scholarly journals Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replication

2003 ◽  
Vol 84 (4) ◽  
pp. 781-788 ◽  
Author(s):  
Heng Zhao ◽  
Ben P. H. Peeters
Keyword(s):  
Gene Expression ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Virus Replication ◽  
Newcastle Disease ◽  
Viral Vector ◽  
Foreign Gene ◽  
Genomic Location ◽  
Recombinant Newcastle Disease Virus

Gene expression profiling in PC12 cells infected with an oncolytic Newcastle disease virus strain

2014 ◽  
Vol 185 ◽  
pp. 10-22 ◽  
Author(s):  
András Balogh ◽  
Judit Bátor ◽  
Lajos Markó ◽  
Mária Németh ◽  
Marianna Pap ◽  
...  
Keyword(s):  
Gene Expression ◽  
Newcastle Disease Virus ◽  
Gene Expression Profiling ◽  
Pc12 Cells ◽  
Disease Virus ◽  
Virus Strain ◽  
Newcastle Disease ◽  
Expression Profiling ◽  
Newcastle Disease Virus Strain

Protection and Differentiation of Infected from Vaccinated Animals by an Inactivated Recombinant Newcastle Disease Virus/Avian Influenza H5 Vaccine

2010 ◽  
Vol 5 (s1) ◽  
pp. e23-e24
Author(s):  
Bernardo Lozano-Dubernard ◽  
Ernesto Soto-Priante ◽  
David Sarfati-Mizrahi ◽  
Felipa Castro-Peralta ◽  
Ricardo Flores-Castro ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Newcastle Disease Virus

scholarly journals High-level expression of a foreign gene from the most 3′-proximal locus of a recombinant Newcastle disease virus

2001 ◽  
Vol 82 (7) ◽  
pp. 1729-1736 ◽  
Author(s):  
Zhuhui Huang ◽  
Sateesh Krishnamurthy ◽  
Aruna Panda ◽  
Siba K. Samal
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vaccine Vector ◽  
Foreign Gene ◽  
Gene Encoding ◽  
Reading Frame ◽  
Virulent Newcastle Disease Virus ◽  
Nucleocapsid Protein Gene ◽  
High Level

A previous report showed that insertion of a foreign gene encoding chloramphenicol acetyltransferase (CAT) between the HN and L genes of the full-length cDNA of a virulent Newcastle disease virus (NDV) yielded virus with growth retardation and attenuation. The NDV vector used in that study was pathogenic to chickens; it is therefore not suitable for use as a vaccine vector. In the present study, an avirulent NDV vector was generated and its potential to express CAT protein was evaluated. The CAT gene was under the control of NDV transcriptional start and stop signals and was inserted immediately before the open reading frame of the viral 3′-proximal nucleocapsid protein gene. A recombinant NDV expressing CAT activity at a high level was recovered. The replication and pathogenesis of the CAT-expressing recombinant NDV were not modified significantly. These results indicate the potential utility of an avirulent NDV as a vaccine vector.

scholarly journals An improved method for the rescue of recombinant Newcastle disease virus

BioTechniques ◽  
2020 ◽  
Vol 68 (2) ◽  
pp. 96-100
Author(s):  
Pheik-Sheen Cheow ◽  
Tiong Kit Tan ◽  
Adelene Ai-Lian Song ◽  
Khatijah Yusoff ◽  
Suet Lin Chia
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Vaccine Development ◽  
Transfection Efficiency ◽  
Transfection Method ◽  
Helper Plasmids ◽  
Immunogenic Properties ◽  
Recombinant Newcastle Disease Virus

Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfection efficiency of the four plasmids. The virus progenies produced were quantitated with flow cytometry analysis of the infectious virus unit. The modified transfection method increased the titer of virus progenies compared with that of the standard transfection method.

Rescue of a recombinant Newcastle disease virus strain R2B expressing green fluorescent protein

Virus Genes ◽  
2017 ◽  
Vol 53 (3) ◽  
pp. 410-417 ◽  
Author(s):  
Madhan Mohan Chellappa ◽  
Sohini Dey ◽  
Satish Gaikwad ◽  
Dinesh C. Pathak ◽  
Vikram N. Vakharia
Keyword(s):  
Green Fluorescent Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Virus Strain ◽  
Newcastle Disease ◽  
Fluorescent Protein ◽  
Newcastle Disease Virus Strain ◽  
Green Fluorescent ◽  
Recombinant Newcastle Disease Virus
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