Anthocyanin Genes Involved in the Flower Coloration Mechanisms of Cymbidium kanran

The Orchidaceae, otherwise known as orchids, is one of the largest plant families and is renowned for its spectacular flowers and ecological adaptations. Various polymorphisms of orchid flower colour can attract pollinators and be recognised as valuable horticultural ornamentals. As one of the longest historic cultured orchids, Cymbidium kanran has been domesticated for more than 2,500 years and is an ideal species to study coloration mechanisms because of plentiful variations in floral coloration and abundant traditional varieties. In this study, we used two distinct colour-type flowers of C. kanran as experimental materials to elucidate the mechanism of flower coloration. High-performance liquid chromatography (HPLC) analysis revealed that anthocyanins in purple-red-type flowers include three types of anthocyanidin aglycones, peonidin, malvidin, and cyanidin, whereas anthocyanins are lacking in white-type flowers. Through comparative transcriptome sequencing, 102 candidate genes were identified as putative homologues of colour-related genes. Based on comprehensive correlation analysis between colour-related compounds and gene expression profiles, four candidates from 102 captured genes showed a positive correlation with anthocyanidin biosynthesis. Furthermore, transient expression of CkCHS-1, CkDFR, and CkANS by particle bombardment confirmed that recovery of their expression completed the anthocyanin pathway and produced anthocyanin compounds in white-type flowers. Collectively, this study provided a comprehensive transcriptomic dataset for Cymbidium, which significantly facilitate our understanding of the molecular mechanisms of regulating floral pigment accumulation in orchids.

Content of nitrates and nitrites in unprocessed raw beef

The aim of the study was to examine the contents of nitrates and nitrites in raw beef coming from cattle of the Polish Holstein-Friesian breed, black-and-white type, originating from different parts of Poland. The research material comprised semitendinosus and longissimus thoracis muscles obtained from 176 heads of cattle. The content of nitrites in the examined muscles ranged from 0.6 to 13.3 mg kg^–1 in rump muscles and from 0.8 to 13.7 mg kg^–1 in muscles of the back. The most samples were characterised by the presence of nitrites in the range from 1 to 5 mg kg^–1, and the least samples from 11 to 14 mg kg^–1. The contents of nitrates were significantly higher, ranging from 10.2 to 73.5 mg kg^–1in the semitendinosus muscle and from 10.4 to 74.3 mg kg^–1in the thoracis longissimus muscle. Such and higher level of contamination may be the cause of meat discolouration after heat treatment despite the absence of curing ingredients. It is necessary to continuously monitor the concentration of nitrates in raw materials, water and feed to ensure the complete safety of food of animal origin.

An Intuitive Introduction to Fractional and Rough Volatilities

Here, we review some results of fractional volatility models, where the volatility is driven by fractional Brownian motion (fBm). In these models, the future average volatility is not a process adapted to the underlying filtration, and fBm is not a semimartingale in general. So, we cannot use the classical Itô’s calculus to explain how the memory properties of fBm allow us to describe some empirical findings of the implied volatility surface through Hull and White type formulas. Thus, Malliavin calculus provides a natural approach to deal with the implied volatility without assuming any particular structure of the volatility. The aim of this paper is to provides the basic tools of Malliavin calculus for the study of fractional volatility models. That is, we explain how the long and short memory of fBm improves the description of the implied volatility. In particular, we consider in detail a model that combines the long and short memory properties of fBm as an example of the approach introduced in this paper. The theoretical results are tested with numerical experiments.

