scholarly journals Comparative Study of VariousE. coliStrains for Biohydrogen Production Applying Response Surface Methodology

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Péter Bakonyi ◽  
Nándor Nemestóthy ◽  
Katalin Bélafi-Bakó

The proper strategy to establish efficient hydrogen-producing biosystems is the biochemical, physiological characterization of hydrogen-producing microbes followed by metabolic engineering in order to give extraordinary properties to the strains and, finally, bioprocess optimization to realize enhanced hydrogen fermentation capability. In present paper, it was aimed to show the utility both of strain engineering and process optimization through a comparative study of wild-type and genetically modifiedE. colistrains, where the effect of two major operational factors (substrate concentration and pH) on bioH2production was investigated by experimental design and response surface methodology (RSM) was used to determine the suitable conditions in order to obtain maximum yields. The results revealed that by employing the genetically engineeredE. coli(DJT 135) strain under optimized conditions (pH: 6.5; Formate conc.: 1.25 g/L), 0.63 mol H2/mol formate could be attained, which was 1.5 times higher compared to the wild-typeE. coli(XL1-BLUE) that produced 0.42 mol H2/mol formate (pH: 6.4; Formate conc.: 1.3 g/L).

2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.


2012 ◽  
Vol 581-582 ◽  
pp. 819-822 ◽  
Author(s):  
Bin Meng ◽  
Jin Hui Peng

The corundum-mullite was toughened by in-situ synthesized mullite whiskers and the process parameters influencing the fracture toughness of corundum-mullite, such as sintering temperature, addition amount of AlF3 and V2O5, were optimized by means of response surface method. Corundum-mullite with fracture toughness of 9.44 MPa.m-1/2 could be obtained under the optimized conditions, i.e. sintering temperature of 1400°C, 4.8 wt.% of AlF3 and 5.8 wt.% of V2O5. The results showed that it was feasible to prepare corundum-mullite toughened by in-situ synthesized mullite whiskers by the optimized parameters. In addition, an accurate model based on response surface method was proposed to predict the experimental results.


2018 ◽  
Vol 5 (9) ◽  
pp. 18089-18094 ◽  
Author(s):  
Vinay Kumar ◽  
Amit Kumar ◽  
Sanjay Kumar ◽  
N.K. Singh

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