scholarly journals Natronorubrum aibiense sp. nov., an extremely halophilic archaeon isolated from Aibi salt lake in Xin-Jiang, China, and emended description of the genus Natronorubrum

2006 ◽  
Vol 56 (7) ◽  
pp. 1515-1517 ◽  
Author(s):  
Heng-Lin Cui ◽  
Dilbr Tohty ◽  
Jie Feng ◽  
Pei-Jin Zhou ◽  
Shuang-Jiang Liu
Keyword(s):  
Gene Sequence ◽  
Agar Medium ◽  
Novel Species ◽  
Salt Lake ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Optimum Ph ◽  
Emended Description ◽  
Extremely Halophilic Archaeon ◽  
Sequence Similarities

An extremely halophilic archaeon (strain 7-3T) was isolated from Aibi salt lake in Xin-Jiang, China. It formed small (<1.0 mm), red, elevated colonies on salt-milk agar medium. Strain 7-3T was able to grow at pH 6.5–9.5 (optimum pH 8.0) and at 20–50 °C (optimum 45 °C). Analysis of its 16S rRNA gene sequence indicated that strain 7-3T was phylogenetically related to members of the genus Natronorubrum, with sequence similarities of 97.0 % to Natronorubrum bangense A33T and 95.2 % to Natronorubrum tibetense GA33T. The G+C content of its DNA was 61.2 mol% (T m). Levels of DNA–DNA relatedness to Nrr. bangense and Nrr. tibetense were 45 and 37 %, respectively. It is concluded that strain 7-3T (=CGMCC 1.4299T=JCM 13488T) should be classified as the type strain of a novel species of the genus Natronorubrum, for which the name Natronorubrum aibiense sp. nov. is proposed. Based on the properties of Nrr. aibiense given here and of those of Nrr. bangense and Nrr. tibetense described previously, an emended description of the genus Natronorubrum is presented.

scholarly journals Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern

2007 ◽  
Vol 57 (10) ◽  
pp. 2204-2206 ◽  
Author(s):  
Heng-Lin Cui ◽  
Ze-Ying Lin ◽  
Ying Dong ◽  
Pei-Jin Zhou ◽  
Shuang-Jiang Liu
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Halophilic Archaeon ◽  
Marine Solar Saltern ◽  
Gene Sequence Analysis ◽  
Extremely Halophilic Archaeon ◽  
Sequence Similarities

An extremely halophilic archaeon, strain Fa-1T, was isolated from a marine solar saltern in Fujian, China. Strain Fa-1T required Mg2+ and at least 2.0 M NaCl for growth. It was able to grow at pH 6.5–9.0 (optimally at pH 7.0–7.5) and at 20–55 °C (optimally at 37–42 °C). The major polar lipids of strain Fa-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl diether. On the basis of a 16S rRNA gene sequence analysis, strain Fa-1T was closely related to nine species of the genus Halorubrum, showing sequence similarities of 97.4–98.4 %. The G+C content of the DNA of strain Fa-1T is 64.9 mol% (T m). DNA–DNA hybridization values between strain Fa-1T and the most closely related members of the genus Halorubrum were below 51 %. On the basis of the data from this study, strain Fa-1T represents a novel species of the genus Halorubrum, for which the name Halorubrum litoreum sp. nov. is proposed. The type strain is Fa-1T (=CGMCC 1.5336T =JCM 13561T).

scholarly journals Spirosoma aerolatum sp. nov., isolated from a motor car air conditioning system

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 4003-4007 ◽  
Author(s):  
Dong-Uk Kim ◽  
Hyosun Lee ◽  
Song-Gun Kim ◽  
Jae-Hyung Ahn ◽  
So Yoon Park ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Gram Stain ◽  
Rrna Gene Sequence ◽  
Air Conditioning System ◽  
Optimum Ph ◽  
Sequence Similarities

A Gram-stain-negative, yellow-pigmented bacterial strain, designated PR1012KT, was isolated from a motor car evaporator core collected in Korea. Cells of the strain were facultatively anaerobic, non-spore-forming and rod-shaped. The strain grew at 10–40 °C (optimum, 25 °C), at pH 6.5–8.0 (optimum, pH 7.0–8.0) and in the presence of 0–1 % (w/v) NaCl. Phylogenetically, the strain was closely related to members of the genus Spirosoma (97.50–90.74 % 16S rRNA gene sequence similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099T (97.50 %). Its predominant fatty acids included summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω5c, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso B) and it had MK-7 as the major menaquinone. The polar lipids present included phosphatidylethanolamine, one unknown aminophospholipid, two unknown aminolipids and five unknown polar lipids. The DNA G+C content of this strain was 54 mol%. Based on phenotypic, genotypic and chemotaxonomic data, strain PR1012KT represents a novel species in the genus Spirosoma, for which the name Spirosoma aerolatum sp. nov. is proposed. The type strain is PR1012KT ( = KACC 17939T = NBRC 110794T).

