Brevibacillus marinus sp. nov., a thermophilic bacterium isolated from deep sea sediment in the South China Sea

2021 ◽  
Vol 71 (12) ◽  
Author(s):  
Ke-Xin Wang ◽  
Cun Li ◽  
Yuan-Qiu He ◽  
Lin-Qing Cui ◽  
Rou-Wen Chen ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Thermophilic Bacterium ◽  
The South China Sea ◽  
Diaminopimelic Acid ◽  
The South ◽  
Circular Chromosome ◽  
Content Type ◽  
China Sea ◽  
Link Type

A novel thermophilic bacterium, designated SCSIO 07484T, was isolated from marine sediment sampled in the South China Sea. Growth occurred at 30–60 °C, pH 6.0–8.0 and in the presence of 0–3 % (w/v) NaCl. Cells of strain SCSIO 07484T were rod-shaped and flagellum-forming. No soluble pigment was observed. The phylogenetic analysis of the 16S rRNA gene sequences indicated that SCSIO 07484T belonged to the family Paenibacillaceae and clustered with members of the genus Brevibacillus in the phylogenetic trees with less than 96.2 % similarities. The cell wall contained meso-diaminopimelic acid. Whole-cell hydrolysates contained arabinose, glucose and ribose. The predominant menaquinone was MK-7. Major fatty acids were iso-C16 : 0, iso-C15 : 0, C16 : 0 and iso-C14 : 0. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine were its diagnostic polar lipids. The whole genome size of strain SCSIO 07484T was 4 079 826 bp with a DNA G+C content of 56.2 mol%, including one circular chromosome of 3 978392 bp and one plasmid of 101434 bp. Based on the polyphasic analysis of strain SCSIO 07484T, it is considered to represent a novel species of the genus Brevibacillus , for which the name Brevibacillus marinus sp. nov. is proposed with the type strain SCSIO 07484T (=DSM 106769T=CGMCC 1.15814T).

scholarly journals Actinomadura craniellae sp. nov., isolated from a marine sponge in the South China Sea

2019 ◽  
Vol 69 (4) ◽  
pp. 1207-1212 ◽  
Author(s):  
Lei Li ◽  
Qi-hang Xu ◽  
Xiao-tian Wang ◽  
Hou-wen Lin ◽  
Yan-hua Lu
Keyword(s):  
South China Sea ◽  
South China ◽  
Marine Sponge ◽  
Type Species ◽  
The South China Sea ◽  
Diaminopimelic Acid ◽  
The South ◽  
Content Type ◽  
China Sea ◽  
Link Type

A novel marine actinomycete, designated LHW63021T, was isolated from a marine sponge, genus Craniella, collected in the South China Sea. A polyphasic approach was applied to characterize the taxonomic position of this strain. The strain was found to have scarce aerial mycelia that differentiated into spore chains. The cell-wall hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid. Glucose, galactose, mannose and madurose were found in the whole-cell hydrolysates. The dominant polar lipids were phosphatidylinositol and diphosphatidylglycerol. MK-9(H6) and MK-9(H8) were the predominant menaquinones. The major fatty acids were iso-C16 : 0, iso-C18 : 0, 10-methyl C17 : 0 and C18 : 1 ω9c. The DNA G+C content based on the draft genome sequence was 72.0 mol%. 16S rRNA gene sequence analysis indicated that strain LHW63021T was a member of the genus Actinomadura and had the highest similarity to Actinomadura echinospora DSM 43163T (97.3 %). Phylogenetic trees supported their close relationship. The average nucleotide identity and digital DNA–DNA hybridization values between the whole genome sequences of strain LHW63021T and A. echinospora DSM 43163T were 79.13 and 23.20 %, respectively. The evidence from the polyphasic study shows that strain LHW63021T represents a novel species of the genus Actinomadura , for which the name Actinomadura craniellae sp. nov. is proposed. The type strain is LHW63021T (=DSM 106125T=CCTCC AA 2018015T).

scholarly journals Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1712-1717 ◽  
Author(s):  
Daochen Zhu ◽  
Shoko-Hosoi Tanabe ◽  
Changxiao Xie ◽  
Daiske Honda ◽  
Jianzhong Sun ◽  
...  
Keyword(s):  
South China Sea ◽  
16S Rrna ◽  
South China ◽  
Type Species ◽  
The South China Sea ◽  
Diaminopimelic Acid ◽  
The South ◽  
Content Type ◽  
China Sea ◽  
Link Type

