scholarly journals Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China

2007 ◽  
Vol 57 (11) ◽  
pp. 2578-2582 ◽  
Author(s):  
Jun Mao ◽  
Qiyong Tang ◽  
Zhidong Zhang ◽  
Wei Wang ◽  
Dong Wei ◽  
...  
Keyword(s):  
16S Rrna ◽  
Polluted Soil ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
West China ◽  
North West ◽  
Phyletic Line ◽  
Radiation Resistant

The taxonomic position of an actinomycete isolated from radiation-polluted soil collected in Xinjiang Province, north-west China, was determined by using a polyphasic approach. The isolate, designated strain R97T, had chemical and morphological properties characteristic of streptomycetes. An almost-complete 16S rRNA gene sequence of the isolate was generated and compared with corresponding sequences of representative streptomycetes. The 16S rRNA data not only supported the classification of the strain in the genus Streptomyces but also showed that it represented a distinct phyletic line that was most closely, albeit loosely, associated with three other thermotolerant organisms, namely Streptomyces macrosporus NBRC 14748T, Streptomyces megasporus NBRC 14749T and Streptomyces thermolineatus NBRC 14750T. Strain R97T could be distinguished from these organisms based on a range of phenotypic properties. It is proposed that R97T (=CGMCC 4.3519T =DSM 41901T) be classified as the type strain of a novel species in the genus Streptomyces, Streptomyces radiopugnans sp. nov. The organism was shown to be resistant to 60Co gamma radiation at a dose of 15 kGy.

scholarly journals Streptomyces ferralitis sp. nov., a novel streptomycete isolated from a New-Caledonian ultramafic soil

2004 ◽  
Vol 54 (6) ◽  
pp. 2061-2065 ◽  
Author(s):  
Danielle Saintpierre-Bonaccio ◽  
Hamid Amir ◽  
René Pineau ◽  
Sanaa Lemriss ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
Gene Sequence ◽  
New Caledonia ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Diverse Range ◽  
Ultramafic Soil ◽  
Phyletic Line

The taxonomic position of an actinomycete isolated from an ultramafic soil in New Caledonia was determined using a polyphasic approach. The isolate, which was designated SFOp68T, was shown to have chemical and morphological properties typical of streptomycetes. An almost complete 16S rRNA gene sequence of the isolate was generated and compared with sequences of representative streptomycetes. The 16S rRNA data not only supported the classification of the strain in the genus Streptomyces, but also showed that it formed a distinct phyletic line that was most closely related to one composed of the type strain of Streptomyces rimosus. The two organisms can be readily separated using a diverse range of phenotypic properties. It is proposed that strain SFOp68T (=DSM 41836T=NCIMB 13954T) be classified in the genus Streptomyces as Streptomyces ferralitis sp. nov.

scholarly journals Streptomyces glauciniger sp. nov., a novel mesophilic streptomycete isolated from soil in south China

2004 ◽  
Vol 54 (6) ◽  
pp. 2085-2089 ◽  
Author(s):  
Ying Huang ◽  
Wei Li ◽  
Liming Wang ◽  
Benjamin Lanoot ◽  
Marc Vancanneyt ◽  
...  
Keyword(s):  
16S Rrna ◽  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Sharp Separation ◽  
Rdna Its ◽  
Phyletic Line

A polyphasic study was undertaken to establish the taxonomic status of a soil isolate. The organism, strain FXJ14T, was found to have chemical and morphological properties characteristic of streptomycetes. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence of the strain and on the 120 nt variable γ-region of the 16S rRNA molecule showed that it formed a distinct phyletic line within the range of variation encompassed by the genus Streptomyces. The sharp separation of the organism from representatives of the genus Streptomyces was strengthened by the fact that its BOX-PCR and RFLP of 16S rDNA-ITS fingerprints differed from those of over 450 recognized Streptomyces species. The isolate also had a profile of phenotypic properties that readily distinguished it from the genotypically close type strains. It is evident from the combination of genotypic and phenotypic data that strain FXJ14T (=AS 4.1858T=JCM 12278T=LMG 22082T) should be classified as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces glauciniger sp. nov. is proposed.

