scholarly journals Aneurinibacillus soli sp. nov., isolated from mountain soil

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3792-3797 ◽  
Author(s):  
Keun Chul Lee ◽  
Kwang Kyu Kim ◽  
Mi Kyung Eom ◽  
Jong-Shik Kim ◽  
Dae-Shin Kim ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Content Sharing ◽  
Link Type ◽  
Emended Description ◽  
Aneurinibacillus Aneurinilyticus ◽  
Sequence Similarities

A novel bacterial strain designated CB4T was isolated from soil from the Hallasan, Jeju, Korea. Strain CB4T was found to be strictly aerobic, Gram-stain-positive, rod-shaped, motile and formed creamy greyish colonies on nutrient agar. The major fatty acids were identified as iso-C15 : 0 and iso-C16 : 0, and the predominant isoprenoid quinone as MK-7. The cell-wall peptidoglycan contained glycine and alanine as the diagnostic amino acids and phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified aminophospholipid as the polar lipids. The genomic DNA G+C content of strain CB4T was 46.5 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain CB4T forms a deep branch within the genus Aneurinibacillus , sharing the highest level of sequence homology with Aneurinibacillus aneurinilyticus DSM 5562T (96.5 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain CB4T is considered to represent a novel species within the genus Aneurinibacillus , for which the name Aneurinibacillus soli sp. nov. is proposed. The type strain is CB4T ( = KCTC 33505T = CECT 8566T). An emended description of the genus Aneurinibacillus is also proposed.

scholarly journals Flaviflexus ciconiae sp. nov., isolated from the faeces of the oriental stork, Ciconia boyciana

2020 ◽  
Vol 70 (10) ◽  
pp. 5439-5444 ◽  
Author(s):  
Jae-Yun Lee ◽  
Woorim Kang ◽  
Pil Soo Kim ◽  
So-Yeon Lee ◽  
Na-Ri Shin ◽  
...  
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Novel Species ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Link Type ◽  
Sequence Similarities

A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea. Optimal growth of strain H23T48T was observed at 30–37 °C, pH 8 and with 3 % (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus , with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively. Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1  ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids. The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids. The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine. The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %. Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus , for which the name Flaviflexus ciconiae sp. nov. is proposed. The type strain is H23T48T (=KCTC 49253T=JCM 33282T).

scholarly journals Spirosoma oryzae sp. nov., isolated from rice soil and emended description of the genus Spirosoma

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3230-3234 ◽  
Author(s):  
Jae-Hyung Ahn ◽  
Hang-Yeon Weon ◽  
Soo-Jin Kim ◽  
Seung-Beom Hong ◽  
Soon-Ja Seok ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rice Soil ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Unknown Phospholipid ◽  
Sequence Similarities

A bacterial strain, designated RHs22T, was isolated from a soil sample cultivated with rice in the Suwon region of South Korea. The cells were aerobic, Gram-stain-negative, non-spore-forming, non-flagellated rods or occasionally filaments. The strain grew at 10–37 °C (optimum, 28–30 °C), at pH 5.0–10.0 (optimum, 7.0) and in the presence of 0–1 % (w/v) NaCl (optimum, 0 %). Phylogenetically, the strain was closely related to members of the genus Spirosoma , as its 16S rRNA gene sequence had similarity of 90.3–92.1 % with respect to those of members of the genus Spirosoma , showing the highest sequence similarity with Spirosoma panaciterrae DSM 21099T. Strain RHs22T revealed relatively low sequence similarities of less than 90 % with all the other species with validly published names. It contained MK-7 as the predominant menaquinone and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 1ω5c, iso-C15 : 0 and iso-C17 : 0 3-OH as the main fatty acids. The polar lipids of strain RHs22T were phosphatidylethanolamine, one unknown aminolipid, two unknown aminophospholipids, one unknown phospholipid and five unknown lipids. The DNA G+C content was 57.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data obtained in this study indicate that strain RHs22T represents a novel species of the genus Spirosoma , for which the name Spirosoma oryzae sp. nov. is proposed. The type strain is RHs22T ( = KACC 17324T = DSM 28354T). An emended description of the genus Spirosoma is also proposed.