Using Bioelements Isotope Ratios and Fatty Acid Composition to Deduce Beef Origin and Zebu Feeding Regime in Cameroon

The purpose of this study was to address the lack of knowledge regarding the stable isotopic composition of beef from zebu cattle reared in tropical Africa. Sixty beef carcasses belonging to the most common zebu breeds (Goudali, white Fulani, and red Mbororo) were selected and classified according to their subcutaneous fat color (white, cream or yellow). The stable isotope ratios of five bioelements—H, O, C, N, and S—in muscle fractions and the fatty acids composition were analyzed. Zebu meat from Cameroon shows peculiar δ13C values, related to the almost exclusive intake of grazed tropical grasses with photosynthetic cycle C4. It also shows δ2H and δ18O values higher than those reported in other areas of the world and correlated with the isotopic composition of animal drinking water. The white subcutaneous fat (“white type”) zebu showed higher δ2H and lower δ13C than the “yellow type”, that is correlated with a higher content of polyunsaturated fatty acid (PUFA) and a lower amount of saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA). Multielement analysis seems to provide promising results for tracing the regional origin of Cameroon beef and some aspects of the livestock system, such as the nutritional status of the animals.

Discourse, Figure, d’après Lyotard.

The presence of the written element in cinema goes back to the early silent movies era, to express meanings that were not enough comprehensible just through images. The use of text charts as means to support and to implement narrative almost invariable consisted of black cards with centered white type (rarely the opposite, i.e., black type on white boards), occasionally utilizing graphic features as ornaments.These letterings inserted between scenes, either before or after to which they referred, sometimes had a deranged narrative effect because of interrupting the action flow. But words, when added to the cinematographic image, can indeed communicate certain abstract concepts such as date time lapse, local; evince characters speeches; describe some action not performed in the movie.This paper aims to investigate the change of status of the written element as an accessory apparatus to a central and structural element of the movie, specifically in the experimental and avant-garde cinema, considering Marcel Duchamp’s Anémic Cinéma (1926) its inaugural example.The incorporation of textual elements can be understood within the very process of the visual arts in the first decades of the twentieth century since Braque’s Gueridon (1913) and Picasso’s Bottle of Vieux Marc, Glass, Guitar and Newspaper (1913) through the Cubist and the Dada conceptual and formal strategies.The Conceptual art of the sixties and seventies permeates expressions of film experimentalism that will be analyzed for its use of text condition, where Michael Snow’s So this is, already in early 80ies (1982) is to be highlighted.

De-Noising of Corrupted Fluoroscopy Images Based on a New Multi-Line Algorithm

Fluoroscopic images are a field of medical images that depends on the quality of image for correct diagnosis; the main trouble is the de-nosing and how to keep the poise between degradation of noisy image, from one side, and edge and fine details preservation, from the other side, especially when fluoroscopic images contain black and white type noise with high density. The previous filters could usually handle low/medium black and white type noise densities, that expense edge, =fine details preservation and fail with high density of noise that corrupts the images. Therefore, this paper proposed a new Multi-Line algorithm that deals with high-corrupted image with high density of black and white type noise. The experiments achieved images with a high quality and effectively preserved edge and fine details against black and white type noise densities depending on Peak Signal to Noise Ratio (PSNR), Mean Squared Error (MSE), and Image Enhancement Factor (IEF) measures.

Pengaruh Cangkang Keong Sawah (Pila ampullacea) Terhadap Pembentukan Tulang Baru (Remodeling Tulang)

Rice fields conch is believed by people as an alternative medicine that capable to treat many kind of diseases, such as diabetes, jaundice, liver, ulcer, cholesterol, and preventing osteoporosis, more in-depth scientific studies regarding the efficacy of rice field conch for human health are still not widely done. All of this statement are still empirical data from the experiences of rice field conch users, so it needs further research. The purpose of this research was to determine whether the content of the rice field conch shell (Pila ampullacea) can affect the bone remodeling process. Types of research that has been used was laboratory experiment with control group post test only design. The population of this research used white type of rat Galur Wistar (Rattus norvegicus L.), with the minimum number of samples needed are 4 rats from each group. So total of the samples are 20 white rats Galur Wistar (Rattusnorvegicus L.). Research conducted at the Pharmacology Laboratory of Andalas University, Anatomical pathology Laboratory Siti rahmah islamic hospital and Anatomical Pathology Laboratory Andalas University using Oneway ANOVA test. The results of the research obtained that the average number of osteoblasts in the control group (-) with the lowest average number of osteoblasts was 14.3 per five fields of view. The highest increase in average occurred in the administration of Pila ampullacea 100 mg, which is 36.6 per five fields of view. The average number of osteoclasts in the control group (-) was also seen to be the lowest at 1.55 per five fields of view and the increase of osteoclasts average was highest in the administration of 100 mg of ampullacea, which was 3.25 per five fields of view and it was shown that the supply of rice field conch shell powder (Pila ampullacea) could affect bone remodeling processes with p <0.05

