Inability to accumulate Ni in a genus of hyperaccumulators: the paradox of Odontarrhena sibirica (Brassicaceae)

Main conclusion Odontarrhena is a highly diverse genus of Ni-hyperaccumulators. Here, we demonstrate substantial inability to accumulate Ni in the facultative serpentinophyte O. sibirica, which seems a unique case among the numerous species of the genus that grow on ultramafic soils. Abstract Odontarrhena is the most diverse genus of Ni-accumulating plants in W Eurasia, with most taxa growing obligatorily or facultatively on ultramafic soils. A notable exception may be O. sibirica, a facultative serpentinophyte from the E Mediterranean and W Asia in which accumulation ability is still enigmatic. We addressed this issue using observational and experimental methods. Atomic Absorption Analysis of 33 herbarium specimens and plant and soil samples from seven ultramafic and non-ultramafic sites in Greece revealed shoot Ni values always much lower than 1000 µg g−1, non-significant differences between plants from the two soil types and no relationship with soil pH. Only two Turkish specimens from waste mines had shoot Ni concentration > 1000 µg g−1. The reasons for this deviating result remain obscure, but may be associated with inherent peculiarities of the local populations. When cultivated together with congeneric Ni-accumulating species on the same natural ultramafic soil, only O. sibirica was unable to accumulate the metal. Although plant growth was stimulated in hydroponics at relatively low NiSO4 levels (50–150 µM), as typical for hyperaccumulators, Ni-accumulation occurred only at higher concentrations which had a toxic effect. This peculiar combination of Ni-response traits could be the result of a partial evolutionary loss of ability with respect to all other Ni-accumulating congeneric species. For this, O. sibirica could represent a unique model system for further studies on the evolutionary dynamics, physiological mechanisms and genetic control of metal accumulation and homeostasis.

Contaminated Land by Wildfire Effect on Ultramafic Soil and Associated Human Health and Ecological Risk

The purpose of this study is the evaluation of fire effect on contaminated land and the assessment of the associated risk of human health and terrestrial ecological receptors. Ash and soil samples were gathered from burned and unburned areas (central Evia, Greece) which are adjacent with a Natura 2000 area. The geochemical dataset includes 20 sampling sites and 35 elements. The wildfire severity was investigated by applying a macroscopic approach and field observations. Statistical and spatial analysis were applied for delineating the distribution of elements in ash and soil. Elemental balance approach was performed for estimating net gain (+) or loss (−) to the ash. Element contents in sampling sites were compared to screening values proposed by the literature. Hundreds of hectares of burned land including wildland areas in central Evia are contaminated with (contents in mg Kg−1), Co (up to 43.5), Cr (up to 244), Mn (up to 1158), Ni (up to 463) associated with geogenic sources such as serpentinite peridotites and Ni-laterite deposits. Aluminum, As, Cd, Co, Cr, Fe, Mn, Ni, Pb, V and Zn contents recorded in the sampling sites are posing a potential risk to human health and ecological receptors.

Ecophysiology of nickel hyperaccumulating plants from South Africa – from ultramafic soil and mycorrhiza to plants and insects

Ecophysiological model “ultramafic soil – mycorrhiza – hyperaccumulating plants – specialised insects and other organisms” is presented for South African nickel hyperaccumulating plants.

New Caledonian ultramafic conditions structure the features of Curtobacterium citreum strains that play a role in plant adaptation

The present study focused on the characterization of 10 Curtobacterium citreum strains isolated from the rhizosphere of pioneer plants growing on ultramafic soils from New Caledonia. Taxonomic status was investigated using a polyphasic approach. Three strains (BE, BB, and AM) were selected in terms of multiple-metal resistance and plant-growth-promoting traits. They were tested on sorghum growing on ultramafic soil and compared with the reference strain C. citreum DSM20528T. To better understand the bacterial mechanisms involved, biosorption, bioaccumulation, and biofilm formation were investigated for the representative strain of the ultramafic cluster (strain BE) versus C. citreum DSM20528T. The polyphasic approach confirmed that all native isolates belong to the same cluster and are C. citreum. The inoculation of sorghum with strains BE and BB significantly reduced Ni content in shoots compared with inoculation with C. citreum DSM20528T and control values. This result was related to the higher Ni tolerance of the ultramafic strains compared with C. citreum DSM20528T. Ni biosorption and bioaccumulation showed that BE exhibited a lower Ni content, which is explained by the ability of this strain to produce exopolysaccharides involved in Ni chelation. We suggested that ultramafic C. citreum strains are more adapted to this substrate than is C. citreum DSM20528T, and their features allow them to enhance plant metal tolerance.