scholarly journals Spirosoma oryzae sp. nov., isolated from rice soil and emended description of the genus Spirosoma

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3230-3234 ◽  
Author(s):  
Jae-Hyung Ahn ◽  
Hang-Yeon Weon ◽  
Soo-Jin Kim ◽  
Seung-Beom Hong ◽  
Soon-Ja Seok ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rice Soil ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Unknown Phospholipid ◽  
Sequence Similarities

A bacterial strain, designated RHs22T, was isolated from a soil sample cultivated with rice in the Suwon region of South Korea. The cells were aerobic, Gram-stain-negative, non-spore-forming, non-flagellated rods or occasionally filaments. The strain grew at 10–37 °C (optimum, 28–30 °C), at pH 5.0–10.0 (optimum, 7.0) and in the presence of 0–1 % (w/v) NaCl (optimum, 0 %). Phylogenetically, the strain was closely related to members of the genus Spirosoma , as its 16S rRNA gene sequence had similarity of 90.3–92.1 % with respect to those of members of the genus Spirosoma , showing the highest sequence similarity with Spirosoma panaciterrae DSM 21099T. Strain RHs22T revealed relatively low sequence similarities of less than 90 % with all the other species with validly published names. It contained MK-7 as the predominant menaquinone and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 1ω5c, iso-C15 : 0 and iso-C17 : 0 3-OH as the main fatty acids. The polar lipids of strain RHs22T were phosphatidylethanolamine, one unknown aminolipid, two unknown aminophospholipids, one unknown phospholipid and five unknown lipids. The DNA G+C content was 57.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data obtained in this study indicate that strain RHs22T represents a novel species of the genus Spirosoma , for which the name Spirosoma oryzae sp. nov. is proposed. The type strain is RHs22T ( = KACC 17324T = DSM 28354T). An emended description of the genus Spirosoma is also proposed.

scholarly journals Haloterrigena hispanica sp. nov., an extremely halophilic archaeon from Fuente de Piedra, southern Spain

2007 ◽  
Vol 57 (7) ◽  
pp. 1499-1503 ◽  
Author(s):  
Ida Romano ◽  
Annarita Poli ◽  
Ilaria Finore ◽  
F. Javier Huertas ◽  
Agata Gambacorta ◽  
...  
Keyword(s):  
Lipid Analysis ◽  
Novel Species ◽  
Salt Lake ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
16S Rrna Gene Sequences ◽  
Closely Related Species ◽  
Dna Reassociation ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon belonging to the order Halobacteriales was isolated from Fuente de Piedra salt lake, Spain. This strain, designated FP1T, was a pleomorphic coccoid, neutrophilic and required at least 15 % (w/v) NaCl for growth. Strain FP1T grew at 37–60 °C, with optimal growth at 50 °C. Mg2+ was not required, but growth was observed with up to 10 % (w/v) MgSO4. Polar lipid analysis revealed the presence of mannose-6-sulfate(1-2)-glucose glycerol diether as a major glycolipid. Both C20C20 and C20C25 core lipids were present. The genomic DNA G+C content was 62.0 mol%. Phylogenetic analysis based on comparison of 16S rRNA gene sequences demonstrated that the isolate was most closely related to species of the genus Haloterrigena. DNA–DNA reassociation values between strain FP1T and the most closely related species of the genus Haloterrigena (Haloterrigena thermotolerans, Haloterrigena saccharevitans and Haloterrigena limicola) were lower than 29 %. It is therefore considered that strain FP1T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena hispanica sp. nov. is proposed. The type strain is FP1T (=DSM 18328T=ATCC BAA-1310T).

scholarly journals Aneurinibacillus soli sp. nov., isolated from mountain soil

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3792-3797 ◽  
Author(s):  
Keun Chul Lee ◽  
Kwang Kyu Kim ◽  
Mi Kyung Eom ◽  
Jong-Shik Kim ◽  
Dae-Shin Kim ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Content Sharing ◽  
Link Type ◽  
Emended Description ◽  
Aneurinibacillus Aneurinilyticus ◽  
Sequence Similarities

