scholarly journals Desulfoprunum benzoelyticum gen. nov., sp. nov., a Gram-stain-negative, benzoate-degrading, sulfate-reducing bacterium isolated from a wastewater treatment plant

2015 ◽  
Vol 65 (Pt_1) ◽  
pp. 77-84 ◽  
Author(s):  
Madan Junghare ◽  
Bernhard Schink
Keyword(s):  
Fatty Acids ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Type Species ◽  
Treatment Plant ◽  
Strictly Anaerobic ◽  
Gram Stain ◽  
Content Type ◽  
Sulfate Reducing ◽  
Link Type

A strictly anaerobic, mesophilic, sulfate-reducing bacterium, strain KoBa311T, isolated from the wastewater treatment plant at Konstanz, Germany, was characterized phenotypically and phylogenetically. Cells were Gram-stain-negative, non-motile, oval to short rods, 3–5 µm long and 0.8–1.0 µm wide with rounded ends, dividing by binary fission and occurring singly or in pairs. The strain grew optimally in freshwater medium and the optimum temperature was 30 °C. Strain KoBa311T showed optimum growth at pH 7.3−7.6. Organic electron donors were oxidized completely to carbon dioxide concomitant with sulfate reduction to sulfide. At excess substrate supply, substrates were oxidized incompletely and acetate (mainly) and/or propionate accumulated. The strain utilized short-chain fatty acids, alcohols (except methanol) and benzoate. Sulfate and DMSO were used as terminal electron acceptors for growth. The genomic DNA G+C content was 52.3 mol% and the respiratory quinone was menaquinone MK-5 (V-H2). The major fatty acids were C16 : 0, C16 : 1ω7c/ω6c and C18 : 1ω7c. Phylogenetic analysis based on 16S rRNA gene sequences placed strain KoBa311T within the family Desulfobulbaceae in the class Deltaproteobacteria . Its closest related bacterial species on the basis of the distance matrix were Desulfobacterium catecholicum DSM 3882T (93.0 % similarity), Desulfocapsa thiozymogenes (93.1 %), Desulforhopalus singaporensis (92.9 %), Desulfopila aestuarii (92.4 %), Desulfopila inferna JS_SRB250LacT (92.3 %) and Desulfofustis glycolicus (92.3 %). On the basis of phylogenetic, physiological and chemotaxonomic characteristics, strain KoBa311T was distinct from any related type species. Therefore, strain KoBa311T is considered to represent a novel species of a new genus, for which the name Desulfoprunum benzoelyticum gen. nov., sp. nov. is proposed. The type strain of Desulfoprunum benzoelyticum is KoBa311T ( = DSM 28570T = KCTC 15441T).

Nocardioides daeguensis sp. nov., a nitrate-reducing bacterium isolated from activated sludge of an industrial wastewater treatment plant

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3727-3732 ◽  
Author(s):  
Yingshun Cui ◽  
Sung-Geun Woo ◽  
Jangho Lee ◽  
Sahastranshu Sinha ◽  
Myung-Suk Kang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Industrial Wastewater ◽  
Type Species ◽  
Treatment Plant ◽  
Diaminopimelic Acid ◽  
Industrial Wastewater Treatment ◽  
Content Type ◽  
Link Type

A Gram-reaction-positive, rod-shaped, non-spore-forming bacterium (strain 2C1-5T) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6 % similarity), N. kongjuensis (98.5 %), N. caeni (98.4 %), N. simplex (98.3 %), N. aromaticivorans (98.1 %) and N. ginsengisoli (97.5 %); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3 %. Strain 2C1-5T was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C16 : 0, C16 : 0 and C17 : 1ω6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol%. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5T to the genus Nocardioides . The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5T from existing species with validly published names. Therefore, strain 2C1-5T represents a novel species of the genus Nocardioides , for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5T ( = JCM 17460T = KCTC 19799T).

