scholarly journals Human herpesvirus 6 envelope cholesterol is required for virus entry

2006 ◽  
Vol 87 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Honglan Huang ◽  
Yongmei Li ◽  
Tomohiko Sadaoka ◽  
Huanmin Tang ◽  
Takahito Yamamoto ◽  
...  
Keyword(s):  
Cell Fusion ◽  
Viral Entry ◽  
Virus Entry ◽  
Human Herpesvirus ◽  
Fusion Process ◽  
Cholesterol Depletion ◽  
Human Herpesvirus 6 ◽  
Exogenous Cholesterol ◽  
Herpesvirus 6

In this study, the role of cholesterol in the envelope of human herpesvirus 6 (HHV-6) was examined by using methyl-β-cyclodextrin (MβCD) depletion. When cholesterol was removed from HHV-6 virions with MβCD, infectivity was abolished, but it could be rescued by the addition of exogenous cholesterol. HHV-6 binding was affected slightly by MβCD treatment. In contrast, envelope cholesterol depletion markedly affected HHV-6 infectivity and HHV-6-induced cell fusion. These results suggest that the cholesterol present in the HHV-6 envelope plays a prominent role in the fusion process and is a key component in viral entry.

scholarly journals Role of dendritic cells infected with human herpesvirus 6 in virus transmission to CD4+ T cells

Virology ◽  
2009 ◽  
Vol 385 (2) ◽  
pp. 294-302 ◽  
Author(s):  
Masaya Takemoto ◽  
Takayoshi Imasawa ◽  
Koichi Yamanishi ◽  
Yasuko Mori
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Cd4 T Cells ◽  
Virus Transmission ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6

The role of human herpesvirus-6 and inflammatory markers in the pathogenesis of multiple sclerosis

2020 ◽  
Vol 346 ◽  
pp. 577313 ◽  
Author(s):  
Hossein Keyvani ◽  
Hamid Zahednasab ◽  
Hussain ali abraham Aljanabi ◽  
Muhammad Asadi ◽  
Rasoul Mirzaei ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Inflammatory Markers ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6

Inheritance of Chromosomally Integrated Human Herpesvirus 6 DNA

Blood ◽  
1999 ◽  
Vol 94 (5) ◽  
pp. 1545-1549 ◽  
Author(s):  
Masanori Daibata ◽  
Takahiro Taguchi ◽  
Yuiko Nemoto ◽  
Hirokuni Taguchi ◽  
Isao Miyoshi
Keyword(s):  
Dna Sequences ◽  
Viral Genome ◽  
Family Members ◽  
Human Herpesvirus ◽  
Single Step ◽  
Human Herpesvirus 6 ◽  
Viral Genomes ◽  
Herpesvirus 6

Abstract Human herpesvirus 6 (HHV-6) genome has been detected in several human lymphoproliferative disorders with no signs of active viral infection, and found to be integrated into chromosomes in some cases. We previously reported a woman with HHV-6–infected Burkitt’s lymphoma. Fluorescence in situ hybridization showed that the viral genome was integrated into the long arm of chromosome 22 (22q13). The patient’s asymptomatic husband also carried HHV-6 DNA integrated at chromosome locus 1q44. To assess the possibility of chromosomal transmission of HHV-6 DNA, we looked for HHV-6 DNA in the peripheral blood of their daughter. She had HHV-6 DNA on both chromosomes 22q13 and 1q44, identical to the site of viral integration of her mother and father, respectively. The findings suggested that her viral genomes were inherited chromosomally from both parents. The 3 family members were all seropositive for HHV-6, but showed no serological signs of active infection. To confirm the presence of HHV-6 DNA sequences, we performed polymerase chain reaction (PCR) with 7 distinct primer pairs that target different regions of HHV-6. The viral sequences were consistently detected by single-step PCR in all 3 family members. We propose a novel latent form for HHV-6, in which integrated viral genome can be chromosomally transmitted. The possible role of the chromosomally integrated HHV-6 in the pathogenesis of lymphoproliferative diseases remains to be explained.