A novel bacterial strain designated CB4T was isolated from soil from the Hallasan, Jeju, Korea. Strain CB4T was found to be strictly aerobic, Gram-stain-positive, rod-shaped, motile and formed creamy greyish colonies on nutrient agar. The major fatty acids were identified as iso-C15 : 0 and iso-C16 : 0, and the predominant isoprenoid quinone as MK-7. The cell-wall peptidoglycan contained glycine and alanine as the diagnostic amino acids and phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified aminophospholipid as the polar lipids. The genomic DNA G+C content of strain CB4T was 46.5 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain CB4T forms a deep branch within the genus Aneurinibacillus , sharing the highest level of sequence homology with Aneurinibacillus aneurinilyticus DSM 5562T (96.5 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain CB4T is considered to represent a novel species within the genus Aneurinibacillus , for which the name Aneurinibacillus soli sp. nov. is proposed. The type strain is CB4T ( = KCTC 33505T = CECT 8566T). An emended description of the genus Aneurinibacillus is also proposed.

scholarly journals Natronorubrum sulfidifaciens sp. nov., an extremely haloalkaliphilic archaeon isolated from Aiding salt lake in Xin-Jiang, China

2007 ◽  
Vol 57 (4) ◽  
pp. 738-740 ◽  
Author(s):  
Heng-Lin Cui ◽  
Dilbr Tohty ◽  
Hong-Can Liu ◽  
Shuang-Jiang Liu ◽  
Aharon Oren ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Salt Lake ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Ph Range ◽  
Type Strains ◽  
Sequence Similarities

An extremely haloalkaliphilic archaeon, strain AD2T, was isolated from Aiding salt lake in Xin-Jiang, China. Strain AD2T required at least 12 % NaCl for growth. MgCl2 was not required. The isolate was able to grow over a pH range of 8.0–10.0 and temperature range of 20–55 °C, with optimal growth at pH 8.7–9.2 and 44–47 °C. The major polar lipids of strain AD2T were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester; glycolipids were not detected. Analysis of its 16S rRNA gene sequence indicated that strain AD2T was phylogenetically related to members of the genus Natronorubrum, with sequence similarities to the type strains of Natronorubrum bangense, Natronorubrum tibetense and Natronorubrum aibiense of 97.1, 95.9 and 96.1 %, respectively. The G+C content of its DNA was 60.9 mol% (T m). Levels of DNA–DNA relatedness between strain AD2T and the type strains of Nrr. bangense, Nrr. tibetense and Nrr. aibiense were 49, 38 and 41 %, respectively. It was concluded that strain AD2T represents a novel species of the genus Natronorubrum, for which the name Natronorubrum sulfidifaciens sp. nov. is proposed. The type strain is AD2T (=CGMCC 1.6307T=JCM 14089T).

scholarly journals Fulvimonas yonginensis sp. nov., isolated from greenhouse soil, and emended description of the genus Fulvimonas

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2758-2762 ◽  
Author(s):  
Jae-Hyung Ahn ◽  
Soo-Jin Kim ◽  
Hang-Yeon Weon ◽  
Seung-Beom Hong ◽  
Soon-Ja Seok ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Greenhouse Soil ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Sequence Similarities

A novel strain, designated 5GHs31-2T, was isolated from a greenhouse soil sample collected from Yongin city, South Korea. Cells of strain 5GHs31-2T were Gram-stain-negative, rod-shaped, polar flagellated and yellow-pigmented. The isolate was aerobic, catalase-negative and oxidase-positive and grew optimally at 28–30 °C and pH 7.0. Strain 5GHs31-2T revealed the highest 16S rRNA gene sequence similarities with Fulvimonas soli LMG 19981T (97.6 %), Dyella thiooxydans ATSB10T (97.5 %) and Frateuria terrea VA24T (97.4 %). Furthermore, the neighbour-joining tree showed that strain 5GHs31-2T was a representative of a member of the genus Fulvimonas . Strain 5GHs31-2T contained iso-C16 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C15 : 0 and iso-C17 : 0 as the major fatty acids, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an aminolipid as the main polar lipids and Q-8 as the predominant quinone. The genomic DNA G+C content of 5GHs31-2T was 73.0 mol%. According to DNA–DNA hybridization experiments, DNA–DNA relatedness values between strain 5GHs31-2T and its closest phylogenetic neighbours were below 70 %. Based on the taxonomic data, strain 5GHs31-2T represents a novel species of the genus Fulvimonas , for which the name Fulvimonas yonginensis sp. nov. is proposed. The type strain is 5GHs31-2T ( = KACC 16952T = DSM 28344T).

scholarly journals Halobacterium jilantaiense sp. nov., a halophilic archaeon isolated from a saline lake in Inner Mongolia, China

