scholarly journals Gongronella eborensis sp. nov., from vineyard soil of Alentejo (Portugal)

2020 ◽  
Vol 70 (5) ◽  
pp. 3475-3482 ◽  
Author(s):  
Maria Rosário Martins ◽  
Carla Santos ◽  
Célia Soares ◽  
Cledir Santos ◽  
Nelson Lima
Keyword(s):  
Ribosomal Dna ◽  
Sequence Data ◽  
Novel Species ◽  
Molecular Data ◽  
Fungal Species ◽  
Proteomic Profiling ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Vineyard Soil ◽  
Tof Ms

This study describes a novel fungal species belonging to the genus Gongronella. During a previous work focusing on metalaxyl degradation by Mucorales strains, two isolates from vineyard soil samples collected in the Alentejo region, south Portugal, were identified as a putative novel species based on combined molecular and MALDI-TOF MS data. This new species is described here using a polyphasic approach that combines morphology, internal transcribed spacer of ribosomal DNA (ITS) and 28S ribosomal DNA (LSU) sequence data analysis and proteomic profiling by MALDI-TOF MS. Phenotypic and molecular data enabled this novel species to be clearly distinguished from other Gongronella species with results of combined ITS+LSU analysis showing that the Gongronella species is related to Gongronella butleri and Gongronella brasiliensis. Therefore, from the results of morphological and molecular analyses, isolates MUM 10.262 and MUM 10.263 seem to represent a new Gongronella species and the name Gongronella eborensis sp. nov. is proposed, with the ex-type strain MUM 10.262 (=CCMI 1100=CBS 128763).

Identification of hypocrealean reptile pathogenic isolates with MALDI-TOF MS

2018 ◽  
Vol 57 (6) ◽  
pp. 694-702
Author(s):  
Juliane Schneider ◽  
Tilo Heydel ◽  
Michael Pees ◽  
Wieland Schrödl ◽  
Volker Schmidt
Keyword(s):  
Ribosomal Dna ◽  
Fungal Pathogens ◽  
Fungal Infections ◽  
Large Subunit ◽  
Fungal Keratitis ◽  
Internal Transcribed Spacer Region ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Purpureocillium Lilacinum ◽  
Tof Ms

Abstract Biotyper analysis of Nannizziopsis guarroi, a fatal fungal pathogen in lizards, was described recently. Hypocrealean fungal infections in captive reptiles appear with an increasing frequency during the last decade. Therefore, the aim of this study was to proof Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as diagnostic tool for the identification of reptile pathogenic hypocrealean fungi. Ten fungal isolates obtained from nine reptiles with fungal glossitis, disseminated visceral mycosis, pneumomycosis, and fungal keratitis were analyzed. Phylogeny consisted of fragments of the large subunit of nuclear encoded ribosomal DNA (D1/D2, LSU) and the internal transcribed spacer region 1 of nuclear encoded ribosomal DNA (ITS1) as well as the protein coding gene translation elongation factor 1 alpha (TEF). Results revealed unanimously two Metarhizium granulomatis genotypes in a total of three isolates, various M. viride genotypes (n = 3), two different Purpureocillium lilacinum isolates as well as one isolate of each P. lavendulum and Beauveria bassiana. Purpureocillium lilacinum and B. bassiana are likewise frequently employed as a mycoinsecticide and mycoacaricide in agriculture on a worldwide scale and have occasionally been reported in man, causing fungal keratitis, sclerokeratitis, nosocomial infections in immunosuppressed patients, as well as cavitary pulmonary disease and cutaneous hyalohyphomycosis in immunocompetent patients. According to the results establishment of Biotyper analysis for faster differentiation of reptile-associated fungal pathogens is entirely justified.

Use of MALDI-TOF MS for fungal species distribution of interdigital intertrigo in seafarers, Dakar, Senegal

2020 ◽  
Vol 30 (3) ◽  
pp. 100974
Author(s):  
K. Diongue ◽  
D. Samb ◽  
M.C. Seck ◽  
M.A. Diallo ◽  
M. Ndiaye ◽  
...  
Keyword(s):  
Species Distribution ◽  
Fungal Species ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Matthew Sorensen ◽  
Courtney E. Chandler ◽  
Francesca M. Gardner ◽  
Salma Ramadan ◽  
Prasanna D. Khot ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Lipid Extraction ◽  
Fungal Species ◽  
Steel Matrix ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
Lipid Biomarker ◽  
Biomarker Analysis ◽  
Tof Ms

AbstractRapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker analysis will therefore improve patient outcomes and antimicrobial stewardship. However, current lipid extraction methods require significant hands-on time and are thus not suited for direct adoption as a clinical assay for microbial identification. Here, we have developed a method for lipid extraction directly on the surface of stainless-steel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plates, termed fast lipid analysis technique or FLAT, which facilitates the identification of bacterial and fungal species using a sub-60-minute workflow. Additionally, our method detects lipid A modifications in Gram-negative bacteria that are associated with antimicrobial resistance, including to colistin.

“MALDI-TOF-MS serum proteomic profiling in dairy cows reared in POP-contaminated areas”

2010 ◽  
Vol 150 ◽  
pp. 131-131 ◽  
Author(s):  
Davide Bertarelli ◽  
Giulia Gardini ◽  
Flavia Girolami ◽  
Monica Carletti ◽  
Andrea Urbani ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Proteomic Profiling ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Leptospira ainlahdjerensis sp. nov., Leptospira ainazelensis sp. nov., Leptospira abararensis sp. nov. and Leptospira chreensis sp. nov., four new species isolated from water sources in Algeria

2021 ◽  
Vol 71 (12) ◽  
Author(s):  
Anissa Amara Korba ◽  
Hakim Lounici ◽  
Malia Kainiu ◽  
Antony T. Vincent ◽  
Jean-François Mariet ◽  
...  
Keyword(s):  
Novel Species ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Maldi Tof Ms ◽  
Link Type ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Leptospira strains were isolated from freshwater sampled at four sites in Algeria and characterized by whole-genome sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The cells were spiral-shaped and motile. Phylogenetic and MALDI-TOF MS analyses showed that the strains can be clearly distinguished from the other described species in the genus Leptospir a, therefore representing two novel species of the pathogen subclade P1 and two novel species of the saprophyte subclade S1. The names Leptospira ainlahdjerensis sp. nov. (type strain 201903070T=KIT0297T=CIP111912T), Leptospira ainazelensis sp. nov. (201903071T=KIT0298T=CIP111913T), Leptospira abararensis sp. nov. (201903074T=KIT0299T=CIP111914T) and Leptospira chreensis (201903075T=KIT0300T=CIP111915T) are proposed.

Proteomic profiling of occupational medicamentosa-like dermatitis induced by trichloroethylene in serum based on MALDI-TOF MS

2014 ◽  
Vol 15 (4) ◽  
pp. 519-526 ◽  
Author(s):  
Wei Liu ◽  
Wen-Xu Hong ◽  
Yanfang Zhang ◽  
Peiwu Huang ◽  
Xifei Yang ◽  
...  
Keyword(s):  
Proteomic Profiling ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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