scholarly journals Paenibacillus terrigena sp. nov., isolated from soil

2007 ◽  
Vol 57 (1) ◽  
pp. 70-72 ◽  
Author(s):  
Cheng-Hui Xie ◽  
Akira Yokota
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Reference Species ◽  
Positive Bacterium ◽  
Bacterium Strain

A novel Gram-positive bacterium, strain A35T, was isolated from coastal soil at Chiba, Japan, and was identified as a member of the genus Paenibacillus on the basis of phenotypic and phylogenetic analyses. The bacterium was found to be a facultatively anaerobic and endospore-forming rod. The predominant menaquinone was MK-7, the major cellular fatty acid was anteiso-C15 : 0 and the DNA G+C content was 48.1 mol%. The levels of 16S rRNA gene sequence similarity between strain A35T and Paenibacillus species with validly published names were less than 94 %. Strain A35T was clearly distinguishable from reference species for the genus Paenibacillus. Therefore, on the basis of these data, a novel species of the genus Paenibacillus, Paenibacillus terrigena sp. nov., is proposed. The type strain is A35T (=IAM 15291T=CCTCC AB206026T).

scholarly journals Bacillus seohaeanensis sp. nov., a halotolerant bacterium that contains l-lysine in its cell wall

2006 ◽  
Vol 56 (8) ◽  
pp. 1893-1898 ◽  
Author(s):  
Jae-Chan Lee ◽  
Jee-Min Lim ◽  
Dong-Jin Park ◽  
Che Ok Jeon ◽  
Wen-Jun Li ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
16S Rrna Gene Sequences ◽  
Positive Bacterium

A halotolerant, round-endospore-forming, aerobic, Gram-positive bacterium, designated BH724T, was isolated from a solar saltern at Taean in Korea. Cells of this strain were rod-shaped and found to be non-motile. Strain BH724T grew at salinities of 0–10 % (w/v) NaCl with an optimum of 3 % (w/v) NaCl and at temperatures of 15–50 °C with an optimum of 40 °C. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BH724T belonged to the genus Bacillus and that Bacillus aquimaris TF-12T, Bacillus marisflavi TF-11T and Bacillus vietnamensis JCM 11124T were its closest neighbours, sharing 97.3, 97.2 and 97.0 % 16S rRNA gene sequence similarity, respectively. The genomic DNA G+C content was 39 mol% and the predominant menaquinone was MK-7. Its major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C14 : 0. The peptidoglycan type was A1α, linked directly through l-lysine. On the basis of morphological, chemotaxonomic, physiological and phylogenetic properties, strain BH724T represents a novel species of the genus Bacillus, for which the name Bacillus seohaeanensis sp. nov. is proposed. The type strain is BH724T (=KCTC 3913T=DSM 16464T).

Rhizobium soli sp. nov., isolated from soil

2010 ◽  
Vol 60 (6) ◽  
pp. 1387-1393 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Hwe-Su Yi ◽  
Tae-Kwang Oh ◽  
Choong-Min Ryu
Keyword(s):  
16S Rrna ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Type Strains

A Gram-negative, non-motile, pale-yellow, rod-shaped bacterial strain, DS-42T, was isolated from a soil in Korea and its taxonomic position was investigated by a polyphasic study. Strain DS-42T grew optimally at 25 °C and pH 7.0–8.0. Strain DS-42T did not form nodules on three different legumes, and the nodD and nifH genes were also not detected by PCR. Strain DS-42T contained Q-10 as the predominant ubiquinone. The major cellular fatty acid was C18 : 1 ω7c. The DNA G+C content was 60.8 mol%. Phylogenetic analyses based on 16S rRNA, atpD and recA gene sequences showed that strain DS-42T belonged to the genus Rhizobium. Strain DS-42T showed 16S rRNA gene sequence similarity of 94.1–97.7 % to the type strains of recognized Rhizobium species. DNA–DNA relatedness between strain DS-42T and the type strains of Rhizobium huautlense, R. galegae, R. loessense and R. cellulosilyticum was 13–19 %, indicating that strain DS-42T was distinct from them genetically. Strain DS-42T can also be differentiated from these four phylogenetically related Rhizobium species by various phenotypic properties. On the basis of phenotypic properties, phylogenetic distinctiveness and genetic data, strain DS-42T is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium soli sp. nov. is proposed. The type strain is DS-42T (=KCTC 12873T =JCM 14591T).

