Texcoconibacillus texcoconensis gen. nov., sp. nov., alkalophilic and halotolerant bacteria isolated from soil of the former lake Texcoco (Mexico)
A novel Gram-positive, rod-shaped, spore-forming bacterium, designated 13CCT was isolated from soil of the former lake Texcoco. The strain was aerobic, catalase-positive and oxidase-negative. It grew at salinities of 0–26 % (w/v) NaCl with an optimum at 9–16 % (w/v) NaCl. The cells contain peptidoglycan type A1γ, A1γ′ with glycine instead of l-alanine and three variations of peptidoglycan type A4γ. The only quinone detected was MK-7. The major fatty acid was anteiso-C15 : 0. The polar lipids fraction consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three different phospholipids. The DNA G+C content was 37.5 mol%. Maximum-likelihood phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 13CCT was closely related to members of the genus Bacillus and shared 92.35 % similarity with Bacillus agaradhaerens , 92.28 % with Bacillus neizhouensis and 92.21 % with Bacillus locisalis . It is proposed based on the phenotypic, genotypic and phylogenetic analyses that the novel isolate should be classified as a representative of a new genus and novel species, for which the name Texcoconibacillus texcoconensis gen. nov., sp. nov. is proposed. The type strain of Texcoconibacillus texcoconensis is 13CCT ( = JCM 17654T = DSM 24696T).