scholarly journals Fulvimonas yonginensis sp. nov., isolated from greenhouse soil, and emended description of the genus Fulvimonas

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2758-2762 ◽  
Author(s):  
Jae-Hyung Ahn ◽  
Soo-Jin Kim ◽  
Hang-Yeon Weon ◽  
Seung-Beom Hong ◽  
Soon-Ja Seok ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Greenhouse Soil ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Sequence Similarities

A novel strain, designated 5GHs31-2T, was isolated from a greenhouse soil sample collected from Yongin city, South Korea. Cells of strain 5GHs31-2T were Gram-stain-negative, rod-shaped, polar flagellated and yellow-pigmented. The isolate was aerobic, catalase-negative and oxidase-positive and grew optimally at 28–30 °C and pH 7.0. Strain 5GHs31-2T revealed the highest 16S rRNA gene sequence similarities with Fulvimonas soli LMG 19981T (97.6 %), Dyella thiooxydans ATSB10T (97.5 %) and Frateuria terrea VA24T (97.4 %). Furthermore, the neighbour-joining tree showed that strain 5GHs31-2T was a representative of a member of the genus Fulvimonas . Strain 5GHs31-2T contained iso-C16 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C15 : 0 and iso-C17 : 0 as the major fatty acids, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an aminolipid as the main polar lipids and Q-8 as the predominant quinone. The genomic DNA G+C content of 5GHs31-2T was 73.0 mol%. According to DNA–DNA hybridization experiments, DNA–DNA relatedness values between strain 5GHs31-2T and its closest phylogenetic neighbours were below 70 %. Based on the taxonomic data, strain 5GHs31-2T represents a novel species of the genus Fulvimonas , for which the name Fulvimonas yonginensis sp. nov. is proposed. The type strain is 5GHs31-2T ( = KACC 16952T = DSM 28344T).

scholarly journals Phenylobacterium kunshanense sp. nov., isolated from the sludge of a pesticide manufacturing factory

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 325-330 ◽  
Author(s):  
Cuiwei Chu ◽  
Cansheng Yuan ◽  
Xin Liu ◽  
Li Yao ◽  
Jianchun Zhu ◽  
...  
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Comparisons ◽  
Content Type ◽  
Link Type ◽  
Ubiquinone 10 ◽  
Sequence Similarities

A novel aerobic, Gram-stain-negative, motile bacterium, designated strain BUT-10T, was isolated from the sludge of a pesticide manufacturing factory in Kunshan, China. Cells were rod-shaped (0.4–0.45×0.9–1.4 µm) and colonies were white, circular with entire edges and had a smooth surface. The strain grew at 25–37 °C, at pH 6.0–8.0 and with 0–0.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BUT-10T was a member of the genus Phenylobacterium , and showed highest sequence similarities to Phenylobacterium muchangponense A8T (97.49 %), Phenylobacterium immobile DSM 1986T (97.14 %) and Phenylobacterium lituiforme FaiI3T (96.34 %). Major fatty acids (>5 %) were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The major isoprenoid quinone was ubiquinone-10. The DNA G+C content was 71.85 mol%. Strain BUT-10T showed low DNA–DNA relatedness with P. muchangponense A8T (15.7±2.9 %) and P. immobile DSM 1986T (12.8±1.1 %). On the basis of the phenotypic, phylogenetic and genotypic data, strain BUT-10T is considered to represent a novel species of the genus Phenylobacterium , for which the name Phenylobacterium kunshanense sp. nov. is proposed. The type strain is BUT-10T ( = CCTCC AB 2013085T = KCTC 42014T).

scholarly journals Gilvimarinus polysaccharolyticus sp. nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 562-569 ◽  
Author(s):  
Hong Cheng ◽  
Shun Zhang ◽  
Ying-Yi Huo ◽  
Xia-Wei Jiang ◽  
Xin-Qi Zhang ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Hydrolysis Of ◽  
Sequence Similarities

