Whole-genomic analysis of a human G1P[9] rotavirus strain reveals intergenogroup-reassortment events

Group A rotavirus (RVA) strain K8 (RVA/Human-tc/JPN/K8/1977/G1P[9]) was found to have Wa-like VP7 and NSP1 genes and AU-1-like VP4 and NSP5 genes. To determine the exact origin and overall genetic makeup of this unusual RVA strain, the remaining genes (VP1–VP3, VP6 and NSP2–NSP4) of K8 were analysed in this study. Strain K8 exhibited a G1-P[9]-I1-R3-C3-M3-A1-N1-T3-E3-H3 genotype constellation, not reported previously. The VP6 and NSP2 genes of strain K8 were related closely to those of common human Wa-like G1P[8] and/or G3P[8] strains, whilst its VP1–VP3, NSP3 and NSP4 genes were related more closely to those of AU-1-like RVAs and/or AU-1-like genes of multi-reassortant strains than to those of other RVAs. Therefore, strain K8 might have originated from intergenogroup-reassortment events involving acquisition of four Wa-like genes, possibly from G1P[8] RVAs, by an AU-1-like P[9] strain. Whole-genomic analysis of strain K8 has provided important insights into the complex genetic diversity of RVAs.

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Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

Pathogens ◽

10.3390/pathogens10060674 ◽

2021 ◽

Vol 10 (6) ◽

pp. 674

Author(s):

Anton Yuzhakov ◽

Ksenia Yuzhakova ◽

Nadezhda Kulikova ◽

Lidia Kisteneva ◽

Stanislav Cherepushkin ◽

...

Keyword(s):

Infectious Disease ◽

Genetic Diversity ◽

Acute Gastroenteritis ◽

Rt Pcr ◽

Clinical Hospital ◽

Group A Rotavirus ◽

Group A ◽

First Time ◽

Common Combination ◽

Children Under 5

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

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Isolation and characterization of a rare group A rotavirus G13P[18] strain from a diarrhoeic foal in Japan

Journal of General Virology ◽

10.1099/jgv.0.001437 ◽

2020 ◽

Vol 101 (8) ◽

pp. 800-805

Author(s):

Manabu Nemoto ◽

Hidekazu Niwa ◽

Hiroshi Kida ◽

Tohru Higuchi ◽

Yasuhiro Orita ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Group A Rotavirus ◽

Isolation And Characterization ◽

Genotype Constellation ◽

Group A ◽

First Time ◽

Rare Group

A rare genotype G13P[18] group A rotavirus (RVA/Horse-tc/JPN/MK9/2019/G13P[18]) was isolated from a diarrhoeic foal for the first time in 28 years. The genotype constellation of the virus was assigned to G13-P[18]-I6-R9-C9-M6-A6-N9-T12-E14-H11 and was the same as that of the first isolated strain, RVA/Horse-tc/GBR/L338/1991/G13P[18]. Phylogenetic analysis suggests that the virus is related to RVA/Horse-tc/GBR/L338/1991/G13P[18] and is distant from typical equine rotaviruses of the G3P[12] and G14P[12] genotypes.

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Full genomic analysis of a human group A rotavirus G9P[6] strain from Eastern India provides evidence for porcine-to-human interspecies transmission

Archives of Virology ◽

10.1007/s00705-009-0363-3 ◽

2009 ◽

Vol 154 (5) ◽

pp. 733-746 ◽

Cited By ~ 43

Author(s):

Anupam Mukherjee ◽

Dipanjan Dutta ◽

Souvik Ghosh ◽

Parikshit Bagchi ◽

Shiladitya Chattopadhyay ◽

...

Keyword(s):

Genomic Analysis ◽

Interspecies Transmission ◽

Eastern India ◽

Human Group ◽

Group A Rotavirus ◽

Group A

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Corrigendum to “Genetic diversity of porcine group A rotavirus strains in the UK” [Vet. Microbiol. 173 (2014) 27–37]

Veterinary Microbiology ◽

10.1016/j.vetmic.2014.10.003 ◽

2014 ◽

Vol 174 (3-4) ◽

pp. 609

Author(s):

Rebecca Chandler-Bostock ◽

Laura R. Hancox ◽

Sameena Nawaz ◽

Oliver Watts ◽

Miren Iturriza-Gomara ◽

...

Keyword(s):

Genetic Diversity ◽

Group A Rotavirus ◽

Group A ◽

The Uk

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Molecular characterization of group A rotavirus strains detected in alpacas (Vicugna pacos) from Peru

Journal of General Virology ◽

10.1099/jgv.0.001501 ◽

2021 ◽

Vol 102 (4) ◽

Author(s):

Alejandra Badaracco ◽

Aida Cordero ◽

Celina Vega ◽

Marina Bok ◽

Mark Zeller ◽

...

Keyword(s):

Economic Resource ◽

Group A Rotavirus ◽

The Third ◽

Antigen Capture ◽

Vicugna Pacos ◽

Faecal Samples ◽

Genotype Constellation ◽

Group A ◽

Main Producer

The alpaca is a very important social and economic resource for the production of fibre and meat for Andean communities. Peru is the main producer of alpacas. Group A rotavirus (RVA) has been sporadically detected in alpacas. In this study, a total of 1423 faecal samples from alpacas from different locations of the Puno department in Peru were collected and analysed by an antigen-capture ELISA in order to detect RVA. Four per cent of the samples were RVA-positive (57/1423). The genotype constellation of three selected alpaca RVA strains were G3/8 P[1/14]-I2-R2/5-C2/3-M2/3-A17-N2/3-T6-E3-H3. Two of the analysed strains presented a bovine-like genotype constellation, whereas the third strain presented six segments belonging to the AU-1-like genogroup (G3, M3, C3, N3, T3 and E3), suggesting reassorting events. Monitoring of the sanitary health of juvenile alpacas is essential to reduce the rates of neonatal mortality and for the development of preventive health strategies.

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Genetic diversity of porcine group A rotavirus strains in the UK

Veterinary Microbiology ◽

10.1016/j.vetmic.2014.06.030 ◽

2014 ◽

Vol 173 (1-2) ◽

pp. 27-37 ◽

Cited By ~ 14

Author(s):

Rebecca Chandler-Bostock ◽

Laura R. Hancox ◽

Sameena Nawaz ◽

Oliver Watts ◽

Miren Iturriza-Gomara ◽

...

Keyword(s):

Genetic Diversity ◽

Group A Rotavirus ◽

Group A ◽

The Uk

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Group A rotavirus gastroenteritis: post-vaccine era, genotypes and zoonotic transmission

Einstein (São Paulo) ◽

10.1590/s1679-45082016rb3582 ◽

2016 ◽

Vol 14 (2) ◽

pp. 278-287 ◽

Cited By ~ 18

Author(s):

Adriana Luchs ◽

Maria do Carmo Sampaio Tavares Timenetsky

Keyword(s):

Genetic Diversity ◽

Diarrheal Disease ◽

Zoonotic Transmission ◽

Interspecies Transmission ◽

Clinical Aspects ◽

Human Populations ◽

Group A Rotavirus ◽

Rotavirus Gastroenteritis ◽

Group A ◽

Rna Genome

ABSTRACT This article provides a review of immunity, diagnosis, and clinical aspects of rotavirus disease. It also informs about the changes in epidemiology of diarrheal disease and genetic diversity of circulating group A rotavirus strains following the introduction of vaccines. Group A rotavirus is the major pathogen causing gastroenteritis in animals. Its segmented RNA genome can lead to the emergence of new or unusual strains in human populations via interspecies transmission and/or reassortment events.

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