An alkaliphilic and halotolerant Gram-stain-positive bacterium, which was isolated from sediment samples from the South China Sea, was subjected to a taxonomic study. The isolate, strain L1T, grew well at a wide range of temperatures and pH values, 10.0–45.0 °C and pH 6–11, with optima at 30 °C and pH 9.0, respectively. The growth of strain L1T occurred at total salt concentrations of 0–10 % (w/v) with an optimum at 2 % (w/v). Phylogenetic analysis based on 16S rRNA sequence comparison indicated that the isolate represented a member of the genus Bacillus . The strains most closely related to strain L1T were Bacillus nanhaiisediminis JCM 16507T, Bacillus halodurans DSM 497T and Bacillus pseudofirmus DSM 8715T, with 16S rRNA similarities of 96.5 %, 95.9 % and 95.7 %, respectively. DNA–DNA hybridization of strain L1T with the type strains of the most closely related species, B. nanhaiisediminis JCM 16507T, B. halodurans DSM 497T and B. pseudofirmus DSM 8715T, showed reassociation values of about 21.7 %, 14.3 % and 13.9 %, respectively. The DNA G+C content of strain L1 T was 40.76 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids of strain L1T were iso-C14 : 0 and anteiso-C15 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the phenotypic and phylogenetic characteristics, it is proposed that strain L1T ( = JCM 18543T = DSM 26145T) should be classified as the type strain of Bacillus ligniniphilus sp. nov.

scholarly journals Puteibacter caeruleilacunae gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from Yongle Blue Hole in the South China Sea

2020 ◽  
Vol 70 (3) ◽  
pp. 1623-1629 ◽  
Author(s):  
Wen Sun ◽  
Tianyu Fu ◽  
Chao Jia ◽  
Liang Fu ◽  
Shun Zhou ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
The South China Sea ◽  
The South ◽  
Content Type ◽  
China Sea ◽  
Link Type ◽  
Facultatively Anaerobic ◽  
Blue Hole ◽  
The Family

Blue holes are unique geomorphological units characterized by steep redox and biogeochemical gradients. Yongle Blue Hole is located on the largest atoll (Yongle Atoll) of the western Xisha Islands in the South China Sea. A Gram-stain-negative, facultatively anaerobic, non-motile, non-flagellated marine bacterium with creamy white colonies, designated JC036T, was isolated from Yongle Blue Hole. Cells were short-rod-shaped and catalase-negative. 16S rRNA gene sequence analysis showed that sequence similarities were lower than 91.6 % against all validly named species in the family Prolixibacteraceae ; a reconstructed phylogenetic tree indicated that strain JC036T formed a lineage with strains in the family Prolixibacteraceae . Growth occurred at 4–37 °C (optimum, 28 °C), at pH 5.0–9.0 (optimum, 7.0) and in the presence of 2–6 % (w/v) NaCl (optimum, 3 %). The prevalent isoprenoid quinone of strain JC036T was menaquinone-7 (MK-7). Iso-C15 : 0 and iso-C17 : 0 3-OH were the predominant fatty acids. The major polar lipids included a phospholipid, phosphatidylethanolamine, an aminophospholipid and four unidentified lipids. The genomic DNA G+C content of strain JC036T was 37.8 mol%. Based on physiological and biochemical characteristics and whole genome comparisons, we propose a new genus and species, Puteibacter caeruleilacunae gen. nov., sp. nov., within the family Prolixibacteraceae . The type strain of Puteibacter caeruleilacunae is JC036T (=JCM 33128T=MCCC 1K03579T). From this study, a deeper understanding of the community of the microorganism and their roles in biogeochemical cycles, especially anaerobic bacteria, is provided.

scholarly journals Nitrincola iocasae sp. nov., a bacterium isolated from sediment collected at a cold seep field in the South China Sea

2020 ◽  
Vol 70 (9) ◽  
pp. 4897-4902 ◽  
Author(s):  
Huan Zhang ◽  
Hao Wang ◽  
Hao Chen ◽  
Qinglei Sun ◽  
Zhaoshan Zhong ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Type Species ◽  
The South China Sea ◽  
Cold Seep ◽  
The South ◽  
Content Type ◽  
China Sea ◽  
Link Type ◽  
A Genome

