scholarly journals Actinomyces dentalis sp. nov., from a human dental abscess

2005 ◽  
Vol 55 (1) ◽  
pp. 427-431 ◽  
Author(s):  
Val Hall ◽  
Matthew D. Collins ◽  
Paul A. Lawson ◽  
Enevold Falsen ◽  
Brian I. Duerden
Keyword(s):  
Novel Species ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Dental Abscess ◽  
Unknown Species ◽  
Sequencing Studies ◽  
Molecular Phylogenetic ◽  
Rrna Gene Sequencing ◽  
Biochemical Testing

A previously undescribed filamentous, beaded, Gram-positive, rod-shaped bacterium was isolated from pus of a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a distinct subline within the genus Actinomyces, clustering within a group of species that includes Actinomyces bovis, the type species of the genus. Sequence divergence values of >8 % with other recognized species within this phylogenetic group clearly demonstrated that the organism represents a hitherto unknown species. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unidentified organism recovered from a dental abscess be classified as a novel species, Actinomyces dentalis sp. nov. The type strain is R18165T (=CCUG 48064T=CIP 108337T).

scholarly journals Helcococcus sueciensis sp. nov., isolated from a human wound

2004 ◽  
Vol 54 (5) ◽  
pp. 1557-1560 ◽  
Author(s):  
Matthew D. Collins ◽  
Enevold Falsen ◽  
Kit Brownlee ◽  
Paul A. Lawson
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Taxonomic Study ◽  
Sequencing Studies ◽  
Molecular Phylogenetic ◽  
Rrna Gene Sequencing ◽  
Biochemical Testing

A previously undescribed, Gram-positive, catalase-negative, coccus-shaped organism that originated from a human wound was subjected to taxonomic study. On the basis of its cellular morphology and the results of biochemical testing, the unknown organism was identified tentatively as a member of the genus Helcococcus, but it did not correspond to either of the two recognized species of this genus. Comparative 16S rRNA gene sequencing studies confirmed that the bacterium was associated phylogenetically with the genus Helcococcus, with the unidentified organism forming a hitherto unknown subline within the genus. On the basis of biochemical, molecular chemical and molecular phylogenetic evidence, it is proposed that the unknown organism that was recovered from a human wound should be classified as a novel species of the genus Helcococcus, namely Helcococcus sueciensis sp. nov. The type strain is CCUG 47334T (=CIP 108183T).

scholarly journals Bacteroides coprosuis sp. nov., isolated from swine-manure storage pits

2005 ◽  
Vol 55 (6) ◽  
pp. 2515-2518 ◽  
Author(s):  
Terence R. Whitehead ◽  
Michael A. Cotta ◽  
Matthew D. Collins ◽  
Enevold Falsen ◽  
Paul A. Lawson
Keyword(s):  
Novel Species ◽  
Molecular Genetic ◽  
Swine Manure ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Sequencing Studies ◽  
Manure Storage ◽  
Rrna Gene Sequencing ◽  
Molecular Genetic Evidence

Two Gram-negative, anaerobic, non-spore-forming, rod-shaped organisms were isolated from a swine-manure storage pit. Based on morphological and biochemical criteria, the strains were tentatively identified as belonging to the genus Bacteroides but they did not appear to correspond to any recognized species of the genus. Comparative 16S rRNA gene sequencing studies showed that the strains were related closely to each other and confirmed their placement in the genus Bacteroides, but sequence divergence values of >10 % from reference Bacteroides species demonstrated that the organisms from manure represent a novel species. Based on biochemical criteria and molecular genetic evidence, it is proposed that the unknown isolates from manure be assigned to a novel species of the genus Bacteroides, as Bacteroides coprosuis sp. nov. The type strain is PC139T (=CCUG 50528T=NRRL B-41113T).

scholarly journals Atopococcus tabaci gen. nov., sp. nov., a novel Gram-positive, catalase-negative, coccus-shaped bacterium isolated from tobacco