scholarly journals Streptomyces brevispora sp. nov. and Streptomyces laculatispora sp. nov., actinomycetes isolated from soil

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 478-483 ◽  
Author(s):  
Tiago Domingues Zucchi ◽  
Byung-yong Kim ◽  
Jenileima Devi Kshetrimayum ◽  
Hang-Yeon Weon ◽  
Soon-Wo Kwon ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Meadow Soil ◽  
Phenotypic Properties ◽  
Dna Relatedness ◽  
Respective Type ◽  
Type Strains

The taxonomic positions of two actinomycetes isolated from a hay meadow soil sample were determined using a polyphasic approach. The isolates had chemical and morphological properties typical of streptomycetes and formed a distinct 16S rRNA gene subclade together with the type strain Streptomyces drozdowiczii NRRL B-24297T. DNA–DNA relatedness studies showed that the three strains belonged to different genomic species. The organisms were also distinguished using a combination of phenotypic properties. On the basis of these data it is proposed that the isolates be assigned to the genus Streptomyces as Streptomyces brevispora sp. nov. and Streptomyces laculatispora sp. nov., with BK160T ( = KACC 21093T  = NCIMB 14702T) and BK166T ( = KACC 20907T  = NCIMB 14703T) as the respective type strains.

scholarly journals Amycolatopsis salitolerans sp. nov., a filamentous actinomycete isolated from a hypersaline habitat

2012 ◽  
Vol 62 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Tong-Wei Guan ◽  
Zhan-Feng Xia ◽  
Shu-Kun Tang ◽  
Nan Wu ◽  
Zheng-Jun Chen ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
West China ◽  
North West ◽  
Actinomycete Strain

A novel actinomycete strain, designated TRM F103T, was isolated from a hypersaline habitat of the Tarim basin in Xinjiang province, north-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Amycolatopsis and was most closely related to Amycolatopsis halophila YIM 93223T (99.3 % 16S rRNA gene sequence similarity). However, DNA–DNA relatedness between these two strains, based on triplicate experiments, was only 31.6 %. The isolate contained meso-diaminopimelic acid and ribose, glucose and galactose as the major whole-cell sugars. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C16 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and glucosamine-containing phospholipids. The G+C content of the genomic DNA was 66.4 mol%. The phenotypic data clearly distinguished the isolate from its closest relatives. The combined phylogenetic, chemotaxonomic and phenotypic data indicate that the isolate represents a novel species of the genus Amycolatopsis. The proposed name is Amycolatopsis salitolerans sp. nov., with TRM F103T ( = JCM 15899T = CCTCC AB 208326T) as the type strain.

scholarly journals Zhihengliuella halotolerans gen. nov., sp. nov., a novel member of the family Micrococcaceae

2007 ◽  
Vol 57 (5) ◽  
pp. 1018-1023 ◽  
Author(s):  
Yu-Qin Zhang ◽  
Peter Schumann ◽  
Li-Yan Yu ◽  
Hong-Yu Liu ◽  
Yue-Qin Zhang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molar Ratio ◽  
Rrna Gene ◽  
West China ◽  
Qinghai Province ◽  
North West ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Unknown Phospholipid

The actinobacterial strain YIM 70185T was isolated from a saline soil sample collected from Qinghai province, north-west China, and subjected to a taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences revealed 93.5–96.4 % similarity to members of related genera in the family Micrococcaceae. In the phylogenetic dendrogram based on 16S rRNA gene sequence analysis, strain YIM 70185T formed a separate clade next to the genera Micrococcus and Citricoccus within the family Micrococcaceae. The peptidoglycan type was A4α, l-lys–l-ala–l-Glu. Cell-wall sugars contained glucose and tyvelose. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and an unknown glycolipid. The menaquinones were MK-9, MK-10 and MK-8 (molar ratio 5 : 2 : 1). The major fatty acids were ai-C15 : 0 and i-C15 : 0 and the DNA G+C content was 66.5 mol%. These chemotaxonomic profiles supported the assignment of strain YIM 70185T to a novel genus within the family Micrococcaceae. The name Zhihengliuella halotolerans gen. nov., sp. nov. is proposed. The type strain of Zhihengliuella halotolerans is YIM 70185T (=DSM 17364T=KCTC 19085T).