Brevibacterium ammoniilyticum sp. nov., an ammonia-degrading bacterium isolated from sludge of a wastewater treatment plant

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 1111-1118 ◽  
Author(s):  
Jinsoo Kim ◽  
Sathiyaraj Srinivasan ◽  
Taek You ◽  
John J. Bang ◽  
Sujeong Park ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Type Species ◽  
Treatment Plant ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Link Type ◽  
Degrading Bacterium

A Gram-stain-positive, non-motile, chemo-organotrophic, mesophilic, aerobic bacterium, designated A1T, was isolated from sludge of a wastewater treatment plant. Strain A1T showed good ability to degrade ammonia and grew well on media amended with methanol and ammonia. Strain A1T grew with 0–11 % (w/v) NaCl, at 20–42 °C, but not <15 or >45 °C and at pH 6–10 (optimum pH 8.0–9.0). The isolate was catalase-positive and oxidase-negative. The DNA G+C content was 70.7 mol%. A comparative analysis of 16S rRNA gene sequences revealed that strain A1T formed a distinct phyletic lineage in the genus Brevibacterium and showed high sequence similarity with Brevibacterium casei NCDO 2048T (96.9 %), Brevibacterium celere KMM 3637T (96.9 %) and Brevibacterium sanguinis CF63T (96.4 %). DNA–DNA hybridization revealed <43 % DNA–DNA relatedness between the isolate and its closest phylogenetic relatives. The affiliation of strain A1T with the genus Brevibacterium was supported by the chemotaxonomic data: predominant quinone menaquinone MK-7(H2); polar lipid profile containing diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid; characteristic cell-wall diamino acid meso-diaminopimelic acid; whole-cell sugars galactose, xylose and ribose; absence of mycolic acids; and major fatty acids iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The results of physiological and biochemical tests allowed phenotypic differentiation of strain A1T from members of the genus Brevibacterium . On the basis of the results in this study, a novel species, Brevibacterium ammoniilyticum sp. nov., is proposed. The type strain is A1T ( = KEMC 41-098T  = JCM 17537T  = KACC 15558T).

Patulibacter medicamentivorans sp. nov., isolated from activated sludge of a wastewater treatment plant

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2588-2593 ◽  
Author(s):  
Bárbara Almeida ◽  
Ivone Vaz-Moreira ◽  
Peter Schumann ◽  
Olga C. Nunes ◽  
Gilda Carvalho ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Sequence Analysis ◽  
Type Species ◽  
Gene Sequence ◽  
Treatment Plant ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis

A Gram-positive, aerobic, non-motile, non-endospore-forming rod-shaped bacterium with ibuprofen-degrading capacity, designated strain I11T, was isolated from activated sludge from a wastewater treatment plant. The major respiratory quinone was demethylmenaquinone DMK-7, C18 : 1 cis9 was the predominant fatty acid, phosphatidylglycerol was the predominant polar lipid, the cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid and the G+C content of the genomic DNA was 74.1 mol%. On the basis of 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain I11T were Patulibacter ginsengiterrae CECT 7603T (96.8 % similarity), Patulibacter minatonensis DSM 18081T (96.6 %) and Patulibacter americanus DSM 16676T (96.6 %). Phenotypic characterization supports the inclusion of strain I11T within the genus Patulibacter (phylum Actinobacteria) . However, distinctive features and 16S rRNA gene sequence analysis suggest that is represents a novel species, for which the name Patulibacter medicamentivorans sp. nov. is proposed. The type strain is I11T ( = DSM 25962T = CECT 8141T).

Arthrobacter cheniae and Arthrobacter frigidicola sp. nov., isolated from a glacier

2021 ◽  
Vol 71 (12) ◽  
Author(s):  
Lei-Lei Yang ◽  
Hong-Can Liu ◽  
Qing Liu ◽  
Yu-Hua Xin
Keyword(s):  
Fatty Acids ◽  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Average Nucleotide Identity ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Unidentified Glycolipid

Two Gram-stain-positive, aerobic, rod-shaped, pink and light pink colony-forming bacteria, designated as Hz2T and MDT2-14T, respectively, were isolated from glacier cryoconite samples. Comparisons based on 16S rRNA gene sequences showed that strains Hz2T and MDT2-14T take Arthrobacter bussei KR32T and Arthrobacter zhaoguopingii J391T as their closest neighbours, respectively. The average nucleotide identity values between the two novel strains and their closest relatives were 83.56 and 93.06 %, respectively. The two strains contain MK-9(H2) as their predominant menaquinone. The polar lipids of both strains were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The major fatty acids of strain Hz2T were anteiso-C15 : 0, summed feature 3 (comprising C16 : 1  ω7c and/or C16 : 1  ω6c) and iso-C15 : 0, while the major fatty acids of strain MDT2-14T were anteiso-C15 : 0 and anteiso-C17 : 0. Based on these data, we propose two novel species, Arthrobacter cheniae sp. nov. (Hz2T = CGMCC 1.9262T=NBRC 113086T) and Arthrobacter frigidicola sp. nov. (MDT2-14T=CGMCC 1.9882T=NBRC 113089T).