2006 ◽  
Vol 56 (10) ◽  
pp. 2353-2355 ◽  
Author(s):  
Yong Yang ◽  
Heng-Lin Cui ◽  
Pei-Jin Zhou ◽  
Shuang-Jiang Liu
Keyword(s):  
Inner Mongolia ◽  
Gene Sequence ◽  
Saline Lake ◽  
Novel Species ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Halobacterium Salinarum ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Rrna Gene Sequence

A novel halophilic archaeon, NG4T, was isolated from Jilantai salt lake in Inner Mongolia, China. The taxonomy of strain NG4T was studied by polyphasic methods. Strain NG4T grew at pH 5.5–8.5 and at a temperature of 22–55 °C. It was chemo-organotrophic, aerobic and required concentrations of 2.7–5.2 M NaCl and 0.05–0.3 M Mg2+ for growth. Cells were Gram-negative, slender rods. Colonies on agar plates containing 25 % (w/v) total salts were red, elevated and round. According to 16S rRNA gene sequence similarity, strain NG4T was phylogenetically related to Halobacterium salinarum DSM 3754T (98.2 %) and Halobacterium noricense A1T (97.3 %). The DNA G+C content was 64.2 mol%. DNA–DNA relatedness values with Hbt. salinarum DSM 3754T and Hbt. noricense A1T were 47 and 35 %, respectively. The polar lipids of strain NG4T consisted of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether, sulfated triglycosyl diether and sulfated tetraglycosyl diether. It was concluded that strain NG4T represents a novel species of the genus Halobacterium, for which the name Halobacterium jilantaiense sp. nov. is proposed. The type strain is NG4T (=CGMCC 1.5337T=JCM 13558T).

Halorientalis persicus sp. nov., an extremely halophilic archaeon isolated from a salt lake and emended description of the genus Halorientalis

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 940-944 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Ali Makhdoumi-Kakhki ◽  
Maliheh Mehrshad ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
Cathrin Spröer ◽  
...  
Keyword(s):  
Type Strain ◽  
Type Species ◽  
Salt Lake ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Content Type ◽  
Link Type ◽  
Brine Sample ◽  
Emended Description ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon, strain D108T, was isolated from a brine sample of Aran-Bidgol salt lake in Iran. The novel strain was cream-pigmented, motile, pleomorphic rod-shaped and required at least 2.5 M NaCl but not MgCl2 for growth. Optimal growth was achieved with 4.3 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 40 °C, respectively, and the strain was able to grow over a pH range of 6.5 to 9.0, and a temperature range of 30 to 50 °C. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain D108T clustered with the type strain of the sole species of the genus Halorientalis , Halorientalis regularis TNN28T, with a sequence similarity of 98.8 %. The polar lipid pattern of strain D108T consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, one phosphoglycolipid and two glycolipids. The only quinone present was MK-8(II-H2). The DNA G+C content of strain D108T was 62.8 mol%. DNA–DNA hybridization studies (45 % with Halorientalis regularis IBRC-M 10760T), as well as biochemical and physiological characterization, allowed strain D108T to be differentiated from Halorientalis regularis . A novel species of the genus Halorientalis , Halorientalis persicus sp. nov., is therefore proposed to accommodate this strain. The type strain is D108T ( = IBRC-M 10043T = CECT 8375T). An emended description of the genus Halorientalis is also proposed.

scholarly journals Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004

2007 ◽  
Vol 57 (9) ◽  
pp. 1988-1994 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Seung Bum Kim ◽  
Kae Kyoung Kwon ◽  
Dong Sung Shin ◽  
Xuseong Luo ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Genotypic Characteristics ◽  
Sequence Similarities

A taxonomic study of a novel marine, heterotrophic, non-gliding, halotolerant and light-pink-pigmented bacterium was carried out using a polyphasic approach. 16S rRNA gene sequence analysis revealed that strain KMM 6241T is a member of the phylum Bacteroidetes. Strain KMM 6241T formed a cluster with the genera Algoriphagus, Chimaereicella and Hongiella with sequence similarities of 94.0–98.2 %. Hongiella ornithinivorans was the closest relative of the novel isolate. Comparative analysis of phenotypic, chemotaxonomic and genotypic characteristics of strain KMM 6241T and representatives of the genera Algoriphagus, Chimaereicella and Hongiella revealed many similar features. Consequently, phylogenetic evidence supported by phenotypic and genotypic similarities support the transfer of members of the genera Chimaereicella and Hongiella to the genus Algoriphagus and the establishment of a novel species, Algoriphagus vanfongensis sp. nov., with strain KMM 6241T (=DSM 17529T=KCTC 12716T) as the type strain.

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