scholarly journals Lechevalieria xinjiangensis sp. nov., a novel actinomycete isolated from radiation-polluted soil in China

2007 ◽  
Vol 57 (12) ◽  
pp. 2819-2822 ◽  
Author(s):  
Wei Wang ◽  
Zhidong Zhang ◽  
Qiyong Tang ◽  
Jun Mao ◽  
Dong Wei ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Polluted Soil ◽  
Western China ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Data ◽  
Phyletic Line ◽  
North Western

A novel actinomycete was isolated from radiation-polluted soil collected from Xinjiang in north-western China. The isolate, strain R24T, was found to have chemical and morphological properties associated with members of the genus Lechevalieria. An almost-complete 16S rRNA gene sequence of the isolate was aligned with corresponding sequences from representatives of the genus Lechevalieria and related taxa, using three tree-making algorithms, and was shown to form a distinct phyletic line in the Lechevalieria phylogenetic tree. DNA–DNA relatedness and phenotypic data served to distinguish strain R24T from members of the three Lechevalieria species with validly published names. The genotypic and phenotypic data show that the isolate should be classified as a novel species within the genus Lechevalieria. The name proposed for this taxon is Lechevalieria xinjiangensis sp. nov. The type strain is R24T (=CGMCC 4.3525T=DSM 45081T).

Actinophytocola xinjiangensis sp. nov., isolated from virgin forest soil

2011 ◽  
Vol 61 (12) ◽  
pp. 2928-2932 ◽  
Author(s):  
Xiaoxuan Guo ◽  
Danheng Qiu ◽  
Jisheng Ruan ◽  
Ying Huang
Keyword(s):  
Forest Soil ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
West China ◽  
North West ◽  
Virgin Forest ◽  
Phyletic Line ◽  
Isoprenoid Quinones

An aerobic, non-motile actinobacterium, strain QAIII60T, was isolated from virgin forest soil of Kanas Nature Reserve, Xinjiang, north-west China. The isolate produced a very scant aerial mycelium that fragmented into cylindrical spores and a non-fragmented substrate mycelium with occasional septa. Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose, galactose, glucose, ribose and rhamnose (trace). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. The major cellular fatty acids were iso-C16 : 0, iso-C14 : 0, iso-C16 : 1 H and C17 : 1ω6c. The isoprenoid quinones consisted of MK-9(H4) and MK-10(H2). The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QAIII60T formed a distinct phyletic line that was most closely, albeit loosely, associated with the genus Actinophytocola. A number of physiological characteristics differentiated the isolate from members of the genus Actinophytocola. On the basis of these data, we propose that strain QAIII60T ( = CGMCC 4.4663T  = NBRC 106673T) be assigned as the type strain of a novel species, Actinophytocola xinjiangensis sp. nov.

scholarly journals Nocardiopsis flavescens sp. nov., an actinomycete isolated from marine sediment

2011 ◽  
Vol 61 (11) ◽  
pp. 2640-2645 ◽  
Author(s):  
Caiyuan Fang ◽  
Jianli Zhang ◽  
Huancheng Pang ◽  
Yuyi Li ◽  
Yuhua Xin ◽  
...  
Keyword(s):  
Marine Sediment ◽  
Type Strain ◽  
Diaminopimelic Acid ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Phenotypic Properties ◽  
Evolutionary Radiation ◽  
Phyletic Line ◽  
Bacterium Strain