scholarly journals Dyadobacter hamtensis sp. nov., from Hamta glacier, located in the Himalayas, India

2005 ◽  
Vol 55 (5) ◽  
pp. 2113-2117 ◽  
Author(s):  
P. Chaturvedi ◽  
G. S. N. Reddy ◽  
S. Shivaji
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Characteristics ◽  
Mountain Ranges ◽  
Himalayan Mountain

Strain HHS 11T was isolated from a water sample collected from the snout of Hamta glacier located in the Himalayan mountain ranges of India. Phenotypic, chemotaxonomic and phylogenetic analyses established the affiliation of the isolate to the genus Dyadobacter. HHS 11T possessed 96 and 95 % 16S rRNA gene sequence similarity with respect to Dyadobacter crusticola and Dyadobacter fermentans, respectively. Furthermore, strain HHS 11T differs from D. crusticola and D. fermentans in a number of phenotypic characteristics. These data suggest that strain HHS 11T represents a novel species of the genus Dyadobacter, for which the name Dyadobacter hamtensis sp. nov. is proposed. The type strain is HHS 11T (=JCM 12919T=MTCC 7023T).

scholarly journals Agromyces arachidissp. nov. Isolated from a Peanut (Arachis hypogaea) Crop Field

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Chandandeep Kaur ◽  
Anil Kumar Pinnaka ◽  
Nitin Kumar Singh ◽  
Monu Bala ◽  
Shanmugam Mayilraj
Keyword(s):  
Arachis Hypogaea ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Crop Field ◽  
Bacterium Strain ◽  
The 16S Rrna Gene

A Gram-positive, yellowish bacterium strain AK-1Twas isolated from soil sample collected from peanut (Arachis hypogaea) crop field and studied by using a polyphasic approach. The organism had morphological and chemotaxonomic properties consistent with its classification in the genusAgromyces. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain AK-1Twas closely related toAgromyces aurantiacus(98.6%) followed byAgromyces soli(98.3%),Agromyces tropicus(97.6%),Agromyces ulmi(97.3%),Agromyces flavus(97.2%), andAgromyces italicus(97.0%), whereas the sequence similarity values with respect to the otherAgromycesspecies with validly published names were between 95.3 and 96.7 %. However, the DNA-DNA hybridization values obtained between strain AK-1Tand other related strains were well below the threshold that is required for the proposal of a novel species. The DNAG+Ccontent of the strain is 71.8 mol%. The above data in combination with the phenotypic distinctiveness of AK-1Tclearly indicate that the strain represents a novel species, for which the nameAgromyces arachidissp. nov. is proposed. The type strain is AK-1T(=MTCC 10524T= JCM 19251T).

scholarly journals Corynebacterium mustelae sp. nov., isolated from a ferret with lethal sepsis

2010 ◽  
Vol 60 (4) ◽  
pp. 871-873 ◽  
Author(s):  
Guido Funke ◽  
Reinhard Frodl ◽  
Kathryn A. Bernard
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Sequence Divergence ◽  
Variable Region ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Lethal Sepsis ◽  
Bacterium Strain ◽  
Sequence Similarity Analysis

