scholarly journals Supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in India

2008 ◽  
Vol 89 (3) ◽  
pp. 818-828 ◽  
Author(s):  
S. Chakraborty ◽  
R. Vanitharani ◽  
B. Chattopadhyay ◽  
C. M. Fauquet
Keyword(s):  
Distinct Species ◽  
Tomato Leaf ◽  
New Delhi ◽  
Symptom Development ◽  
Tomato Leaf Curl Disease ◽  
Northern India ◽  
Viral Dnas ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Isolates of two distinct begomovirus species, the severe strain of the species Tomato leaf curl New Delhi virus (tomato leaf curl New Delhi virus-[India:New Delhi:Severe:1992]; ToLCNDV-[IN:ND:Svr:92], bipartite) and the Varanasi strain of the species Tomato leaf curl Gujarat virus (tomato leaf curl Gujarat virus-[India:Varanasi:2001]; ToLCGV-[IN:Var:01], mono/bipartite) infect tomato (Lycopersicon esculentum) and cause severe yield losses in northern India. This study investigated the infectivity properties of genomic components of these two species. Both pseudorecombinants were infectious in Nicotiana benthamiana, Nicotiana tabacum and L. esculentum. Enhanced pathogenicity was observed when DNA-A of ToLCNDV-[IN:ND:Svr:92] was trans-complemented with ToLCGV-[IN:Var:01] DNA-B, and was consistently associated with an increase in accumulation of ToLCGV-[IN:Var:01] DNA-B. Mixed infection of ToLCNDV-[IN:ND:Svr:92] and ToLCGV-[IN:Var:01] always showed extremely severe symptoms, suggesting a synergistic interaction between these two viruses. Southern blot analysis of viral DNAs from infected plants showed a significantly higher level of accumulation of both ToLCNDV components and DNA-B of ToLCGV-[IN:Var:01] with no alteration to levels of DNA-A of ToLCGV-[IN:Var:01]. Symptom development and/or higher infectivity of the supervirulent pseudorecombinants correlated with the increased levels of DNA-B accumulation. Protoplast replication assays revealed that enhanced infectivity by the pseudorecombinant occurred at the level of replication, as DNA-A of ToLCNDV-[IN:ND:Svr:92] enhanced ToLCGV-[IN:Var:01] DNA-B replication, whose accumulation was in turn increased by ToLCGV-[IN:Var:01] DNA-A. This is the first report demonstrating a virulent pseudorecombinant between two distinct species of begomoviruses that infect tomato, and is the second report on synergism between begomoviruses. The results revealed that ToLCGV-[IN:Var:01] DNA-B is capable of associating with different DNA-A components, despite having different iteron sequences.

Potato apical leaf curl disease: current status and perspectives on a disease caused by tomato leaf curl New Delhi virus

2021 ◽  
Author(s):  
Ravinder Kumar ◽  
Rahul Kumar Tiwari ◽  
Arjunan Jeevalatha ◽  
Sundaresha Siddappa ◽  
Mohd. Abas Shah ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Current Status ◽  
New Delhi ◽  
Apical Leaf ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Diversity analysis of Tomato leaf curl New Delhi virus-[potato], causing apical leaf curl disease of potato in India

2017 ◽  
Vol 45 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Arjunan Jeevalatha ◽  
Swarup Kumar Chakrabarti ◽  
Sanjeev Sharma ◽  
Vinay Sagar ◽  
Kamlesh Malik ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Diversity Analysis ◽  
New Delhi ◽  
Apical Leaf ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals Molecular Characterization of a Begomovirus, α-Satellite, and β-Satellite Associated with Leaf Curl Disease of Parthenium hysterophorus in India

Plant Disease ◽  
2016 ◽  
Vol 100 (11) ◽  
pp. 2299-2305 ◽  
Author(s):  
Susheel Kumar ◽  
Ashish Srivastava ◽  
Meraj Jaidi ◽  
Puneet Singh Chauhan ◽  
S. K. Raj
Keyword(s):  
Satellite Dna ◽  
Rolling Circle Amplification ◽  
Tomato Leaf ◽  
Parthenium Hysterophorus ◽  
Alternate Host ◽  
Invasive Weed ◽  
Tomato Leaf Curl Disease ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Parthenium hysterophorus plants exhibiting severe leaf curl and stunting symptoms were observed near agriculture fields in Lucknow, India. The association of a begomovirus, β-satellite, and α-satellite with these symptoms of a Parthenium disease was investigated by sequence analyses of virus and satellite DNA amplified by rolling circle amplification and polymerase chain reaction. The highest sequence identities and closest phylogenetic relationships for the begomovirus, β-satellite, and α-satellite detected in P. hysterophorus plants were to Tomato leaf curl virus (ToLCV), papaya leaf curl β-satellite (PaLCuB), and Ageratum yellow vein India α-satellite (AYVIA), respectively. These findings identified the virus and satellites infecting the Parthenium sp. as ToLCV, PaLCuB, and AYVIA, respectively. P. hysterophorus and tomato seedlings infected with cloned ToLCV, PaLCuB, and AYVIA by agroinoculation developed leaf curl symptoms, whereas plants infected with ToLCV alone or with ToLCV and AYVIA developed mild yellowing. The results show that this complex infects and causes disease in P. hysterophorus and tomato. P. hysterophorus is an invasive weed commonly found around agricultural fields and along roadsides in India. These results indicate that P. hysterophorus plants infected with ToLCV and associated satellite DNA act as an alternate host (reservoir), and that could lead to increased incidence of tomato leaf curl disease.