A novel bacterium, designated strain KXZD1103T, was isolated from sediment collected at a cold seep field of the Formosa Ridge in the South China Sea. Cells were Gram-stain-negative, facultatively anaerobic, motile, oxidase- and catalase-positive, and grew optimally at 28 °C, pH 6.0–pH 7.0 and in the presence of 1–3 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (C18 : 1  ω7c/C18 : 1  ω6c), summed feature 3 (C16 : 1  ω7c/C16 : 1  ω6c) and C16 : 0. The major respiratory ubiquinone was Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Analysis of 16S rRNA gene sequences revealed that strain KXZD1103T grouped with members of the genus Nitrincola , with Nitrincola lacisaponensis 4CAT (98.1 % sequence similarity) and Nitrincola schmidtii R4-8T (97.7 %) as its closest neighbours. Genome sequencing revealed a genome size of 4.17 Mb and a DNA G+C content of 50.1 %. Genomic average nucleotide identity values for strain KXZD1103T with the type strains within the genus Nitrincola ranged from 71.0 to 75.7 %, while the in silico DNA–DNA hybridization values for strain KXZD1103T with these strains ranged from 16.1 to 21.6 %. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, strain KXZD1103T is considered to represent a novel species of the genus Nitrincola , for which the name Nitrincola iocasae sp. nov. is proposed. The type strain is KXZD1103T (=KCTC 72678T=MCCC 1K04283T).

scholarly journals Devosia marina sp. nov., isolated from deep seawater of the South China Sea, and reclassification of Devosia subaequoris as a later heterotypic synonym of Devosia soli

2020 ◽  
Vol 70 (5) ◽  
pp. 3062-3068 ◽  
Author(s):  
Yang Liu ◽  
Juan Du ◽  
Jun Zhang ◽  
Qiliang Lai ◽  
Zongze Shao ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
South China Sea ◽  
South China ◽  
Type Species ◽  
The South China Sea ◽  
The South ◽  
Deep Seawater ◽  
Content Type ◽  
China Sea ◽  
Link Type

A Gram-stain-negative, aerobic, rod-shaped and motile bacterial strain, designated L53-10-65T, was isolated from deep seawater of the South China Sea. Strain L53-10-65T was found to grow at 4–41 °C (optimum, 28 °C), at pH 5.0–9.0 (pH 7.0–8.0) and in 0–7 % (w/v) NaCl (2 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain L53-10-65T fell within the genus Devosia , with the highest similarity to Devosia indica IO390501T (98.4 %), followed by ‘ Devosia lucknowensis ’ L15T (97.7 %) and Devosia riboflavina IFO 13584T (96.8 %). The digital DNA–DNA hybridization (dDDH) values between strain L53-10-65T and the three relatives above were 43.7, 21.5 and 20.9 %, respectively; the average nucleotide identity (ANI) values were 91.3, 78.4 and 76.8 %, respectively. These values were below the 70 % dDDH and 95–96 % ANI thresholds for bacterial species delineation. The major cellular fatty acids of strain L53-10-65T were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), C18 : 1 ω7c 11-methyl and C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The respiratory quinone was identified as Q-10. The DNA G+C content of strain L53-10-65T was 61.3 mol%. Based on the results of phylogenetic analysis and distinctive phenotypic characteristics, strain L53-10-65T is concluded to represent a novel species of the genus Devosia , for which the name Devosia marina sp. nov. is proposed. The type strain of the species is L53-10-65T (=MCCC 1A05139T=KCTC 72888T). Moreover, we propose that Devosia subaequoris is a later heterotypic synonym of Devosia soli based on the present results.

US-China sea disputes will draw in allies

2015 ◽  
Keyword(s):  
South China Sea ◽  
South China ◽  
Land Reclamation ◽  
The South China Sea ◽  
The South ◽  
China Policy ◽  
Content Type ◽  
China Sea ◽  
Us Military ◽  
Military Engagement

Significance Increased anxiety over Chinese land reclamation in the South China Sea, particularly its construction of an airstrip and harbour on Fiery Cross Reef, is drawing considerable attention to US policy in the region where US allies have been pressing for greater involvement. Impacts Regional and US rhetoric on Chinese reclamation may provoke an increased demonstration of US military engagement in the region. However, the size and scale of these activities will be highly dependent on the pace and character of Chinese activities. This issue is likely to be featured conspicuously by Carter at the Shangri-la Dialogue in Singapore. It will almost certainly raise difficulties during Chinese President Xi Jinping's September visit to Washington. Given Hillary Clinton's previous statements, pursuing a 'tough' China policy may become a prominent theme of her presidential bid.