2005 ◽  
Vol 55 (4) ◽  
pp. 1693-1696 ◽  
Author(s):  
Matthew D. Collins ◽  
Anna Wiernik ◽  
Enevold Falsen ◽  
Paul A. Lawson
Keyword(s):  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Gram Positive ◽  
Sequencing Studies ◽  
Molecular Phylogenetic ◽  
A Cell ◽  
Taxonomic Methods ◽  
Rrna Gene Sequencing

A novel Gram-positive, aerobic, catalase-negative, coccus-shaped organism originating from tobacco was characterized using phenotypic and molecular taxonomic methods. The organism contained a cell wall murein based on l-lysine (variation A4α, type l-lysine–l-glutamic acid), synthesized long-chain cellular fatty acids of the straight-chain saturated and monounsaturated types (with C16 : 1 ω9, C16 : 0 and C18 : 1 ω9 predominating) and possessed a DNA G+C content of 46 mol%. Based on morphological, biochemical and chemical characteristics, the coccus-shaped organism did not conform to any presently recognized taxon. Comparative 16S rRNA gene sequencing studies confirmed the distinctiveness of the unknown coccus, with the bacterium displaying sequence divergence values of greater than 7 % with other recognized Gram-positive taxa. Treeing analysis reinforced its distinctiveness, with the unidentified organism forming a relatively long subline branching at the periphery of an rRNA gene sequence cluster which encompasses the genera Alloiococcus, Allofustis, Alkalibacterium, Atopostipes, Dolosigranulum and Marinilactibacillus. Based on phenotypic and molecular phylogenetic evidence, it is proposed that the unknown organism from tobacco be classified as a new genus and species, Atopococcus tabaci gen. nov., sp. nov. The type strain of Atopococcus tabaci is CCUG 48253T (=CIP 108502T).

scholarly journals Suttonella ornithocola sp. nov., from birds of the tit families, and emended description of the genus Suttonella

2005 ◽  
Vol 55 (6) ◽  
pp. 2269-2272 ◽  
Author(s):  
Geoffrey Foster ◽  
Henry Malnick ◽  
Paul A. Lawson ◽  
James Kirkwood ◽  
Shaheed K. MacGregor ◽  
...  
Keyword(s):  
Novel Species ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Phylogenetic Studies ◽  
Coal Tit ◽  
The Family ◽  
Emended Description ◽  
Sequencing Studies ◽  
Rrna Gene Sequencing

Phenotypic and phylogenetic studies were performed on three strains of Gram-negative, rod-shaped organisms recovered from dead birds of the tit families (blue tit, coal tit and long-tailed tit). Morphological, cultural and biochemical studies indicated that the organisms were related to the family Cardiobacteriaceae in the gamma-subclass of the Proteobacteria. Comparative 16S rRNA gene sequencing studies confirmed these findings and demonstrated that the bacterium represents a hitherto unknown subline within this family. The closest phylogenetic relative of the strains isolated from the birds was found to be Suttonella indologenes, although a sequence divergence of approximately 5 % demonstrated that the unknown bacterium represented a novel species. On the basis of the results of the phylogenetic analysis and phenotypic criteria, it is proposed that the bacteria recovered from the diseased birds represent a novel species, Suttonella ornithocola sp. nov., with strain B6/99/2T (=CCUG 49457T=NCTC 13337T) as the type strain.

scholarly journals Streptococcus halichoeri sp. nov., isolated from grey seals (Halichoerus grypus)

2004 ◽  
Vol 54 (5) ◽  
pp. 1753-1756 ◽  
Author(s):  
Paul A. Lawson ◽  
Geoffrey Foster ◽  
Enevold Falsen ◽  
Nick Davison ◽  
Matthew D. Collins
Keyword(s):  
Novel Species ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Halichoerus Grypus ◽  
Rrna Gene ◽  
Phylogenetic Studies ◽  
Sds Page ◽  
Sequencing Studies ◽  
Streptococcal Species ◽  
Rrna Gene Sequencing

