scholarly journals Virgibacillus halophilus sp. nov., spore-forming bacteria isolated from soil in Japan

2007 ◽  
Vol 57 (7) ◽  
pp. 1607-1611 ◽  
Author(s):  
Sun-Young An ◽  
Mika Asahara ◽  
Keiichi Goto ◽  
Hiroaki Kasai ◽  
Akira Yokota
Keyword(s):  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Bacterial Strains ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Type Strains ◽  
Spore Forming Bacteria ◽  
Sequence Similarities

Two Gram-positive, round-spore-forming, rod-shaped, halophilic bacterial strains, 5B73CT and 5B133E, were isolated from field soil in Kakegawa, Shizuoka, Japan, and were characterized taxonomically using a polyphasic approach. These two strains were found to comprise strictly aerobic, motile rods that formed subterminal endospores. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains 5B73CT and 5B133E are phylogenetically affiliated to the genus Virgibacillus, exhibiting sequence similarities of 94.1–96.4 % with respect to the type strains of Virgibacillus species. The DNA G+C contents of strains 5B73CT and 5B133E were 42.6 and 42.3 mol%, respectively. The cell-wall peptidoglycan type (meso-diaminopimelic acid), the major cellular fatty acids (anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0) and the quinone type (MK-7) of the isolates support their affiliation to the genus Virgibacillus. On the basis of their genotypic and phenotypic characteristics, the isolates represent a novel species of the genus Virgibacillus, for which the name Virgibacillus halophilus sp. nov. is proposed. The type strain is 5B73CT (=IAM 15308T=KCTC 13935T).

scholarly journals Halobacillus faecis sp. nov., a spore-forming bacterium isolated from a mangrove area on Ishigaki Island, Japan

2007 ◽  
Vol 57 (11) ◽  
pp. 2476-2479 ◽  
Author(s):  
Sun-Young An ◽  
Kaneo Kanoh ◽  
Hiroaki Kasai ◽  
Keiichi Goto ◽  
Akira Yokota
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Mangrove Area ◽  
Ishigaki Island ◽  
Type Strains ◽  
Sequence Similarities

A Gram-positive, spore-forming, rod-shaped halophilic bacterial strain, IGA7-4T, was isolated from a mangrove area on Ishigaki Island (Japan), and was characterized taxonomically using a polyphasic approach. Strain IGA7-4T was strictly aerobic and non-motile and formed central endospores. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain IGA7-4T is affiliated to the genus Halobacillus, and exhibits sequence similarities of 99.6–98.0 % to the type strains of Halobacillus species. Levels of DNA–DNA relatedness between strain IGA7-4T and the type strains of Halobacillus species were 9.5–46.6 %. The DNA G+C content of strain IGA7-4T was 46.5 mol%. The cell-wall peptidoglycan type (Orn–Asp), major cellular fatty acids (anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0) and quinone type (MK-7) of the isolate support its affiliation to the genus Halobacillus. On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data, the isolate represents a novel species of the genus Halobacillus, for which the name Halobacillus faecis sp. nov. is proposed. The type strain is IGA7-4T (=MBIC08268T=IAM 15427T=KCTC 13121T).

scholarly journals Nocardioides terrigena sp. nov., isolated from soil

2007 ◽  
Vol 57 (11) ◽  
pp. 2472-2475 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Soo-Young Lee ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Type Strains

A Gram-positive, rod- or coccoid-shaped bacterial strain, DS-17T, was isolated from a soil in Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain DS-17T grew optimally at around pH 8.0 and 30 °C in the presence of 0.5–1.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-17T belonged to the genus Nocardioides. The chemotaxonomic properties of strain DS-17T were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-2,6-diaminopimelic acid, MK-8(H4) was the predominant menaquinone and iso-C16 : 0, C17 : 1 ω8c and C17 : 0 were the major fatty acids. The DNA G+C content was 71.5 mol%. Strain DS-17T exhibited 16S rRNA gene sequence similarity values of 94.5–96.9 % to the type strains of recognized Nocardioides species. Strain DS-17T could be distinguished from recognized Nocardioides species by differences in phenotypic characteristics. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DS-17T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides terrigena sp. nov. is proposed. The type strain is DS-17T (=KCTC 19217T=JCM 14582T).