A Gram-positive, aerobic bacterium, strain SA6T, was isolated from marine sediment taken at a depth of 20 cm on the seashore of Lianyungang, Jiangsu Province, China. Strain SA6T contained meso-diaminopimelic acid, no diagnostic sugars, type PIII phospholipids, and MK-10(H2) and MK-10(H4) as the predominant menaquinones. The organism showed a range of chemical and morphological properties consistent with its classification in the genus Nocardiopsis. The almost-complete 16S rRNA gene sequence of strain SA6T was aligned with corresponding sequences of representatives of the genus Nocardiopsis and related taxa by using two tree-making algorithms. Strain SA6T formed a distinct phyletic line within the evolutionary radiation occupied by the genus Nocardiopsis and was related most closely to the type strain of Nocardiopsis lucentensis. Strain SA6T could be distinguished from its nearest phylogenetic relatives in the genus Nocardiopsis based on DNA–DNA relatedness data and a combination of phenotypic properties. Strain SA6T should therefore be assigned to the genus Nocardiopsis as a representative of a novel species, for which the name Nocardiopsis flavescens sp. nov. is proposed. The type strain is SA6T ( = CGMCC 4.5723T  = JCM 17424T).

scholarly journals Williamsia faeni sp. nov., an actinomycete isolated from a hay meadow

2010 ◽  
Vol 60 (11) ◽  
pp. 2548-2551 ◽  
Author(s):  
Amanda L. Jones ◽  
Gail D. Payne ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phyletic Line ◽  
Type Strains ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

The taxonomic status of an actinomycete isolated from soil collected from a hay meadow was determined using a polyphasic approach. The strain, designated N1350T, had morphological and chemotaxonomic properties consistent with its classification in the genus Williamsia and formed a distinct phyletic line within the clade comprising the type strains of species of the genus Williamsia in the 16S rRNA gene tree. Strain N1350T shared highest 16S rRNA gene sequence similarities with Williamsia marianensis MT8T (98.1 %) and Williamsia muralis MA140-96T (98.3 %). However, strain N1350T was readily distinguished from the type strains of Williamsia species using a combination of phenotypic properties. On the basis of these data, strain N1350T is considered to represent a novel species of the genus Williamsia. The name proposed for this taxon is Williamsia faeni sp. nov., with the type strain N1350T (=DSM 45372T =NCIMB 14575T =NRRL B-24794T).

scholarly journals Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov., nom. rev., isolated from soil

2010 ◽  
Vol 60 (8) ◽  
pp. 1813-1823 ◽  
Author(s):  
Byung-Yong Kim ◽  
James E. M. Stach ◽  
Hang-Yeon Weon ◽  
Soon-Wo Kwon ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Morphological Properties ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Gene Sequence Analysis ◽  
Type Strains

Forty strains isolated from soil taken from a hay meadow were assigned to the genus Dactylosporangium on the basis of colonial properties. 16S rRNA gene sequence analysis showed that the isolates formed a group that was most closely related to the type strain of Dactylosporangium aurantiacum, but well separated from other Dactylosporangium type strains and from ‘Dactylosporangium salmoneum’ NRRL B-16294. Twelve of 13 representative isolates had identical 16S rRNA gene sequences and formed a subclade that was distinct from corresponding phyletic lines composed of the remaining isolate, strain BK63T, the ‘D. salmoneum’ strain and the type strains of recognized Dactylosporangium species. DNA–DNA relatedness data indicated that representatives of the multi-membered 16S rRNA gene subclade, isolate BK63T and the ‘D. salmoneum’ subclade formed distinct genomic species; all of these organisms had chemotaxonomic and morphological properties consistent with their classification in the genus Dactylosporangium. They were also distinguished from one another and from the type strains of recognized Dactylosporangium species based on a range of phenotypic properties. Combined genotypic and phenotypic data showed that isolate BK63T, isolates BK51T, BK53 and BK69, and strain NRRL B-16294T should be classified in the genus Dactylosporangium as representing novel species. The names proposed for these species are Dactylosporangium luridum sp. nov. (type strain BK63T  = DSM 45324T  = KACC 20933T  = NRRL B-24775T), Dactylosporangium luteum sp. nov. (type strain BK51T  = DSM 45323T  = KACC 20899T  = NRRL B-24774T) and Dactylosporangium salmoneum sp. nov., nom. rev. (type strain NRRL B-16294T  = ATCC 31222T  = DSM 43910T  = JCM 3272T  = NBRC 14103T).

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