A taxonomic study was carried out on strain YN3T, which was isolated from a seaweed sample taken from the coast of Weihai, China. The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0–10.0 and 4–32 °C in the presence of 0–9.0 % (w/v) NaCl. Strain YN3T was positive for the hydrolysis of polysaccharides, such as agar, starch and xylan. The predominant respiratory quinone was ubiquinone-8. The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and two unidentified glycolipids. The genomic DNA G+C content was 49.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YN3T should be assigned to the genus Gilvimarinus . ‘Gilvimarinus agarilyticus’ KCTC 23325 and Gilvimarinus chinensis QM42T had the closest phylogenetic relationship to strain YN3T, and showed 97.9 % and 95.8 % sequence similarities, respectively. On the basis of phenotypic, chemotaxonomic and genotypic data and DNA–DNA hybridization studies, we propose that strain YN3T represents a novel species of the genus Gilvimarinus , for which the name Gilvimarinus polysaccharolyticus sp. nov. is proposed. The type strain is YN3T ( = KCTC 32438T = JCM 19198T). An emended description of the genus Gilvimarinus is also presented.

Haloferula chungangensis sp. nov., isolated from marine sediment

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 19-24 ◽  
Author(s):  
Hyeonji Kang ◽  
Jitsopin Traiwan ◽  
Veeraya Weerawongwiwat ◽  
Min Young Jung ◽  
Ji Hoon Jeong ◽  
...  
Keyword(s):  
Marine Sediment ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Distinct Lineage

A Gram-stain-negative, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated CAU 1074T, was isolated from marine sediment and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1074T grew optimally at 30 °C and pH 6.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1074T formed a distinct lineage within the genus Haloferula and was most closely related to Haloferula harenae KCTC 22198T (96.0 % similarity). Strain CAU 1074T contained MK-9 as the major isoprenoid quinone, and iso-C14 : 0, C16 : 1ω9c and C16 : 0 as the major fatty acids. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major whole-cell sugars were glucose, xylose, mannose and ribose. The polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminoglycolipid and two unidentified phospholipids. The DNA G+C content of the strain was 64.0 mol%. On the basis of phenotypic and chemotaxonomic data, and phylogenetic inference, strain CAU 1074T should be classified as a member of a novel species in the genus Haloferula , for which the name Haloferula chungangensis sp. nov. is proposed; the type strain is CAU 1074T ( = KCTC 23578T = CCUG 61920T). An emended description of the genus Haloferula is also provided.

Aquimarina agarilytica sp. nov., an agarolytic species isolated from a red alga

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 869-873 ◽  
Author(s):  
Bokun Lin ◽  
Guoyong Lu ◽  
Yandan Zheng ◽  
Wei Xie ◽  
Shengkang Li ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Red Alga ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
The 16S Rrna Gene

A novel yellow-pigmented, agarolytic bacterial strain, designated ZC1T, was isolated from the surface of the marine red alga Porphyra haitanensis collected near Nan Ao Island, Guangdong province, China. The isolate was Gram-stain-negative, strictly aerobic and rod-shaped and displayed β-galactosidase, alkaline phosphatase, catalase and oxidase activities. The predominant cellular fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) and iso-C17 : 0 3-OH. The major menaquinone was menaquinone 6 (MK-6). The DNA G+C content was 32.8 mol%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain ZC1T was closely related to members of the genus Aquimarina in the family Flavobacteriaceae , phylum Bacteroidetes . Based on phylogenetic and phenotypic evidence, strain ZC1T ( = CCTCC AB 2010229T  = NBRC 107695T) represents the type strain of a novel species in the genus Aquimarina , for which the name Aquimarina agarilytica sp. nov. is proposed.