scholarly journals Terribacillus saccharophilus gen. nov., sp. nov. and Terribacillus halophilus sp. nov., spore-forming bacteria isolated from field soil in Japan

2007 ◽  
Vol 57 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Sun-Young An ◽  
Mika Asahara ◽  
Keiichi Goto ◽  
Hiroaki Kasai ◽  
Akira Yokota
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Field Soil ◽  
16S Rrna Gene Sequences ◽  
Spore Forming Bacteria

Three strains, 002-048T, RB589 and 002-051T, isolated from field soil in Japan, were characterized using a polyphasic approach. The isolates were Gram-positive, strictly aerobic, non-motile rods that formed ellipsoidal, subterminal endospores. The chemotaxonomic characteristics of these isolates included the presence of meso-diaminopimelic acid as the cell-wall peptidoglycan, anteiso-C15 : 0 and anteiso-C17 : 0 as the major cellular fatty acids and MK-7 as the predominant menaquinone. The DNA G+C content was 44–46 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the isolates represented an independent lineage that is distinct from related taxa and exhibited less than 94.3 % sequence similarity with respect to those taxa. Moreover, a DNA–DNA hybridization analysis showed that the three isolates represented two species. On the basis of their phenotypic and phylogenetic distinctiveness, the isolates represent two species within a novel genus, for which the names Terribacillus saccharophilus gen. nov., sp. nov. and Terribacillus halophilus sp. nov. are proposed. The type strain of T. saccharophilus is 002-048T (=IAM 15309T=KCTC 13936T) and the type strain of T. halophilus is 002-051T (=IAM 15310T=KCTC 13937T).

scholarly journals Sporosarcina saromensis sp. nov., an aerobic endospore-forming bacterium

2007 ◽  
Vol 57 (8) ◽  
pp. 1868-1871 ◽  
Author(s):  
Sun-Young An ◽  
Tomomi Haga ◽  
Hiroaki Kasai ◽  
Keiichi Goto ◽  
Akira Yokota
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
16S Rrna Gene Sequences ◽  
Brackish Lake ◽  
Cellular Fatty Acids ◽  
Type Strains ◽  
Sequence Similarities

Two Gram-positive, endospore-forming, rod-shaped bacterial strains, HG645T and HG711, were respectively isolated from surface water of a brackish lake and sediment of a fishery harbour in Japan and were subsequently characterized taxonomically using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains HG645T and HG711 are affiliated phylogenetically to the genus Sporosarcina, and they exhibit sequence similarities of 95.7–97.3 % to the type strains of Sporosarcina species. DNA–DNA relatedness between strain HG645T and the type strain of the phylogenetically related species Sporosarcina aquimarina was less than 10 %. The DNA G+C content of strains HG645T and HG711 were respectively 46.0 and 45.2 mol%. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cell-wall peptidoglycan type (Lys–Glu), major cellular fatty acids (iso-C15 : 0 and anteiso-C15 : 0) and quinone type (MK-7) of the isolates support their affiliation to the genus Sporosarcina. On the basis of phylogenetic analysis and physiological and chemotaxonomic data, the isolates represent a novel species of the genus Sporosarcina, for which the name Sporosarcina saromensis sp. nov. is proposed. The type strain is strain HG645T (=MBIC08270T=IAM 15429T =KCTC 13119T).

scholarly journals Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea

2005 ◽  
Vol 55 (5) ◽  
pp. 1833-1837 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Soo-Young Lee ◽  
Mi-Hwa Lee ◽  
Tae-Kwang Oh
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
East Sea ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Dna Relatedness ◽  
Type Strains

A Gram-variable, motile, endospore-forming, slightly halophilic bacterial strain, DSW-10T, was isolated from Dokdo, an island located at the edge of the East Sea, Korea, and was characterized taxonomically by using a polyphasic approach. This isolate grew optimally at 37 °C and in the presence of 4–5 % NaCl. Strain DSW-10T had cell-wall peptidoglycan based on meso-diaminopimelic acid, MK-7 as the predominant menaquinone, and anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 as major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified phospholipids. The DNA G+C content was 36·7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DSW-10T is phylogenetically affiliated to the genus Virgibacillus, and exhibited sequence similarity of 95·3–98·7 % to the type strains of Virgibacillus species. DNA–DNA relatedness levels between strain DSW-10T and the type strains of some phylogenetically related Virgibacillus species were in the range 8·4–17·5 %. On the basis of phenotypic properties and phylogenetic and genetic distinctiveness, strain DSW-10T (=KCTC 3933T=DSM 16826T) was classified as the type strain of a novel Virgibacillus species, for which the name Virgibacillus dokdonensis sp. nov. is proposed.

