scholarly journals Recurrent candidaemia in a neonate with Hirschsprung's disease: fluconazole resistance and genetic relatedness of eight Candida tropicalis isolates

2006 ◽  
Vol 55 (4) ◽  
pp. 423-428 ◽  
Author(s):  
Pei Pei Chong ◽  
David Ching-Soo Chieng ◽  
Lee Yean Low ◽  
Asma Hafeez ◽  
Mariana Nor Shamsudin ◽  
...  
Keyword(s):  
Candida Tropicalis ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Genetic Relatedness ◽  
Infectious Agent ◽  
Venous Catheter ◽  
Drug Susceptibility ◽  
Fluconazole Resistant ◽  
Susceptibility Tests

The incidence of candidaemia among immunocompromised patients in Malaysia is increasing at an alarming rate. Isolation of clinical strains that are resistant to fluconazole has also risen markedly. We report here the repeated isolation of Candida tropicalis from the blood of a neonatal patient with Hirschsprung's disease. In vitro fluconazole susceptibility tests of the eight isolates obtained at different time points showed that seven of the isolates were resistant and one isolate was scored as susceptible dose-dependent. Random amplification of polymorphic DNA fingerprinting of the isolates using three primers and subsequent phylogenetic analysis revealed that these isolates were highly similar strains having minor genetic divergence, with a mean pairwise similarity coefficient of 0·893±0·041. The source of the infectious agent was thought to be the central venous catheter, as culture of its tip produced fluconazole-resistant C. tropicalis. This study demonstrates the utility of applying molecular epidemiology techniques to complement traditional mycological culture and drug susceptibility tests for accurate and appropriate management of recurrent candidaemia and highlights the need for newer antifungals that can combat the emergence of fluconazole-resistant C. tropicalis strains.

scholarly journals Synergistic Activity of Chloroquine with Fluconazole against Fluconazole-Resistant Isolates of Candida Species

2014 ◽  
Vol 59 (2) ◽  
pp. 1365-1369 ◽  
Author(s):  
Yali Li ◽  
Zhe Wan ◽  
Wei Liu ◽  
Ruoyu Li
Keyword(s):  
Candida Albicans ◽  
Synergistic Effect ◽  
Candida Tropicalis ◽  
Candida Krusei ◽  
Synergistic Activity ◽  
Broth Microdilution ◽  
Content Type ◽  
Fluconazole Resistant ◽  
Susceptibility Tests

ABSTRACTThein vitroactivity of chloroquine and the interactions of chloroquine combined with fluconazole against 37Candidaisolates were tested using the broth microdilution, disk diffusion, and Etest susceptibility tests. Synergistic effect was detected with 6 of 9 fluconazole-resistantCandida albicansisolates, withCandida kruseiATCC 6258, and with all 12 fluconazole-resistantCandida tropicalisisolates.

scholarly journals In Vitro Susceptibility of Mycobacterium abscessus and Mycobacterium fortuitum Isolates to 30 Antibiotics

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Yaojie Shen ◽  
Xuyang Wang ◽  
Jialin Jin ◽  
Jing Wu ◽  
Xuelian Zhang ◽  
...  
Keyword(s):  
Public Health Problem ◽  
Drug Susceptibility ◽  
Mycobacterium Abscessus ◽  
Mycobacterium Fortuitum ◽  
In Vitro Susceptibility ◽  
Antitubercular Drugs ◽  
Microdilution Method ◽  
Susceptibility Tests ◽  
Susceptibility Profiles

