scholarly journals The proline-rich region of the ecotropic Moloney murine leukaemia virus envelope protein tolerates the insertion of the green fluorescent protein and allows the generation of replication-competent virus

2003 ◽  
Vol 84 (2) ◽  
pp. 369-373 ◽  
Author(s):  
Otto Erlwein ◽  
Christian J. Buchholz ◽  
Barbara S. Schnierle
Keyword(s):  
Green Fluorescent Protein ◽  
Envelope Protein ◽  
Fluorescent Protein ◽  
Virus Envelope ◽  
Rich Region ◽  
Leukaemia Virus ◽  
Virus Envelope Protein ◽  
Green Fluorescent ◽  
Moloney Murine Leukaemia Virus ◽  
Proline Rich Region

scholarly journals Two Point Mutations Produce Infectious Retrovirus Bearing a Green Fluorescent Protein-SU Fusion Protein

2001 ◽  
Vol 75 (23) ◽  
pp. 11881-11885 ◽  
Author(s):  
Krishnakumar Kizhatil ◽  
Adam Gromley ◽  
Lorraine M. Albritton
Keyword(s):  
Green Fluorescent Protein ◽  
Envelope Protein ◽  
Structural Changes ◽  
Fluorescent Protein ◽  
Fundamental Problem ◽  
Transmembrane Protein ◽  
Strong Association ◽  
Slight Reduction ◽  
Virus Envelope ◽  
Green Fluorescent

ABSTRACT Two second-site mutations in Moloney murine leukemia virus envelope surface protein (SU) were previously shown to rescue infection of two different SU mutants, a fusion-defective point mutant and a fusion-defective modified SU that exhibits weak subunit association. We report here that they also rescue infection of a third defective SU, one modified by insertion of the green fluorescent protein (GFP) between serine 6 and proline 7. GFP-SU assembled into virions and showed a strong association with the transmembrane protein (TM). However, these virions were noninfectious. GFP-SU expression was not maintained within cells, suggesting that the protein was toxic. Addition of the second-site mutations rendered the GFP-SU virus infectious and resulted in prolonged expression of the modified envelope protein. This virus showed a slight reduction in receptor binding but not in envelope protein processing, suggesting that addition of the GFP sequences results in subtle structural changes. Extrapolating these data, we see that the fundamental problem with the GFP-SU envelope protein appears to be a folding problem, suggesting that the second-site mutations rescue GFP-SU primarily by a mechanism that involves stabilizing the envelope protein structure.

Baculoviral Display of the Green Fluorescent Protein and Rubella Virus Envelope Proteins

1997 ◽  
Vol 238 (3) ◽  
pp. 717-722 ◽  
Author(s):  
David Mottershead ◽  
Inge van der Linden ◽  
Carl-Henrik von Bonsdorff ◽  
Kari Keinänen ◽  
Christian Oker-Blom
Keyword(s):  
Green Fluorescent Protein ◽  
Rubella Virus ◽  
Fluorescent Protein ◽  
Envelope Proteins ◽  
Virus Envelope ◽  
Green Fluorescent

scholarly journals Green Fluorescent Protein-Tagged Retroviral Envelope Protein for Analysis of Virus-Cell Interactions

2003 ◽  
Vol 77 (10) ◽  
pp. 6070-6075 ◽  
Author(s):  
Dirk Spitzer ◽  
Kurt E. J. Dittmar ◽  
Manfred Rohde ◽  
Hansjörg Hauser ◽  
Dagmar Wirth
Keyword(s):  
Green Fluorescent Protein ◽  
Envelope Protein ◽  
Fluorescent Protein ◽  
Inverse Correlation ◽  
Cell Interactions ◽  
Virus Particles ◽  
Viral Particles ◽  
Infected Cells ◽  
Env Protein ◽  
Green Fluorescent

ABSTRACT Fluorescent retroviral envelope (Env) proteins were developed for direct visualization of viral particles. By fusing the enhanced green fluorescent protein (eGFP) to the N terminus of the amphotropic 4070A envelope protein, extracellular presentation of eGFP was achieved. Viruses incorporated the modified Env protein and efficiently infected cells. We used the GFP-tagged viruses for staining retrovirus receptor-positive cells, thereby circumventing indirect labeling techniques. By generating cells which conditionally expressed the GFP-tagged Env protein, we could confirm an inverse correlation between retroviral Env expression and infectivity (superinfection). eGFP-tagged virus particles are suitable for monitoring the dynamics of virus-cell interactions.

scholarly journals Functional incorporation of green fluorescent protein into hepatitis B virus envelope particles

Virology ◽  
2004 ◽  
Vol 330 (1) ◽  
pp. 158-167 ◽  
Author(s):  
Carsten Lambert ◽  
Nicole Thomé ◽  
Christoph J. Kluck ◽  
Reinhild Prange
Keyword(s):  
Green Fluorescent Protein ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Fluorescent Protein ◽  
Virus Envelope ◽  
B Virus ◽  
Green Fluorescent

The Green Fluorescent Protein (GFP)

2013 ◽  
Vol 05 (01) ◽  
pp. 101-102
Author(s):  
Phunlerd Piyaraj
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent
Sign in / Sign up

Export Citation Format

Share Document