scholarly journals Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions

2020 ◽  
Author(s):  
Ko Tsutsui ◽  
Hiroki Machida ◽  
Ritsuko Morita ◽  
Asako Nakagawa ◽  
Kiyotoshi Sekiguchi ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Cellular Origin ◽  
Molecular Identity ◽  
Tissue Interactions ◽  
Structural Specialization ◽  
Extracellular Matrix Molecules ◽  
Structural Details ◽  
Tissue Interfaces

AbstractInter-tissue interaction is fundamental to multicellularity. Although the basement membrane (BM) is located at tissue interfaces, its mode of action in inter-tissue interactions remains poorly understood, mainly because the molecular and structural details of the BM at distinct inter-tissue interfaces remain unclear. By combining quantitative transcriptomics and immunohistochemistry, we systematically identify the cellular origin, molecular identity and tissue distribution of extracellular matrix molecules in mouse hair follicles, and reveal that BM composition and architecture are exquisitely specialized for distinct inter-tissue interactions, including epidermal–fibroblast, epidermal–muscle and epidermal–nerve interactions. The epidermal–fibroblast interface, namely, hair germ–dermal papilla interface, makes asymmetrically organized side-specific heterogeneity in BM, defined by the newly characterized interface, hook and mesh BMs. One component of these BMs, laminin α5, is required for the topological and functional integrity of hair germ–dermal papilla interactions. Our study highlights the significance of BM heterogeneity in distinct inter-tissue interactions.

scholarly journals Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ko Tsutsui ◽  
Hiroki Machida ◽  
Asako Nakagawa ◽  
Kyungmin Ahn ◽  
Ritsuko Morita ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Cellular Origin ◽  
Tissue Interactions ◽  
Structural Specialization ◽  
Extracellular Matrix Molecules ◽  
Structural Details ◽  
Cycle Regulation ◽  
Tissue Interfaces

AbstractInter-tissue interaction is fundamental to multicellularity. Although the basement membrane (BM) is located at tissue interfaces, its mode of action in inter-tissue interactions remains poorly understood, mainly because the molecular and structural details of the BM at distinct inter-tissue interfaces remain unclear. By combining quantitative transcriptomics and immunohistochemistry, we systematically identify the cellular origin, molecular identity and tissue distribution of extracellular matrix molecules in mouse hair follicles, and reveal that BM composition and architecture are exquisitely specialized for distinct inter-tissue interactions, including epithelial–fibroblast, epithelial–muscle and epithelial–nerve interactions. The epithelial–fibroblast interface, namely, hair germ–dermal papilla interface, makes asymmetrically organized side-specific heterogeneity in the BM, defined by the newly characterized interface, hook and mesh BMs. One component of these BMs, laminin α5, is required for hair cycle regulation and hair germ–dermal papilla anchoring. Our study highlights the significance of BM heterogeneity in distinct inter-tissue interactions.

Morphological changes at the basement membrane during some tissue interactions in the integument

1983 ◽  
Vol 61 (8) ◽  
pp. 957-966 ◽  
Author(s):  
M. H. Hardy ◽  
E. A. Goldberg
Keyword(s):  
Basement Membrane ◽  
Morphological Changes ◽  
Dermal Papilla ◽  
Enzyme Treatment ◽  
Follicle Development ◽  
Lamina Densa ◽  
Tissue Interactions ◽  
Follicle Differentiation

Changes at the basement membrane have been reported at the time of tissue interactions during the development of a number of integumentary organs. During the development of vibrissa follicles of the mouse in vitro, gaps appeared in the lamina densa surrounding the dermal papilla at the time of the second, specific dermal message for follicle differentiation. Direct contacts between epithelial and mesenchymal cells were made through these gaps. This area has now been studied in vivo over the same period of follicle development. In addition, the epithelial part of the follicle has been separated from the dermal papilla by (i) enzyme treatment which destroys the lamina densa and (ii) chelating agents which leave the lamina densa attached to the dermal papilla. The resulting surfaces of epithelium and dermal papilla were examined by scanning and transmission electron microscopy. Small gaps first appeared in the lamina densa at the same substage of follicle development as in vitro and were present at the next substage. Processes from the dermal papilla mesenchyme cells traversed these gaps and made close contacts with the epithelial cells just prior to their differentiation into inner root sheath and hair. Instructive interaction through cell surfaces is suggested.