Performance of Some Cultivars of Tomato Against Tomato Leaf Curl Disease

2001 ◽  
Vol 4 (5) ◽  
pp. 528-530
Author(s):  
Afia Akhtar . ◽  
M. A. Rahman khokon . ◽  
Bimal Kumar Pramanik . ◽  
M. Ashrafuzzaman .
Keyword(s):  
Tomato Leaf ◽  
Tomato Leaf Curl Disease ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Molecular Variability in the Replicase Gene of Viruses Causing Tomato Leaf Curl Disease in India

2004 ◽  
Vol 13 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Subodh Kumar Sinha ◽  
Arupratan Dasgupta ◽  
Anita Baranwal ◽  
Shelly Praveen
Keyword(s):  
Tomato Leaf ◽  
Replicase Gene ◽  
Molecular Variability ◽  
Tomato Leaf Curl Disease ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Association of chili leaf curl betasatellite with tomato leaf curl disease

2017 ◽  
Vol 83 (6) ◽  
pp. 402-405 ◽  
Author(s):  
Muhammad Qasim Aslam ◽  
Khalid P. Akhtar ◽  
Mohy-u-Din Akram ◽  
Muhammad Y. Saleem ◽  
Nighat Sarwar ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Tomato Leaf Curl Disease ◽  
Chili Leaf Curl Betasatellite ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals A New Begomovirus Species Causing Tomato Leaf Curl Disease in Varanasi, India

Plant Disease ◽  
2003 ◽  
Vol 87 (3) ◽  
pp. 313-313 ◽  
Author(s):  
S. Chakraborty ◽  
P. K. Pandey ◽  
M. K. Banerjee ◽  
G. Kalloo ◽  
C. M. Fauquet
Keyword(s):  
Infectious Clone ◽  
Uttar Pradesh ◽  
Tomato Leaf ◽  
Full Length ◽  
Degenerate Primers ◽  
Tomato Leaf Curl Disease ◽  
Leaf Curling ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

In November 2001, a leaf curl disease of tomato, manifested by yellowing of leaf lamina, upward leaf curling, leaf distortion, shrinking of leaf surface, and stunted plant growth was observed in tomato-growing areas in the Varanasi and Mirzapur districts of eastern Uttar Pradesh, India, which caused yield losses up to 100%. The causal agent was infective to tomato cv. Punjab Chuhara by whiteflies and grafting. Inoculated plants developed symptoms observed in naturally infected tomatoes. Viral DNA was isolated from artificially inoculated tomato plants using 1% CTAB (2) followed by a concentration of supercoiled DNA by alkaline denaturation (1). A geminivirus was confirmed by polymerase chain reaction using DNA-A degenerate primers (3), and a 550-bp amplified product was obtained from artificially and naturally infected plants. Full-length viral genomes of DNA-A and DNA-B were cloned in plasmid pUC18 at HindIII and XbaI sites, respectively. Partial tandem dimers of the viral clones were infective to Nicotiana benthamiana and tomato cv. Organ Spring through particle bombardment. Infected N. benthamiana plants exhibited downward and upward leaf curling, big veins, leaf puckering with interveinal chlorosis, and stunting. On tomato, symptoms were the same as those seen on naturally infected plants. Cloned DNA also infected Capsicum annuum cv. California Wonder (upward leaf curling and stunting) and tobacco cv. Xanthi (leaf curling and crinkling) but failed to infect Phaseolus vulgaris, okra, cotton, and N. glutinosa. The Varanasi isolate was sap transmissible (0.1 M potassium phosphate buffer, pH 7.0) from the bombarded plants to N. benthamiana and tomato cv. Organ Spring. DNA-A alone infected N. benthamiana (upward leaf curling and big veins) and tomato cv. Organ Spring (mild leaf curl), but symptoms were delayed and milder. Full-length genome sequencing revealed DNA-A (AY190290) contained 2,757 nt and DNA-B (AY190291) contained 2,688 nt. DNA-A of the Varanasi isolate shares 98.4% identity with a DNA-A sequence (AF449999) obtained from a tomato showing leaf curl symptoms from the same region and 97.1% identity with an isolate from Gujarat (900 km from Varanasi). All three sequences represent isolates of the same species, herein called Tomato leaf curl Gujarat virus, based on the priority of submission of the DNA sequence for the Gujarat region (ToLCGV; AF 413671). All isolates noted were obtained from GenBank. However, except for the DNA-A sequence, no other information is available for these ToLCGV isolates. DNA-A of the ToLCGV-Varanasi isolate shares 66.8% identity with Tomato leaf curl New Delhi virus, severe strain (ToLCNdV-Svr) (U15015), and 84.1% with Tomato leaf curl Karnataka virus (U38239). No DNA-B has been reported for these two ToLCGV isolates, and no infectious clone proving the etiology of the disease has been constructed, except for ToLCGV-Varanasi. DNA-B of ToLCGV-Varanasi shares 79.2% homology with ToLCNdV-Svr and 84.1% with ToLCNdV-Luc (X89653). These results suggest that the isolate from Varanasi belongs to ToLCGV, a previously undescribed geminivirus species causing a devastating tomato leaf curl disease in Gujarat and Uttar Pradesh. References: (1) H. C. Birnboim and J. Doly. Nucleic Acids Res. 7:1513, 1979. (2) K. M. Srivastava et al. J. Virol. Methods 51:297, 1995. (3) S. D. Wyatt and J. K. Brown. Phytopathology 86:1288, 1996.

Association of Tomato leaf curl New Delhi virus with mosaic and leaf curl disease of Chrysanthemum and its whitefly cryptic species

2020 ◽  
Vol 73 (3) ◽  
pp. 533-542 ◽  
Author(s):  
K. V. Ashwathappa ◽  
V. Venkataravanappa ◽  
C. N. Lakshminarayana Reddy ◽  
M. Krishna Reddy
Keyword(s):  
Cryptic Species ◽  
Tomato Leaf ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl
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