scholarly journals Belliella marina sp. nov., isolated from seawater

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4353-4357 ◽  
Author(s):  
Lei Song ◽  
Hongcan Liu ◽  
Jian Wang ◽  
Ying Huang ◽  
Xin Dai ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Optimum Ph ◽  
The Indian Ocean ◽  
Bacterium Strain

A Gram-stain-negative, rod-shaped bacterium, strain SW112T, was isolated from a seawater sample collected from the Indian Ocean. The strain was strictly aerobic and catalase- and oxidase-positive. Strain SW112T grew at 4–42 °C (optimum 30 °C), at pH 5.5–9.5 (optimum pH 7.5) and in the presence of 0–9.0 % (w/v) NaCl (optimum 2.0–3.0 %). The predominant cellular fatty acids were iso-C15 : 0 (29.7 %), iso-C17 : 03-OH (14.3 %) and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 15.1 %). The major menaquinone was menaquinone-7 and the major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of strain SW112T was 39 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SW112T was related to members of the genus Belliella, showing the highest similarity with Belliella aquatica TS-T86Tand Belliella baltica DSM 15883T (96.5 % and 96.4 % sequence similarity, respectively). On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that strain SW112T represents a novel species of the genus Belliella, for which the name Belliella marina sp. nov. is proposed. The type strain is SW112T ( = CGMCC 1.15180T = KCTC 33694T).

scholarly journals Rheinheimera aestuari sp. nov., a marine bacterium isolated from coastal sediment

2015 ◽  
Vol 65 (Pt_8) ◽  
pp. 2640-2645 ◽  
Author(s):  
Kyunghwa Baek ◽  
Che Ok Jeon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Analyses ◽  
Coastal Sediment ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Family ◽  
Optimum Ph ◽  
Type Strains ◽  
Sequence Similarities

A Gram-stain-negative, strictly aerobic, non-pigmented, motile bacterium with a single polar flagellum, designated H29T, was isolated from coastal sediment of Jeju Island, South Korea. Cells were non-spore-forming rods showing catalase- and oxidase-positive reactions. Growth of strain H29T was observed at 10–40 °C (optimum, 20–25 °C) and pH 6.0–9.0 (optimum, pH 7.0–8.0), and in the presence of 1–4 % (w/v) NaCl (optimum, 2–3 %). Strain H29T contained C16 : 0, iso-C15 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c) as the major fatty acids and ubiquinone-8 (Q-8) as the sole isoprenoid quinone. Phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids. The G+C content of the genomic DNA was 46.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain H29T formed a phyletic lineage with Rheinheimera hassiensis E48T within the genus Rheinheimera of the family Chromatiaceae. Strain H29T was most closely related to Rheinheimera pacifica KMM 1406T, Rheinheimera muenzenbergensis E49T, Rheinheimera hassiensis E48T and Rheinheimera baltica OSBAC1T with 97.8 %, 97.6 %, 97.4 % and 97.2 % 16S rRNA gene sequence similarities, respectively. However, DNA–DNA hybridization values of strain H29T with type strains of these species were lower than 70 %. On the basis of the phenotypic, chemotaxonomic and molecular properties, strain H29T represents a novel species of the genus Rheinheimera, for which the name Rheinheimera aestuarii sp. nov. is proposed. The type strain is H29T ( = KACC 18251T = JCM 30404T).

scholarly journals Deinococcus depolymerans sp. nov., a gamma- and UV-radiation-resistant bacterium, isolated from a naturally radioactive site

2011 ◽  
Vol 61 (6) ◽  
pp. 1448-1453 ◽  
Author(s):  
Dalal Asker ◽  
Tarek S. Awad ◽  
Lynne McLandsborough ◽  
Teruhiko Beppu ◽  
Kenji Ueda
Keyword(s):  
Uv Radiation ◽  
Rrna Gene ◽  
The Novel ◽  
Bacterial Strains ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Radiation Resistant ◽  
Sequence Similarities