Nuclear weapons complicate South China Sea disputes

2016 ◽  
Keyword(s):  
United States ◽  
South China Sea ◽  
South China ◽  
Hainan Island ◽  
The United States ◽  
The Philippines ◽  
The South China Sea ◽  
The South ◽  
Content Type ◽  
China Sea

Significance However, China's navy already has an operational sea-based nuclear deterrent based on Hainan Island. The deployment of nuclear-armed submarines, and their need to reach the mid-Pacific to threaten the continental United States, makes the South China Sea an arena not just of maritime disputes but of US-China military rivalry. Impacts The strategic importance of the Philippines, Taiwan and Singapore to the United States will increase. A new defence agreement with the Philippines will, as of last month, support US military activities in the area. Washington will encourage greater Japanese involvement in the South China Sea; as long as Shinzo Abe is prime minister, Japan will oblige.

Japan-Malaysia defence ties may estrange Beijing

2015 ◽  
Keyword(s):  
South China Sea ◽  
South China ◽  
Prime Minister ◽  
Armed Forces ◽  
Coast Guard ◽  
The South China Sea ◽  
Strategic Partnership ◽  
The South ◽  
Content Type ◽  
China Sea

Subject The outlook for deepening defence links between Japan and Malaysia. Significance Chinese Politburo member Yu Zhengsheng warned visiting Japanese lawmakers on June 29 that the South China Sea maritime disputes have "nothing to do with Japan". This comes amid new developments in South-east Asian governments' efforts to strengthen security cooperation with Japan. Among them is the agreement between Malaysian Prime Minister Najib Razak and Japanese Prime Minister Shinzo Abe on May 25 to elevate bilateral ties to the level of 'Strategic Partnership'. This reflects Malaysia's growing concern over China's actions in the South China Sea, which are increasingly expanding southwards, affecting Malaysian-claimed territorial waters. Malaysian officials on June 9 announced a complaint to China over a Chinese coast guard vessel near Luconia Shoals. Impacts The Philippines's armed forces are less powerful than Malaysia's, implying more scope to develop Japan-Philippines defence ties. Vietnam, and perhaps Indonesia, may be the next ASEAN countries to seek deeper defence ties with Japan. Malaysian criticism of China over the South China Sea could see a more assertive ASEAN over disputed maritime claims.

US tensions drive China closer to ASEAN

2018 ◽  
Keyword(s):  
South China Sea ◽  
South China ◽  
Code Of Conduct ◽  
The South China Sea ◽  
Maritime Security ◽  
Security Cooperation ◽  
The South ◽  
Content Type ◽  
China Sea

Subject ASEAN-China security cooperation. Significance China-ASEAN security cooperation has moderated the assertiveness Beijing displayed in the South China Sea during the first half of this year. In October, China and the ASEAN states held their first ever joint naval exercise. Impacts The verbal statements on regional maritime security cooperation will increasingly be followed by concrete actions. Negotiations over a Code of Conduct for the South China Sea will advance slowly and contentiously. China-Philippines joint energy explorations in the South China Sea will remain hamstrung by Philippine constitutional conditions.

China-US rivalry in South China Sea is muted for now

2018 ◽  
Keyword(s):  
South China Sea ◽  
South China ◽  
The United States ◽  
The Philippines ◽  
The South China Sea ◽  
South East Asia ◽  
Asian Countries ◽  
The South ◽  
Content Type ◽  
China Sea

Subject The South China Sea dispute. Significance China and the United States increased their military activities in the South China Sea in January and February, with US ‘freedom of navigation operations’ (FONOPs) pushing back on Chinese maritime jurisdictional claims in the area. The Philippines before June 2016 contested China’s expansive claims. Increased rivalry between Beijing and Washington in South-east Asia raises the risk of a dangerous naval confrontation. Impacts The Philippines will continue to solicit investment from China. China is unlikely to undertake actions in the South China Sea that would seriously irk the Philippines. South-east Asian countries will emphasise the importance of the region not becoming a theatre for China-US rivalry.

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