scholarly journals Flavobacterium longum sp. nov. and Flavobacterium urocaniciphilum sp. nov., isolated from a wastewater treatment plant, and emended descriptions of Flavobacterium caeni and Flavobacterium terrigena

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1488-1494 ◽  
Author(s):  
Daichi Fujii ◽  
Fumiko Nagai ◽  
Yohei Watanabe ◽  
Yukio Shirasawa
Keyword(s):  
Wastewater Treatment ◽  
16S Rrna ◽  
Type Strain ◽  
Type Species ◽  
Gene Sequence ◽  
Treatment Plant ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Two Gram-staining-negative, strictly aerobic, non-endospore-forming, non-motile, rod-shaped bacteria, designated strains YIT 12745T and YIT 12746T, were isolated from sludge from a wastewater treatment plant. 16S rRNA gene sequence analyses indicated that these strains belonged to the genus Flavobacterium . In these analyses, strains YIT 12745T and YIT 12746T were most closely related to the type strains of Flavobacterium caeni and Flavobacterium terrigena , with 16S rRNA gene sequence similarity values of 94.9 % and 96.2 %, respectively. For both novel strains, menaquinone (MK-6) was the only respiratory quinone. The major fatty acids of strain YIT 12745T were iso-C15 : 1 G (14.4 %), iso-C16 : 0 (13.2 %), C15 : 0 (12.9 %), iso-C15 : 0 (12.9 %) and iso-C17 : 0 3-OH (11.5 %). Those of strain YIT 12746T were iso-C15 : 0 (21.5 %), iso-C16 : 0 (13.3 %), C15 : 0 (12.0 %) and iso-C15 : 1 G (11.9 %). The genomic DNA G+C contents of strains YIT 12745T and YIT 12746T were 48.7 and 30.9 mol%, respectively. From their differential phenotypic and phylogenetic characteristics, these strains are considered to represent two novel species of the genus Flavobacterium , for which the names Flavobacterium longum sp. nov. (type strain YIT 12745T = JCM 19141T = DSM 27077T) and Flavobacterium urocaniciphilum sp. nov. (type strain YIT 12746T = JCM 19142T = DSM 27078T) are proposed. Emended descriptions of Flavobacterium caeni and Flavobacterium terrigena are also proposed.

scholarly journals Reclassification of Desulfobacterium anilini as Desulfatiglans anilini comb. nov. within Desulfatiglans gen. nov., and description of a 4-chlorophenol-degrading sulfate-reducing bacterium, Desulfatiglans parachlorophenolica sp. nov.

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3081-3086 ◽  
Author(s):  
Daisuke Suzuki ◽  
Zhiling Li ◽  
Xinxin Cui ◽  
Chunfung Zhang ◽  
Arata Katayama
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Electron Acceptors ◽  
Electron Donors ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Content Type ◽  
Sulfate Reducing ◽  
Link Type

A strictly anaerobic, mesophilic, sulfate-reducing bacterial strain (DST), isolated from river sediment contaminated with volatile organic compounds, was characterized phenotypically and phylogenetically. Cells were Gram-reaction-negative, non-motile short rods. For growth, optimum NaCl concentration was 0.9 g l−1, optimum temperature was 30 °C and optimum pH was 7.2. Strain DST utilized phenol, benzoate, 4-hydroxybenzoate, 4-methylphenol, 4-chlorophenol, acetate, butyrate and pyruvate as electron donors for sulfate reduction. Electron donors were completely oxidized. Strain DST did not utilize sulfite, thiosulfate or nitrate as electron acceptors. The genomic DNA G+C content of strain DST was 58.9 mol%. Major cellular fatty acids were iso-C14 : 0, anteiso-C15 : 0 and C18 : 1ω7c. Phylogenetic analyses based on the 16S rRNA gene indicated its closest relatives were strains of Desulfobacterium anilini (about 98–99 % sequence similarity) but the DNA–DNA hybridization value with Desulfobacterium anilini Ani1T was around 40 %. Although strain DST and its relatives shared most phenotypic and chemotaxonomic characteristics, the utilization of 4-chlorophenol, the range of electron acceptors and the optimum growth conditions differed. Strain DST is closely related to strains of Desulfobacterium anilini , but constitutes a different species within the genus. Based on phylogeny, phenotypic characteristics and chemotaxonomic characteristics, strain DST and Desulfobacterium anilini were clearly different from strains of other species of the genus Desulfobacterium . We thus propose the reclassification of Desulfobacterium anilini within a new genus, Desulfatiglans gen. nov., as Desulfatiglans anilini comb. nov. We also propose Desulfatiglans parachlorophenolica sp. nov. to accommodate strain DST. The type strain is DST ( = JCM 19179T = DSM 27197T).