Four gamma- and UV-radiation-resistant bacterial strains, designated TDMA-24T, TDMA-24-2, TDMA-24-3 and TDMA-24-4, were isolated from a fresh-water sample collected at Misasa, Tottori, Japan. Cells of these strains were Gram-reaction-positive, non-motile, non-spore-forming, rod-shaped and formed red colonies. The genomic DNA G+C contents ranged from 70.5 to 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolates belong to the genus Deinococcus, the highest sequence similarities being with Deinococcus aquaticus PB314T (98 %) and Deinococcus caeni Ho-08T (97 %). The polar lipid profile of strain TDMA-24T comprised three unidentified phosphoglycolipids, five unidentified glycolipids and seven unidentified polar lipids. MK-8 was the predominant respiratory quinone. Major fatty acids were iso-C15 : 0, C15 : 1ω6c, C15 : 0, C16 : 0 and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). On the basis of their phylogenetic positions and chemotaxonomic and phenotypic characteristics, the novel isolates represent a novel species of the genus Deinococcus, for which the name Deinococcus depolymerans sp. nov. is proposed. The type strain is TDMA-24T ( = JCM 14369T  = NBRC 102115T  = CCUG 53609T).

Planifilum composti sp. nov., a thermophile isolated from compost

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4557-4561 ◽  
Author(s):  
Song-Ih Han ◽  
Jae-Chan Lee ◽  
Hyo-Jin Lee ◽  
Kyung-Sook Whang
Keyword(s):  
Thermophilic Bacteria ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Optimum Ph ◽  
Type Strains ◽  
Sequence Similarities

Two thermophilic bacteria, designated strains P8T and P12, were isolated from compost in Korea. The isolates grew aerobically from 50 to 75 °C (optimum at 55 °C) and at pH 4.0–9.0 (optimum pH 6.5). Aerial mycelia were not observed. Single spores were produced along the substrate hypha. The predominant menaquinone was MK-7. Major fatty acids were iso-C17 : 0, iso-C15 : 0 and iso-C16 : 0. The cell wall contained meso-diaminopimelic acid and the polar lipids were phosphatidylethanolamine, an aminophospholipid and sphingoglycolipid. The DNA G+C contents were 55.9–56.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains P8T and P12 belonged to the genus Planifilum in the family Thermoactinomycetaceae with sequence similarities of 96.1–97.2 %. Levels of DNA–DNA relatedness between strain P8T and the type strains of recognized species of the genus Planifilum ranged from 28.9 to 38.2 %. On the basis of data from the present polyphasic study, strains P8T and P12 are considered to represent a novel species of the genus Planifilum , for which the name Planifilum composti sp. nov. is proposed. The type strain is P8T ( = KACC 16581T = NBRC 108858T).

scholarly journals Nocardioides marinus sp. nov.

2007 ◽  
Vol 57 (4) ◽  
pp. 775-779 ◽  
Author(s):  
Dong Han Choi ◽  
Hye Min Kim ◽  
Jae-Hoon Noh ◽  
Byung Cheol Cho
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Diamino Acid ◽  
Ph Range ◽  
Type Strains

A Gram-positive, slightly halophilic, rod-shaped bacterium, designated CL-DD14T, was isolated from seawater of the East Sea, Korea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CL-DD14T belongs to the genus Nocardioides. Levels of 16S rRNA gene sequence similarity between strain CL-DD14T and the type strains of recognized species of the genus Nocardioides were low (94.1–97.1 %). Strain CL-DD14 T grew over the pH range 6–9 and temperature range 10–40 °C. The strain grew at NaCl concentrations of 0.5–8 % (w/v) with optimum growth at 1–3 % and no growth was observed after 3 weeks on nutrient agar without any salts. It contained ll-diaminopimelic acid as the diamino acid in the cell wall. The major isoprenoid quinone was MK-8(H4) and the major cellular fatty acids were iso-C16 : 0 and 10-methyl C17 : 0. The DNA G+C content was 72.9 mol%. On the basis of phenotypic and phylogenetic data, strain CL-DD14T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides marinus sp. nov. is proposed. The type strain is CL-DD14T (=KCCM 42321T=DSM 18248T).

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