Kaistia defluvii sp. nov., isolated from river sediment

2012 ◽  
Vol 62 (Pt_12) ◽  
pp. 2878-2882 ◽  
Author(s):  
Long Jin ◽  
Kwang Kyu Kim ◽  
Hyung-Gwan Lee ◽  
Chi-Yong Ahn ◽  
Hee-Mock Oh
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
River Sediment ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Sequence Similarities

A Gram-stain-negative, aerobic, non-motile, rod- and coccus-shaped bacterium, designated strain B6-12T, was isolated from sediment collected from the River Geumho in South Korea. In comparative 16S rRNA gene sequence analysis, the novel strain appeared to be affiliated with the class Alphaproteobacteria and to be most closely related to Kaistia adipata KCTC 12095T, Kaistia dalseonensis DSM 18800T, Kaistia geumhonensis DSM 18799T, Kaistia granuli KCTC 12575T, Kaistia soli KACC 12605T and Kaistia terrae KACC 12910T, with sequence similarities of 96.2–99.1 %. The predominant ubiquinone in the isolate was Q-10, major fatty acids were C18 : 0, C18 : 1ω7c and C19 : 0ω8c cyclo, and genomic DNA G+C content was 63.0 mol%. Based on the phylogenetic and chemotaxonomic evidence and the results of DNA–DNA hybridizations, strain B6-12T represents a novel species in the genus Kaistia , for which the name Kaistia defluvii sp. nov. is proposed. The type strain is B6-12T ( = KCTC 23766T  = JCM 18034T).

Mucilaginibacter mali sp. nov., isolated from rhizosphere soil of apple orchard

2021 ◽  
Author(s):  
Han Sol Kim ◽  
Keun Chul Lee ◽  
Min Kuk Suh ◽  
Ji-Sun Kim ◽  
Kook-Il Han ◽  
...  
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Rhizosphere Soil ◽  
Novel Species ◽  
Apple Orchard ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type

A novel Gram-negative bacterium, designated G2-14T, was isolated from rhizosphere soil sample collected from apple orchard in Chungju-si, Chungcheongbuk-do, Republic of Korea. Strain G2-14T was a strictly aerobic, non-spore-forming, non-motile and short-rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain G2-14T was closely related to Mucilaginibacter myungsuensis HMD1056T (96.9 %) and Mucilaginibacter boryungensis BDR-9T (96.8 %). The major cellular fatty acids (>10 %) of strain G2-14T were summed feature 3 (C16:1 ω6с and/or C16:1 ω7с) and iso-C15:0. The predominant quinone and the major polar lipid were menaquinone-7 and phosphatidylethanolamine, respectively. Strain G2-14T produced acetic acid. The DNA G+C content based on whole genome sequences was 46.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain G2-14T represents a novel species in the genus Mucilaginibacter , for which the name Mucilaginibacter mali sp. nov. is proposed. The type strain is G2-14T (=KCTC 72533T=NBRC 114179T).

Nocardioides marinquilinus sp. nov., isolated from coastal seawater

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2594-2599 ◽  
Author(s):  
Yirang Cho ◽  
Gwang Il Jang ◽  
Byung Cheol Cho
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Coastal Seawater ◽  
Link Type ◽  
The 16S Rrna Gene

A Gram-stain-positive, non-motile, strictly aerobic, non-spore-forming and short rod-shaped bacterial strain, CL-GY44T, was isolated from coastal seawater, Korea. Analysis of the 16S rRNA gene sequence of strain CL-GY44T revealed a clear affiliation with the genus Nocardioides . Based on phylogenetic analysis, strain CL-GY44T showed the closest phylogenetic relationship with Nocardioides ginsengagri BX5-10T and Nocardioides plantarum NCIMB 12834T. Strain CL-GY44T was not able to grow in the presence of NaCl but grew with 0–5.5 % sea salts. The optimum temperature and pH for growth were 30 °C and pH 7.0. The major cellular fatty acids of strain CL-GY44T were C17 : 1ω6c, iso-C16 : 0, C16 : 0 and iso-C15 : 0 and the major menaquinone was MK-8(H4). The cell-wall analysis showed that strain CL-GY44T contained ll-diaminopimelic acid. The genomic DNA G+C content was 71.6 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-GY44T could be clearly distinguished from members of the genus Nocardioides . Thus, strain CL-GY44T should be classified as representing a novel species in the genus Nocardioides , for which the name Nocardioides marinquilinus sp. nov. is proposed. The type strain is CL-GY44T ( = KCCM 90109T = JCM 18459T).

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