Phenotypic and phylogenetic studies were performed on six unidentified, Gram-positive, catalase-negative, chain-forming Streptococcus-like organisms recovered from grey seals. Biochemically the six strains were highly related to each other, but they did not appear to correspond to any recognized species of the genus Streptococcus. Comparative 16S rRNA gene sequencing studies confirmed that phylogenetically the strains were members of the genus Streptococcus, but sequence divergence values of greater than 3 % compared with reference streptococcal species demonstrated that the organisms from seals represent a novel species. SDS-PAGE analysis of whole-cell proteins confirmed the phenotypic distinctiveness of the seal organisms. Based on biochemical criteria and molecular chemical and genetic evidence, it is proposed that the unknown organism from seals be classified as a novel species, Streptococcus halichoeri sp. nov., the type strain of which is CCUG 48324T (=CIP 108195T).

scholarly journals Streptococcus marimammalium sp. nov., isolated from seals

2005 ◽  
Vol 55 (1) ◽  
pp. 271-274 ◽  
Author(s):  
Paul A. Lawson ◽  
Geoffrey Foster ◽  
Enevold Falsen ◽  
Matthew D. Collins
Keyword(s):  
Novel Species ◽  
Molecular Genetic ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Sequencing Studies ◽  
Streptococcal Species ◽  
Taxonomic Methods ◽  
Rrna Gene Sequencing ◽  
Molecular Genetic Evidence

Two strains of an unidentified, Gram-positive, catalase-negative, chain-forming, coccus-shaped organism recovered from seals were characterized using phenotypic and molecular taxonomic methods. Based on morphological and biochemical criteria the strains were tentatively identified as streptococci but they did not appear to correspond to any recognized species of the genus Streptococcus. Comparative 16S rRNA gene sequencing studies showed that the strains were closely related to each other and confirmed their placement in the genus Streptococcus. Sequence divergence values of >5 % with reference streptococcal species demonstrated the organisms from seals represent a novel species. SDS-PAGE analysis of whole-cell proteins confirmed that the two organisms were closely related to each other but were different from all currently defined streptococcal species. Based on biochemical criteria, molecular chemical and molecular genetic evidence, it is proposed that the unknown isolates from seals be assigned to a novel species of the genus Streptococcus, Streptococcus marimammalium sp. nov. The type strain is M54/01/1T (=CCUG 48494T=CIP 108309T).

scholarly journals Sutterella stercoricanis sp. nov., isolated from canine faeces

2004 ◽  
Vol 54 (5) ◽  
pp. 1581-1584 ◽  
Author(s):  
Hazel L. Greetham ◽  
Matthew D. Collins ◽  
Glenn R. Gibson ◽  
Catriona Giffard ◽  
Enevold Falsen ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Molecular Genetic ◽  
Gene Sequencing ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Anaerobic Conditions ◽  
Genetic Studies ◽  
Rrna Gene Sequencing

Morphological, biochemical and molecular genetic studies were carried out on an unknown non-spore-forming, Gram-negative, rod-shaped bacterium which was isolated from dog faeces. The bacterium grew under anaerobic conditions, was asaccharolytic, resistant to 20 % (v/v) bile and was oxidase- and urease-negative. Phylogenetic analysis based on comparative 16S rRNA gene sequencing showed that the unidentified bacterium clustered with Sutterella wadsworthensis, although a sequence divergence of >5 % indicated that the bacterium from dog faeces represented a previously unrecognized subline within the genus. On the basis of the presented findings, a novel species, Sutterella stercoricanis sp. nov., is described. The type strain of Sutterella stercoricanis is 5BAC4T (=CCUG 47620T=CIP 108024T).

scholarly journals Streptococcus dentapri sp. nov., isolated from the wild boar oral cavity