Inheritance of Chromosomally Integrated Human Herpesvirus 6 DNA

Blood ◽  
1999 ◽  
Vol 94 (5) ◽  
pp. 1545-1549 ◽  
Author(s):  
Masanori Daibata ◽  
Takahiro Taguchi ◽  
Yuiko Nemoto ◽  
Hirokuni Taguchi ◽  
Isao Miyoshi
Keyword(s):  
Dna Sequences ◽  
Viral Genome ◽  
Family Members ◽  
Human Herpesvirus ◽  
Single Step ◽  
Human Herpesvirus 6 ◽  
Viral Genomes ◽  
Herpesvirus 6

Human herpesvirus 6 (HHV-6) genome has been detected in several human lymphoproliferative disorders with no signs of active viral infection, and found to be integrated into chromosomes in some cases. We previously reported a woman with HHV-6–infected Burkitt’s lymphoma. Fluorescence in situ hybridization showed that the viral genome was integrated into the long arm of chromosome 22 (22q13). The patient’s asymptomatic husband also carried HHV-6 DNA integrated at chromosome locus 1q44. To assess the possibility of chromosomal transmission of HHV-6 DNA, we looked for HHV-6 DNA in the peripheral blood of their daughter. She had HHV-6 DNA on both chromosomes 22q13 and 1q44, identical to the site of viral integration of her mother and father, respectively. The findings suggested that her viral genomes were inherited chromosomally from both parents. The 3 family members were all seropositive for HHV-6, but showed no serological signs of active infection. To confirm the presence of HHV-6 DNA sequences, we performed polymerase chain reaction (PCR) with 7 distinct primer pairs that target different regions of HHV-6. The viral sequences were consistently detected by single-step PCR in all 3 family members. We propose a novel latent form for HHV-6, in which integrated viral genome can be chromosomally transmitted. The possible role of the chromosomally integrated HHV-6 in the pathogenesis of lymphoproliferative diseases remains to be explained.

scholarly journals ROLE OF HUMAN HERPESVIRUS-6 IN THE PATHOGENESIS OF FUNGAL INFECTIONS AND IMPACT ON OUTCOME IN LIVER TRANSPLANT RECIPIENTS.

2000 ◽  
Vol 69 (Supplement) ◽  
pp. S406
Author(s):  
Jeffrey Rogers ◽  
Nina Singh ◽  
Susan Rohal ◽  
Shimon Kusne ◽  
Donald R. Carrigan ◽  
...  
Keyword(s):  
Liver Transplant ◽  
Fungal Infections ◽  
Human Herpesvirus ◽  
Transplant Recipients ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6 ◽  
Liver Transplant Recipients

scholarly journals The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope

2003 ◽  
Vol 77 (4) ◽  
pp. 2452-2458 ◽  
Author(s):  
Yasuko Mori ◽  
Pilailuk Akkapaiboon ◽  
Xuwei Yang ◽  
Koichi Yamanishi
Keyword(s):  
Viral Entry ◽  
Human Herpesvirus ◽  
Viral Envelope ◽  
Gene Products ◽  
Human Herpesvirus 6 ◽  
Glycoprotein Complex ◽  
The Third ◽  
Glycoprotein H ◽  
Infected Cells ◽  
Herpesvirus 6

ABSTRACT The human herpesvirus 6 (HHV-6) variant A U100 gene encodes the third component of the glycoprotein H (gH)-glycoprotein L (gL)-containing complex. Glycosidase digestion analysis showed that the U100 gene products are glycoproteins consisting of an 80-kDa protein with complex N-linked oligosaccharides and a 74-kDa protein with immature, high-mannose N-linked oligosaccharides. Based on these characteristics, we designated the U100 gene products glycoprotein Q (gQ). Only the 80-kDa form of gQ was coimmunoprecipitated with an anti-gH antibody, suggesting that the 80-kDa protein associates with the gH-gL complex in HHV-6-infected cells. Furthermore, the complex was detected in purified virions, suggesting that it may play an important role in viral entry.

Role of the Human Herpesvirus 6 U69-Encoded Kinase in the Phosphorylation of Ganciclovir

2002 ◽  
Vol 62 (3) ◽  
pp. 714-721 ◽  
Author(s):  
Leen De Bolle ◽  
Detlef Michel ◽  
Thomas Mertens ◽  
Chaysavanh Manichanh ◽  
Henri Agut ◽  
...  
Keyword(s):  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6
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