scholarly journals Development of a Newcastle disease virus vector expressing a foreign gene through an internal ribosomal entry site provides direct proof for a sequential transcription mechanism

2015 ◽  
Vol 96 (8) ◽  
pp. 2028-2035 ◽  
Author(s):  
Zhenyu Zhang ◽  
Wei Zhao ◽  
Deshan Li ◽  
Jinlong Yang ◽  
Laszlo Zsak ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Internal Ribosomal Entry Site ◽  
Direct Proof ◽  
Virus Vector ◽  
Foreign Gene ◽  
Entry Site ◽  
Ribosomal Entry

scholarly journals Expression of Two Foreign Genes from the Optimal Insertion Sites of Newcastle Disease Virus Vector for Use as a Multivalent Vaccine and Gene Therapy Vector

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 9
Author(s):  
Lei He ◽  
Zhenyu Zhang ◽  
Qingzhong Yu
Keyword(s):  
Gene Therapy ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Fluorescent Protein ◽  
Transcription Unit ◽  
Lower Amount ◽  
Foreign Gene ◽  
Entry Site ◽  
Insertion Sites

Many Newcastle disease virus (NDV) strains have been developed as vectors to express a foreign gene (FG) for vaccine and gene therapy purposes. A majority of these NDV vectors express only a single FG or two FGs from suboptimal insertion sites in the NDV genome, obtaining various levels of FG expression. To improve the FG expression, we generated NDV LaSota vaccine strain-based recombinant viruses to express two FGs, green fluorescent protein (GFP) and red fluorescent protein (RFP) genes, from the identified optimal insertion sites, through a combination of the independent transcription unit (ITU) and the internal ribosomal entry site (IRES) dependent expression approaches. Biological assessments showed that these recombinants expressing two FGs were slightly attenuated with approximately one order of magnitude lower in virus titers than those containing a single FG. The FG expression efficiencies from two-FG viruses were also lower than those from the single-FG viruses. However, the expression of two FGs from the optimal insertion sites was significantly (p < 0.05) higher than those from the suboptimal insertion sites. The expression of FGs through the ITU approach was approximately 4-fold more efficient than that through the IRES-dependent approach. These results suggest that the NDV LaSota vector could efficiently express two FGs from the identified optimal insertions sites. The ITU strategy could be used for the expression of a higher amount of FG products, whereas the IRES tactic might be useful when a lower amount of FG products are needed.

scholarly journals High-level expression of a foreign gene from the most 3′-proximal locus of a recombinant Newcastle disease virus

2001 ◽  
Vol 82 (7) ◽  
pp. 1729-1736 ◽  
Author(s):  
Zhuhui Huang ◽  
Sateesh Krishnamurthy ◽  
Aruna Panda ◽  
Siba K. Samal
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vaccine Vector ◽  
Foreign Gene ◽  
Gene Encoding ◽  
Reading Frame ◽  
Virulent Newcastle Disease Virus ◽  
Nucleocapsid Protein Gene ◽  
High Level

A previous report showed that insertion of a foreign gene encoding chloramphenicol acetyltransferase (CAT) between the HN and L genes of the full-length cDNA of a virulent Newcastle disease virus (NDV) yielded virus with growth retardation and attenuation. The NDV vector used in that study was pathogenic to chickens; it is therefore not suitable for use as a vaccine vector. In the present study, an avirulent NDV vector was generated and its potential to express CAT protein was evaluated. The CAT gene was under the control of NDV transcriptional start and stop signals and was inserted immediately before the open reading frame of the viral 3′-proximal nucleocapsid protein gene. A recombinant NDV expressing CAT activity at a high level was recovered. The replication and pathogenesis of the CAT-expressing recombinant NDV were not modified significantly. These results indicate the potential utility of an avirulent NDV as a vaccine vector.

scholarly journals Correction: Comparative Immunogenicity of HIV-1 gp160, gp140 and gp120 Expressed by Live Attenuated Newcastle Disease Virus Vector

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
Author(s):  
Sunil K. Khattar ◽  
Sweety Samal ◽  
Celia C. LaBranche ◽  
David C. Montefiori ◽  
Peter L. Collins ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virus Vector ◽  
Hiv 1

scholarly journals Recovery of a Virulent Strain of Newcastle Disease Virus from Cloned cDNA: Expression of a Foreign Gene Results in Growth Retardation and Attenuation

Virology ◽  
2000 ◽  
Vol 278 (1) ◽  
pp. 168-182 ◽  
Author(s):  
Sateesh Krishnamurthy ◽  
Zhuhui Huang ◽  
Siba K. Samal
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Growth Retardation ◽  
Newcastle Disease ◽  
Virulent Strain ◽  
Foreign Gene ◽  
Cdna Expression ◽  
Cloned Cdna

scholarly journals Identification of Optimal Insertion Site in Recombinant Newcastle Disease Virus (rNDV) Vector Expressing Foreign Gene to Enhance Its Anti-Tumor Effect

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0164723 ◽  
Author(s):  
Ziye Pan ◽  
Jinjiao He ◽  
Lubna M. Rasoul ◽  
Yunye Liu ◽  
Ruixiang Che ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Insertion Site ◽  
Foreign Gene ◽  
Recombinant Newcastle Disease Virus
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