2010 ◽  
Vol 60 (4) ◽  
pp. 820-823 ◽  
Author(s):  
Kazuko Takada ◽  
Kazuhiko Hayashi ◽  
Yutaka Sato ◽  
Masatomo Hirasawa
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phylogenetic Studies ◽  
Streptococcus Equi ◽  
Sequencing Studies ◽  
Streptococcal Species ◽  
Rrna Gene Sequencing

Four Gram-stain-positive, catalase-negative, coccoid-shaped isolates were obtained from the oral cavities of wild boars and characterized by phenotypic and phylogenetic studies. On the results of biochemical tests, the organisms were tentatively identified as a streptococcal species. Comparative 16S rRNA gene sequencing studies confirmed that the organisms are members of the genus Streptococcus, with Streptococcus equi subsp. equi ATCC 33398T as their closest phylogenetic relative (94.7 % similarity). DNA–DNA hybridization analysis showed that the isolates displayed less than 10 % relatedness to Streptococcus equi subsp. equi DSM 20561T. From the phylogenetic and phenotypic evidence, the four isolates represent a novel species of the genus Streptococcus, for which the name Streptococcus dentapri sp. nov. (type strain NUM 1529T =JCM 15752T =DSM 21999T) is proposed.

scholarly journals Corynebacterium caspium sp. nov., from a Caspian seal (Phoca caspica)

2004 ◽  
Vol 54 (3) ◽  
pp. 925-928 ◽  
Author(s):  
Matthew D. Collins ◽  
Lesley Hoyles ◽  
Geoffrey Foster ◽  
Enevold Falsen
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Sequencing ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Facultative Anaerobe ◽  
Phenotypic Differences ◽  
Rrna Gene Sequencing ◽  
Caspian Seal

A previously unknown Gram-positive, non-spore-forming, non-lipophilic, catalase-positive, irregular rod-shaped bacterium (M/106/00/5T) was isolated, in mixed culture, from the penis of a Caspian seal (Phoca caspica). The strain was a facultative anaerobe that was able to grow at 22 and 42 °C. Comparative 16S rRNA gene sequencing showed that the organism formed a hitherto unknown subline within the genus Corynebacterium. Sequence divergence values of more than 5 % from other described Corynebacterium species, together with phenotypic differences, showed that the unidentified bacterium represents a previously unrecognized member of this genus. On the basis of phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium isolated from a Caspian seal (strain M/106/00/5T=CCUG 44566T=CIP 107965T) be classified as the type strain of a novel species of the genus Corynebacterium, Corynebacterium caspium sp. nov.

scholarly journals Corynebacterium ciconiae sp. nov., isolated from the trachea of black storks (Ciconia nigra)

2004 ◽  
Vol 54 (6) ◽  
pp. 2191-2195 ◽  
Author(s):  
J. F. Fernández-Garayzábal ◽  
A. I. Vela ◽  
R. Egido ◽  
R. A. Hutson ◽  
M. P. Lanzarot ◽  
...  
Keyword(s):  
Novel Species ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Taxonomic Analysis ◽  
Sequencing Studies ◽  
Ciconia Nigra ◽  
Rrna Gene Sequencing ◽  
Close Relatives ◽  
Apparently Healthy

Eight unidentified Gram-positive, rod-shaped organisms were recovered from the tracheas of apparently healthy black storks (Ciconia nigra) and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria the isolates were tentatively assigned to the genus Corynebacterium, although three of the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequencing studies demonstrated that all of the isolates were phylogenetically members of the genus Corynebacterium. Five strains were genotypically identified as representing Corynebacterium falsenii, whereas the remaining three strains represented a hitherto unknown subline, associated with a small subcluster of species that includes Corynebacterium mastitidis and its close relatives. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from black storks represent a novel species within the genus Corynebacterium, for which the Corynebacterium ciconiae sp. nov. is proposed. The type strain is CECT 5779T (=BS13T=CCUG 47525T).

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