scholarly journals Desulfobotulus mexicanus sp. nov., a novel sulfate-reducing bacterium isolated from the sediment of an alkaline crater lake in Mexico

2020 ◽  
Vol 70 (5) ◽  
pp. 3219-3225 ◽  
Author(s):  
Maria Fernanda Pérez-Bernal ◽  
Elcia M. S. Brito ◽  
Manon Bartoli ◽  
Johanne Aubé ◽  
Bernard Ollivier ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Type Species ◽  
Crater Lake ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Terminal Electron Acceptor ◽  
Content Type ◽  
Sulfate Reducing ◽  
Link Type ◽  
Optimum Ph

A novel Gram-negative, non-spore-forming, vibrio-shaped, anaerobic, alkaliphilic, sulfate-reducing bacterium, designated strain PAR22NT, was isolated from sediment samples collected at an alkaline crater lake in Guanajuato (Mexico). Strain PAR22NT grew at temperatures between 15 and 37 °C (optimum, 32 °C), at pH between pH 8.3 and 10.1 (optimum, pH 9.0–9.6), and in the presence of NaCl up to 10 %. Pyruvate, 2-methylbutyrate and fatty acids (4–18 carbon atoms) were used as electron donors in the presence of sulfate as a terminal electron acceptor and were incompletely oxidized to acetate and CO2. Besides sulfate, both sulfite and elemental sulfur were also used as terminal electron acceptors and were reduced to sulfide. The predominant fatty acids were summed feature 10 (C18 : 1  ω7c and/or C18 : 1 ω9t and/or C18 : 1 ω12t), C18 : 1  ω9c and C16 : 0. The genome size of strain PAR22NT was 3.8 Mb including 3391 predicted genes. The genomic DNA G+C content was 49.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that it belongs to the genus Desulfobotulus within the class Deltaproteobacteria . Its closest phylogenetic relatives are Desulfobotulus alkaliphilus (98.4 % similarity) and Desulfobotulus sapovorans (97.9 % similarity). Based on phylogenetic, phenotypic and chemotaxonomic characteristics, we propose that the isolate represents a novel species of the genus Desulfobotulus with the name Desulfobotulus mexicanus sp. nov. The type strain is PAR22NT (=DSM 105758T=JCM 32146T).

scholarly journals Gordonia crocea sp. nov. and Gordonia spumicola sp. nov. isolated from sludge of a wastewater treatment plant

2020 ◽  
Vol 70 (6) ◽  
pp. 3718-3723 ◽  
Author(s):  
Tomohiko Tamura ◽  
Satomi Saito ◽  
Moriyuki Hamada ◽  
Yingqian Kang ◽  
Yasutaka Hoshino ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Treatment Plant ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Two novel actinobacteria, designated NBRC 107696T and NBRC 107697T, were isolated from sludge samples from a wastewater treatment plant and their taxonomic positions were investigated by a polyphasic approach. The cells of the strains were aerobic, rod-shaped, non-motile and non-endospore-forming. The strains contained glutamic acid, alanine and meso-diaminopimelic acid in the peptidoglycan. Galactose and arabinose were detected as cell-wall sugars. The predominant menaquinone was identified as MK-9(H2) and the major fatty acids were C16  :  0, C18 : 1ω9c and C16 : 1ω7c. The DNA G+C contents of NBRC 107696T and NBRC 107697T were 68.07 and 68.99 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that NBRC 107696T and NBRC 107697T were a clade with members of the genus Gordonia . The highest 16S rRNA gene sequence similarity values were obtained with Gordonia araii IFM 10211T (98.9 %) for NBRC 107697T, and Gordonia malaquae IMMIB WWCC-22T, Gordonia neofelifaecis AD-6T and Gordonia humi CC-12301T (98.1 %) for NBRC 107696T, respectively. The digital DNA–DNA relatedness data coupled with the combination of genotypic and phenotypic data indicated that the two strains are representatives of two novel separate species. The names proposed to accommodate these two strains are Gordonia spumicola sp. nov. and Gordonia crocea sp. nov., and the type strains are NBRC 107696T (=IFM 10067T=TBRC 11239T) and NBRC 107697T (=IFM 10881T=TBRC 11240T), respectively.

scholarly journals Pseudodesulfovibrio mercurii sp. nov., a mercury-methylating bacterium isolated from sediment

