sphingobacterium shayense sp. nov., isolated from forest soil

2010 ◽  
Vol 60 (10) ◽  
pp. 2377-2381 ◽  
Author(s):  
Xiang He ◽  
Ting Xiao ◽  
Haiju Kuang ◽  
Xiaojun Lan ◽  
Maripat Tudahong ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Populus Euphratica ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties

A Gram-staining-negative, yellow-coloured, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated HS39T, isolated from a soil sample collected from a natural Populus euphratica forest in Xinjiang, China, was characterized using a polyphasic approach. The isolate grew optimally at 30–37 °C, at pH 6.5–8.0 and with 0–3 % NaCl. Analysis of the 16S rRNA gene sequence of strain HS39T revealed that it is a member of the genus Sphingobacterium. Sphingobacterium mizutaii ATCC 33299T was the nearest relative (94.0 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 40.2 mol%. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic properties and phylogenetic inference, strain HS39T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium shayense sp. nov. is proposed. The type strain is HS39T (=CCTCC AB 209006T =NRRL B-59203T).

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