scholarly journals Proteomic Analysis ofBacillus thuringiensisStrain 4.0718 at Different Growth Phases

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Xiaohui Li ◽  
Xuezhi Ding ◽  
Liqiu Xia ◽  
Yunjun Sun ◽  
Can Yuan ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Growth Process ◽  
Growth Phases ◽  
Whole Cells ◽  
Crystal Proteins ◽  
Lysis Buffer ◽  
Sporulation Phase ◽  
Tof Ms ◽  
Insecticidal Crystal Proteins ◽  
Stable Formation

The growth process ofBacillus thuringiensisBt4.0718 strain was studied using proteomic technologies. The proteins of Bt whole cells at three phases—middle vegetative, early sporulation, and late sporulation—were extracted with lysis buffer, followed with separation by 2-DE and identified by MALDI-TOF/TOF MS. Bioactive factors such as insecticidal crystal proteins (ICPs) including Cry1Ac(3), Cry2Aa, and BTRX28, immune inhibitor (InhA), and InhA precursor were identified. InhA started to express at the middle vegetative phase, suggesting its contribution to the survival of Bt in the host body. At the early sporulation phase, ICPs started their expression. CotJC, OppA, ORF1, and SpoIVA related to the formation of crystals and spores were identified, the expression characteristics of which ensured the stable formation of crystals and spores. This study provides an important foundation for further exploration of the stable expression of ICPs, the smooth formation of crystals, and the construction of recombinant strains.

Membrane binding of insecticidal crystal proteins of Bacillus thuringiensis

2000 ◽  
Vol 28 (5) ◽  
pp. A438-A438
Author(s):  
Hiroshi Sakai ◽  
Tohru Komano ◽  
Masashi Yamagiwa
Keyword(s):  
Bacillus Thuringiensis ◽  
Membrane Binding ◽  
Crystal Proteins ◽  
Insecticidal Crystal Proteins

Larvicidal toxins fromBacillusthuringiensissubspp.kurstaki,morrisoni(straintenebrionis), andisraelensishave no microbicidal or microbiostatic activity against selected bacteria, fungi, and algae in vitro

2002 ◽  
Vol 48 (3) ◽  
pp. 262-267 ◽  
Author(s):  
J Koskella ◽  
G Stotzky
Keyword(s):  
Bacillus Thuringiensis ◽  
Mixed Culture ◽  
Gram Positive ◽  
Gram Negative ◽  
Crystal Proteins ◽  
Growth Of A Variety ◽  
Insecticidal Toxins ◽  
Antibiotic Effect ◽  
Insecticidal Crystal Proteins

The insecticidal toxins from Bacillus thuringiensis subspp. kurstaki (antilepidopteran), morrisoni strain tenebrionis (anticoleopteran), and israelensis (antidipteran) did not affect the growth of a variety of bacteria (8 gram-negative, 5 gram-positive, and a cyanobacterium), fungi (2 Zygomycetes, 1 Ascomycete, 2 Deuteromycetes, and 2 yeasts), and algae (primarily green and diatoms) in pure and mixed culture, as determined by dilution, disk-diffusion, and sporulation assays with purified free and clay-bound toxins. The insecticidal crystal proteins from B. thuringiensis subspp. kurstaki and israelensis had no antibiotic effect on various gram-positive bacteria.Key words: insecticidal toxins, Bacillus thuringiensis, microbiostatic, microbicidal.

scholarly journals Changes in Permeability of Brush Border Membrane Vesicles fromSpodoptera littoralis Midgut Induced by Insecticidal Crystal Proteins from Bacillus thuringiensis

1998 ◽  
Vol 64 (4) ◽  
pp. 1563-1565 ◽  
Author(s):  
B. Escriche ◽  
N. De Decker ◽  
J. Van Rie ◽  
S. Jansens ◽  
E. Van Kerkhove
Keyword(s):  
Bacillus Thuringiensis ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Osmotic Swelling ◽  
Midgut Cell ◽  
Crystal Proteins ◽  
Light Scattering Technique ◽  
Insecticidal Crystal Proteins

ABSTRACT Bacillus thuringiensis insecticidal crystal proteins (ICPs) are thought to induce pore formation in midgut cell membranes of susceptible insects. Cry1Ca, which is significantly active inSpodoptera littoralis, made brush border membrane vesicles permeable to KCl (osmotic swelling was monitored by the light scattering technique); the marginally active ICPs Cry1Aa, Cry1Ab, and Cry1Ac did not.

scholarly journals Diversity of Insecticidal Crystal proteins (ICPs) of indigenous Bacillus thuringiensis strains

2018 ◽  
Vol 5 ◽  
pp. 11-18
Author(s):  
Ganga G.C. ◽  
Charu Arjya ◽  
Yamuna Khadka ◽  
Sabina Dhamala
Keyword(s):  
Bacillus Thuringiensis ◽  
Slight Modification ◽  
Soil Samples ◽  
Colony Morphology ◽  
Selective Enrichment ◽  
Crystal Proteins ◽  
Selection Protocol ◽  
White Type ◽  
Insecticidal Crystal Proteins

Objectives: The purpose of this study was to characterize the indigenous Bacillus thuringiensis (Bt) isolated from the soil samples of central development region of Terai. Methods: A total of 50 soil samples collected from cultivated and barren fields of Terai region. Isolation was carried out using the acetate selection protocol as described by (Russell and Al 1987) with a slight modification. The Nutrient broth (NB) was acetated by using 0.25M sodium acetate which is a selective enrichment method for isolation of Bt. Characterization of the isolate was done by phenotyping methods (microscopy and biochemical). Results: No distinct variation was observed between the isolates of cultivable and uncultivable lands. Bt were categorized into7 different types based on colony morphology. The dominant colony was fried egg type identical with the reference strain, followed by flat white type of colony. The result showed that even though the colony morphology is same but the ICPs (Insecticidal crystal proteins) shapes produced by them vary, rod shapes (53.57%), spherical (10.71%), ovoid (8.3%), amorphous (17.85%), capheaded (9.5%). ICPs morphology reveal the cry1, cry2, cry3, cry4, cry8, cry 9, cry10 and cry11 types of gene may be present in the native isolates. Conclusion: This study represents the first report of several indigenous Bacillus thuringiensis strains with significantly different ICPs producing stains from hot tropical climate.

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