2019 ◽  
Vol 71 (3) ◽  
Author(s):  
Cynthia C. Gilmour ◽  
Ally Bullock Soren ◽  
Caitlin M. Gionfriddo ◽  
Mircea Podar ◽  
Judy D. Wall ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Type Species ◽  
Desulfovibrio Desulfuricans ◽  
Optimal Growth ◽  
Short Chain Fatty Acids ◽  
16S Rrna Sequence ◽  
Separate Species ◽  
Content Type ◽  
Sulfate Reducing ◽  
Link Type

The sulfate-reducing, mercury-methylating strain ND132T was isolated from the brackish anaerobic bottom sediments of Chesapeake Bay, USA. Capable of high levels of mercury (Hg) methylation, ND132T has been widely used as a model strain to study the process and to determine the genetic basis of Hg methylation. Originally called Desulfovibrio desulfuricans ND132T on the basis of an early partial 16S rRNA sequence, the strain has never been formally described. Phylogenetic and physiological traits place this strain within the genus Pseudodesulfovibrio, in the recently reclassified phylum Desulfobacterota (formerly Deltaproteobacteria ). ND132T is most closely related to Pseudodesulfovibrio hydrargyri BerOc1T and Pseudodesulfovibrio indicus J2T. Analysis of average nucleotide identity (ANI) of whole-genome sequences showed roughly 88 % ANI between P. hydrargyri BerOc1T and ND132T, and 84 % similarity between ND132T and P. indicus J2T. These cut-off scores <95 %, along with a multi-gene phylogenetic analysis of members of the family Desulfovibrionacea, and differences in physiology indicate that all three strains represent separate species. The Gram-stain-negative cells are vibrio-shaped, motile and not sporulated. ND132T is a salt-tolerant mesophile with optimal growth in the laboratory at 32 °C, 2 % salinity, and pH 7.8. The DNA G+C content of the genomic DNA is 65.2 %. It is an incomplete oxidizer of short chain fatty acids, using lactate, pyruvate and fumarate with sulfate or sulfite as the terminal electron acceptors. ND132T can respire fumarate using pyruvate as an electron donor. The major fatty acids are iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0, iso-C17 : 1ω9c and anteiso-C17 : 0. We propose the classification of strain ND132T (DSM 110689, ATCC TSD-224) as the type strain Pseudodesulfovibrio mercurii sp. nov.

scholarly journals Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Oribacterium

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2642-2649 ◽  
Author(s):  
Maria V. Sizova ◽  
Paul A. Muller ◽  
David Stancyk ◽  
Nicolai S. Panikov ◽  
Manolis Mandalakis ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Type Species ◽  
Sequence Similarity ◽  
Anaerobic Bacteria ◽  
Distinct Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Content Type ◽  
Link Type

Three strictly anaerobic, Gram-positive, non-spore-forming, rod-shaped, motile bacteria, designated strains ACB1T, ACB7T and ACB8, were isolated from human subgingival dental plaque. All strains required yeast extract for growth. Strains ACB1T and ACB8 were able to grow on glucose, lactose, maltose, maltodextrin and raffinose; strain ACB7T grew weakly on sucrose only. The growth temperature range was 30–42 °C with optimum growth at 37 °C. Major metabolic fermentation end products of strain ACB1T were acetate and lactate; the only product of strains ACB7T and ACB8 was acetate. Major fatty acids of strain ACB1T were C14 : 0, C16 : 0, C16 : 1ω7c dimethyl aldehyde (DMA) and C18 : 1ω7c DMA. Major fatty acids of strain ACB7T were C12 : 0, C14 : 0, C16 : 0, C16 : 1ω7c and C16 : 1ω7c DMA. The hydrolysate of the peptidoglycan contained meso-diaminopimelic acid, indicating peptidoglycan type A1γ. Genomic DNA G+C content varied from 42 to 43.3 % between strains. According to 16S rRNA gene sequence phylogeny, strains ACB1T, ACB8 and ACB7T formed two separate branches within the genus Oribacterium , with 98.1–98.6 % sequence similarity to the type strain of the type species, Oribacterium sinus . Predicted DNA–DNA hybridization values between strains ACB1T, ACB8, ACB7T and O. sinus F0268 were <70 %. Based on distinct genotypic and phenotypic characteristics, strains ACB1T and ACB8, and strain ACB7T are considered to represent two distinct species of the genus Oribacterium , for which the names Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov. are proposed. The type strains are ACB1T ( = DSM 24637T = HM-481T = ATCC BAA-2638T) and ACB7T ( = DSM 24638T = HM-482T = ATCC BAA-2639T), respectively.

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