Intraspecific plasticity in circadian rhythms within Euglena gracilis strain Z

Natural plasticity in overt circadian rhythms can be observed in various animals. Little is known about how this phenomenon help Euglena gracilis adapt to environmental stimuli. We used four groups of strain Z. Two groups were from our laboratory, ZObihiro1 and ZObihiro2; Third group was from the National Institute for Environmental Studies, Japan (ZNIES-48) and the other was from Osaka Prefecture University (ZOsaka). The latter two were grown photoautotrophically at a light intensity of 84 μmol m-2 s-1 (day-white type lamps) at 25°C with air bubbling, as were ours, for two months prior to experiments. Results showed that ZObihiro2 and ZOsaka grew faster than ZObihiro1 and ZNIES-48. Upon transferring from light to darkness, population growth ceased within 8-10 h with the cell number increase in the dark of 41% in ZObihiro1 and ZObihiro2, 35% in ZOsaka and remarkably low 22% in ZNIES-48. Timing of cell division bursts in the circadian rhythm of cell population growth in 24 h light-dark cycles was the same in all four groups. Magnitudes of the rhythm were different: both ZObihiro1 and ZObihiro2 completely doubled, but ZNIES-48 multiplied by 1.9, and ZOsaka multiplied feebly by 1.7. The photoinduction of commitment to cell division in DD followed a circadian rhythm. All four showed the same peak at subjective dusk, but the amplitudes differed in the order, ZObihiro2 > ZOsaka > ZObihiro1 >> ZNIES-48. The resistance to photosensitization against Rose-Bengal follows a clear circadian rhythm in all substrains except in ZNIES-48. ZObihiro1 and ZOsaka showed the phasing similar to UV resistance rhythm, but ZObihiro2 did not. These results suggest the plasticity of circadian rhythms within a species, if not within a strain. Moreover, it is also apparent that different substrains/ecotypes present within the same Z strain. Int. J. Appl. Sci. Biotechnol. Vol 7(2): 207-216

Growth performance of white, black and bronze color heritage turkeys under semi-intensive system

The present study was conducted to compare the growth performance of White, Black and Bronze color type of turkeys, which have been currently introduced in Bangladesh. The birds were reared under semi-intensive system at BAU Poultry Farm with supplementation of commercial broiler grower and layer feeds. Key objectives were to investigate the growth performance of locally found turkeys (known as heritage turkeys) under semi-intensive rearing system and to recommend turkey as a supplementary part of existing poultry business in the country. A total of 46 poults of White, Black and Bronze color type unsexed birds of 5 weeks age were housed. Birds were reared under similar management conditions and feeds were purchased from local market. No significant difference (p>0.05) in live weight for three color types were found. At the end of 21 weeks rearing, Bronze type turkeys attained highest live body weight (3720.71g/bird) while White type turkeys attained lowest (3282.29g/bird). The Black type turkeys however, attained 3552.86g/bird body weight. The live weight gain was increased gradually but decreased again after 18 weeks of age. Weekly weight gains were highest in all color types at 18 weeks of age where white type male turkey attained the highest (440.71g/bird) and black type showed the lowest weight gain (345.00g/bird). No significant difference (p>0.05) in FCR among the three color types were found. Survivability under semi intensive system of three different color types, White, Black and Bronze were 100%, 88.46% and 91.67% respectively. Taken together, the growth performance of Bronze type turkey was superior to other color types used in this experiment. Therefore, it can be concluded that turkey farming will viable in Bangladesh with available local varieties under semi-intensive system through feeding of commercial broiler and layer feeds. J. Bangladesh Agril. Univ. 16(3): 471–477, December 2018