Objective. Nontuberculous mycobacteria (NTM) cause various diseases in humans and animals. Recently, the prevalence of NTM-related disease has been on the rise, becoming an emerging public health problem. The aim of this study was to determine the antibiotic susceptibility profiles of clinical isolates of Mycobacterium abscessus and Mycobacterium fortuitum. Methods. We performed susceptibility tests on 37 clinical NTM isolates to 30 antibiotics with the microdilution method recommended by the Clinical and Laboratory Standards Institute. Results. Both M. abscessus and M. fortuitum were highly resistant to antitubercular drugs such as isoniazid, rifampin, ethambutol, clofazimine, ethionamide, and rifabutin. M. abscessus showed the lowest resistant rates to cefoxitin (10%), azithromycin (10%), amikacin (10%), and clarithromycin (20%) and very high resistant to sulfamethoxazole, vancomycin, oxacillin, clindamycin, and all fluoroquinolones. M. fortuitum showed low resistance to tigecycline (0%), tetracycline (0%), cefmetazole (12%), imipenem (12%), linezolid (18%), and the aminoglycosides amikacin (0%), tobramycin (0%), neomycin (0%), and gentamycin (24%). Conclusion. Amikacin, cefoxitin, and azithromycin have the highest in vitro activity against M. abscessus. Isolates of M. fortuitum need to be individually evaluated for drug susceptibility before choosing an effective antimicrobial regimen for treatment of infections.

Hirschsprung's disease: A comparison of the nervous control of ganglionic and aganglionic smooth muscle in vitro

1987 ◽  
Vol 22 (5) ◽  
pp. 431-435 ◽  
Author(s):  
Lars Torsten Larsson ◽  
Gerhard Malmfors ◽  
Claes Wahlestedt ◽  
Stefan Leander ◽  
Rolf Håkanson
Keyword(s):  
Smooth Muscle ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Nervous Control

scholarly journals Disseminated cryptococcosis and fluconazole resistant oral candidiasis in a patient with acquired immunodeficiency syndrome (AIDS)

2010 ◽  
Vol 4 (10) ◽  
pp. 674-678 ◽  
Author(s):  
Rajendra J Kothavade ◽  
Chetan M Oberai ◽  
Arvind G Valand ◽  
Mehroo H Panthaki
Keyword(s):  
Oral Candidiasis ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Body Parts ◽  
Root Cause ◽  
Fluconazole Resistant ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome ◽  
In Vitro Drug Susceptibility

Disseminated cryptococcosis and recurrent oral candidiasis was presented in a-heterosexual AIDS patient. Candida tropicalis (C.tropicalis) was isolated from the oral pseudomembranous plaques and Cryptococcus neoformans (C. neoformans) was isolated from maculopapular lesions on body parts (face, hands and chest) and body fluids (urine, expectorated sputum, and cerebrospinal fluid). In vitro drug susceptibility testing on the yeast isolates demonstrated resistance to fluconazole acquired by C. tropicalis which was a suggestive possible root cause of recurrent oral candidiasis in this patient.

scholarly journals Pneumoperitoneum in in-vitro Conceived Quadruplet Neonate: Rare Manifestation of Hirschsprungʼs Disease – Report of a Case

2015 ◽  
Vol 58 (4) ◽  
pp. 144-146
Author(s):  
Jakov Mihanović ◽  
Ivo Jurić ◽  
Zenon Pogorelić ◽  
Ivana Mrklić ◽  
Miro Jukić ◽  
...  
Keyword(s):  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Premature Neonate ◽  
Multiple Pregnancies ◽  
Case Presentation ◽  
Spontaneous Pneumoperitoneum ◽  
Rare Manifestation

Introduction: Hirschsprung’s disease is a congenital colonic aganglionosis, usually presented as inability or difficulty in passing of meconium, chronic and persistent obstipation, maleficent feeding, vomiting, distension and lethargy. Case presentation: We presented a case of an in-vitro conceived quadruplet premature neonate who presented with pneumoperitoneum caused by transverse colon spontaneous perforation and microcolon appearance of distal bowel, treated by resection and temporary colostomy turns to be a rare manifestation of Hirschsprung’s disease. Conclusion: Assisted reproductive technologies increases chances for multiple pregnancies and may increase chance for major congenital anomalies. Rare manifestation of Hirschsprung’s disease is spontaneous pneumoperitoneum which remains a surgical emergency. Delay in recognizing and treatment can significantly worsen prognosis. In neonate with intestinal perforation one should consider Hirschsprung’s disease.

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