A non-lipophilic coryneform bacterium, strain 3105T, was isolated from various tissues of a ferret with lethal sepsis. The strain was characterized by phenotypic and chemotaxonomic methods, which suggested an assignment of the isolate to the genus Corynebacterium. Strain 3105T exhibited the following peculiar features that made it possible to differentiate it phenotypically from all other corynebacteria: its distinctive ‘humid cellar’-like odour, strong adherence to agar and a greenish-beige pigment. Strain 3105T exhibited more than 2.8 % 16S rRNA gene sequence divergence from its closest phylogenetic neighbour, Corynebacterium pseudotuberculosis NCTC 3450T (97.12 % sequence similarity). Analysis of the highly variable region within the rpoB gene sequence showed that strain 3105T exhibited more than 14 % divergence from its closest phylogenetic relative, again C. pseudotuberculosis. Based on the data presented, it is proposed that the ferret isolate should be classified within a novel species, Corynebacterium mustelae sp. nov. (type strain 3105T =CCUG 57279T =DSM 45274T).

scholarly journals Myroides marinus sp. nov., a member of the family Flavobacteriaceae, isolated from seawater

2011 ◽  
Vol 61 (4) ◽  
pp. 938-941 ◽  
Author(s):  
Sung-Heun Cho ◽  
Song-Hee Chae ◽  
Wan-Taek Im ◽  
Seung Bum Kim
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis ◽  
16S Rna ◽  
The Family ◽  
Myroides Odoratimimus ◽  
Bacterium Strain

A Gram-negative, aerobic, non-motile, yellow-pigmented, rod-shaped bacterium (strain JS-08T) isolated from seawater was subjected to a polyphasic taxonomic study. 16S rRNA gene sequence analysis indicated that strain JS-08T belongs to the genus Myroides, a member of the phylum Bacteroidetes. Its closest phylogenetic relative was Myroides odoratimimus JCM 7460T, with which it shared 97.0 % 16S RNA gene sequence similarity. Strain JS-08T contained menaquinone-6 (MK-6) as the predominant menaquinone, and the dominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and a summed feature consisting of iso-C15 : 0 2-OH and/or C16 : 1ω7c. The DNA G+C content of strain JS-08T was 34.2 mol%. Based on phenotypic, genotypic and phylogenetic evidence, it is suggested that strain JS-08T represents a novel species of the genus Myroides, for which the name Myroides marinus sp. nov. is proposed. The type strain is JS-08T ( = KCTC 23023T  = JCM 16529T).

scholarly journals Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment

2004 ◽  
Vol 54 (5) ◽  
pp. 1521-1526 ◽  
Author(s):  
Ma Jesús Montes ◽  
Elena Mercadé ◽  
Núria Bozal ◽  
Jesús Guinea
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Major Cellular Fatty Acid ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis ◽  
The Antarctic

An endospore-forming strain, 20CMT, was isolated from Antarctic sediment and identified as a member of the genus Paenibacillus on the basis of phenotypic and phylogenetic analyses. The organism stained Gram-variable and was facultatively anaerobic. Strain 20CMT was psychrotolerant, growing optimally at 10–15 °C. Like other Paenibacillus species, it contained anteiso-C15 : 0 as the major cellular fatty acid. The DNA G+C content was 40·7 mol%. 16S rRNA gene sequence analysis placed strain 20CMT within the Paenibacillus cluster, with a similarity value of 99·5 % to Paenibacillus macquariensis DSM 2T. DNA–DNA hybridization experiments between the Antarctic isolate and P. macquariensis DSM 2T revealed a reassociation value of 47 %, indicating that strain 20CMT and P. macquariensis DSM 2T belong to different species. Based on evaluation of morphological, physiological, chemotaxonomic and phylogenetic analyses, a novel species, Paenibacillus antarcticus sp. nov., is proposed; the type strain is 20CMT (=LMG 22078T=CECT 5836T).

scholarly journals Pediococcus ethanolidurans sp. nov., isolated from the walls of a distilled-spirit-fermenting cellar

2006 ◽  
Vol 56 (10) ◽  
pp. 2405-2408 ◽  
Author(s):  
Limeng Liu ◽  
Bin Zhang ◽  
Huichun Tong ◽  
Xiuzhu Dong
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Hebei Province ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Glucose Fermentation ◽  
The Mean