The Ultrastructure of the Dermal Papilla-Epithelial Junction in Normal and Alopecia Areata Hair Follicles

2006 ◽  
Vol 642 (1) ◽  
pp. 476-477
Author(s):  
M. NUTBROWN ◽  
S. MACDONALD HULL ◽  
M. J. THORNTON ◽  
W. J. CUNLIFFE ◽  
V. A. RANDALL
Keyword(s):  
Alopecia Areata ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Epithelial Junction

Hormones and Hair Growth: Variations in Androgen Receptor Content of Dermal papilla Cells Cultured from Human and Red Deer (Cervus Elaphus) Hair Follicles.

1993 ◽  
Vol 101 (s1) ◽  
pp. 114S-120S ◽  
Author(s):  
Valerie Anne Randall ◽  
Margaret Julie Thornton ◽  
Andrew Guy Messenger ◽  
Nigel Andrew Hibberts ◽  
Andrew Stewart Irving Loudon ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Cervus Elaphus ◽  
Hair Growth ◽  
Red Deer ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Cells Cultured

Smooth muscle alpha-actin is a marker for hair follicle dermis in vivo and in vitro

1991 ◽  
Vol 99 (3) ◽  
pp. 627-636 ◽  
Author(s):  
C.A. Jahoda ◽  
A.J. Reynolds ◽  
C. Chaponnier ◽  
J.C. Forester ◽  
G. Gabbiani
Keyword(s):  
Smooth Muscle ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Smooth Muscle Alpha Actin ◽  
Actin Expression ◽  
Alpha Actin ◽  
Sheath Cells

We have examined the expression of smooth muscle alpha-actin in hair follicles in situ, and in hair follicle dermal cells in culture by means of immunohistochemistry. Smooth muscle alpha-actin was present in the dermal sheath component of rat vibrissa, rat pelage and human follicles. Dermal papilla cells within all types of follicles did not express the antigen. However, in culture a large percentage of both hair dermal papilla and dermal sheath cells were stained by this antibody. The same cells were negative when tested with an antibody to desmin. Overall, explant-derived skin fibroblasts had relatively low numbers of positively marked cells, but those from skin regions of high hair-follicle density displayed more smooth muscle alpha-actin expression than fibroblasts from areas with fewer follicles. 2-D SDS-PAGE confirmed that, unlike fibroblasts, cultured papilla cells contained significant quantities of the alpha-actin isoform. The rapid switching on of smooth muscle alpha-actin expression by dermal papilla cells in early culture, contrasts with the behaviour of smooth muscle cells in vitro, and has implications for control of expression of the antigen in normal adult systems. The very high percentage of positively marked cultured papilla and sheath cells also provides a novel marker of cells from follicle dermis, and reinforces the idea that they represent a specialized cell population, contributing to the heterogeneity of fibroblast cell types in the skin dermis, and possibly acting as a source of myofibroblasts during wound healing.

Histochemical Differentiation of the Basement Membrane of the Mouse Seminiferous Tubule

1962 ◽  
Vol s3-103 (63) ◽  
pp. 385-391
Author(s):  
A. H. BAILLIE
Keyword(s):  
Basement Membrane ◽  
Protein Metabolism ◽  
Seminiferous Tubule ◽  
Glucuronic Acid ◽  
Chondroitin Sulphate ◽  
Hair Follicles ◽  
Ground Substance ◽  
Seminiferous Tubules ◽  
Active Protein ◽  
Pas Reaction

The ground substance of the testis of the albino mouse is PAS-positive but not metachromatic, and probably highly aggregated. The basement of the seminiferous tubules is intensely PAS-positive, metachromatic, and possibly not so highly aggregated. The reactivity of the ground substance to the PAS reaction and toluidine blue is tentatively ascribed to the presence of chondroitin sulphate C: this compound, previously known to contain N acetyl-galactosamine, glucuronic acid, tyrosine and tryptophane, is associated with arginine. The genesis of the basement membrane of the seminiferous tubule is shown to include the formation of a sheath of atypical elongated fibroblasts, the secretion of a PAS positive, metachromatic substance associated with arginine between this sheath and the seminiferous tubule, the appearance of mitochondria in the cells of the sheath, and lastly, the acquisition of alkaline phosphatase by these fibroblasts and its spread to the intervening ground substance. These changes are thought to be related to the structural and nutritional requirements of the seminiferous tubules. In its intense positive reaction to PAS and in its metachromasy, the basement membrane of the seminiferous tubule agrees with the ground substance adjacent to sites of active protein metabolism, such as growing tumours, embryonic organs, hair follicles, and skin.

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