Two novel Pediococcus strains, Z-9T and Z-17, were isolated from a distilled-spirit-fermenting cellar in Hebei Province, China. The cells were Gram-positive, non-spore-forming, non-motile cocci and occurred in pairs or tetrads. The strains were facultatively anaerobic and produced only lactic acid [d(−) and l(+) isomers in the ratio 50 : 50] from glucose fermentation. Catalase activity was not present. Both strains were able to grow in 6.5 % ethanol and at pH 3.5, but not in 4 % NaCl. The mean genomic G+C content of the two strains was 39.5±0.5 mol% (39 mol% for the type strain, Z-9T). The levels of 16S rRNA gene sequence similarity between the two novel strains and related species of the genus Pediococcus ranged from 98.3 to 98.7 %. The levels of DNA–DNA relatedness between strain Z-9T and the phylogenetically closely related pediococci Pediococcus damnosus LMG 11484T, Pediococcus inopinatus LMG 11409T, Pediococcus parvulus LMG 11486T and Pediococcus cellicola LMG 22956T were 14.6, 33, 28.7 and 16.8 %, respectively. On the basis of phenotypic, genotypic and phylogenetic analyses, a novel species, Pediococcus ethanolidurans sp. nov., is proposed, with strain Z-9T (=AS 1.3889T=LMG 23354T) as the type strain.

scholarly journals Pedobacter roseus sp. nov., isolated from a hypertrophic pond, and emended description of the genus Pedobacter

2006 ◽  
Vol 56 (8) ◽  
pp. 1831-1836 ◽  
Author(s):  
Chung Yeon Hwang ◽  
Dong Han Choi ◽  
Byung Cheol Cho
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
The Family ◽  
Emended Description ◽  
National University ◽  
Seoul National University

A Gram-negative, pink-coloured, rod-shaped, non-flagellated bacterium, designated CL-GP80T, was isolated from a hypertrophic pond located within the campus of Seoul National University, Korea. Analysis of its 16S rRNA gene sequence revealed that strain CL-GP80T belongs to the family Sphingobacteriaceae and is closely related to Pedobacter heparinus ATCC 13125T (95.8 % sequence similarity) and to other members of the genus Pedobacter (90.8–95.3 % similarity). Temperature and pH ranges for growth were 5–33 °C and pH 6–8, respectively. The DNA G+C content was 41.3 mol%. The major fatty acids were iso-C15 : 0 (37.0 %), iso-C15 : 0 2-OH and/or C16 : 1 ω7c (24.5 %), and iso-C17 : 0 3-OH (11.3 %). Phenotypic, chemotaxonomic and phylogenetic analyses indicated that strain CL-GP80T could be assigned to the genus Pedobacter, but distinguished from recognized species of the genus. Strain CL-GP80T (=KCCM 42272T=JCM 13399T) is therefore proposed as the type strain of a novel species, for which the name Pedobacter roseus sp. nov. is proposed.

scholarly journals Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant

2005 ◽  
Vol 55 (5) ◽  
pp. 2149-2153 ◽  
Author(s):  
Hanli de Beer ◽  
Celia J. Hugo ◽  
Piet J. Jooste ◽  
Anne Willems ◽  
Marc Vancanneyt ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Processing Plant ◽  
Rrna Gene Sequence ◽  
Reference Species ◽  
Taxonomic Approach ◽  
Gram Negative Organisms

Yellow-pigmented, Gram-negative organisms isolated from raw chicken were investigated by means of a polyphasic taxonomic approach and were shown to represent a novel species in the genus Chryseobacterium, for which the name Chryseobacterium vrystaatense sp. nov. is proposed. Its nearest phylogenetic neighbours were Chryseobacterium joostei, Chryseobacterium indologenes and Chryseobacterium gleum, which showed 16S rRNA gene sequence similarity levels of 96·9, 97·1 and 96·1 %, respectively. Levels of DNA–DNA hybridization between strains of C. vrystaatense and Chryseobacterium reference species were below 46 %. Strain LMG 22846T (=CCUG 50970T) was chosen as the type strain and has a DNA G+C content of 37·1 mol%.

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