scholarly journals Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

2020 ◽  
Author(s):  
Hammad A. Faizi ◽  
Cody J. Reeves ◽  
Vasil N. Georgiev ◽  
Petia M. Vlahovska ◽  
Rumiana Dimova
Keyword(s):  
Confocal Microscopy ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Bending Rigidity ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Bilayer Membranes ◽  
Bending Modulus ◽  
Contrast Microscopy ◽  
Thermally Driven

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

scholarly journals Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

Soft Matter ◽  
2020 ◽  
Vol 16 (39) ◽  
pp. 8996-9001
Author(s):  
Hammad A. Faizi ◽  
Cody J. Reeves ◽  
Vasil N. Georgiev ◽  
Petia M. Vlahovska ◽  
Rumiana Dimova
Keyword(s):  
Phase Contrast ◽  
Imaging Technique ◽  
Phase Contrast Imaging ◽  
Phase Contrast Microscopy ◽  
Bending Rigidity ◽  
Giant Unilamellar Vesicles ◽  
Contrast Imaging ◽  
Unilamellar Vesicles ◽  
Contrast Microscopy

Bending rigidity measured with fluctuation spectroscopy is insensitive to the imaging technique: do’s and do not’s when using confocal and phase contrast imaging.

Dynamic behaviour of giant unilamellar vesicles induced by the uptake of [70]fullerene

2014 ◽  
Vol 50 (11) ◽  
pp. 1288-1291 ◽  
Author(s):  
Atsushi Ikeda ◽  
Tomohiro Hida ◽  
Tatsuya Iizuka ◽  
Manami Tsukamoto ◽  
Jun-ichi Kikuchi ◽  
...  
Keyword(s):  
Exchange Reaction ◽  
Lipid Membranes ◽  
Phase Contrast ◽  
Dynamic Behaviour ◽  
Phase Contrast Microscopy ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Contrast Microscopy

We have directly observed the processes involved in a C70-exchange reaction from γ-cyclodextrin cavities to lipid membranes using GUVs by phase contrast microscopy.

scholarly journals Rough Dental Implant Surfaces and Peri-Implantitis: Role of Phase-Contrast Microscopy, Laser Protocols, and Modified Home Oral Hygiene in Maintenance. A 10-Year Retrospective Study

2021 ◽  
Vol 11 (11) ◽  
pp. 4985
Author(s):  
Gianluigi Caccianiga ◽  
Gérard Rey ◽  
Paolo Caccianiga ◽  
Alessandro Leonida ◽  
Marco Baldoni ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Phase Contrast ◽  
Vertical Movement ◽  
Subgingival Plaque ◽  
Phase Contrast Microscopy ◽  
Implant Surface ◽  
Total Percentage ◽  
Contrast Microscopy

The aim of this study was to evaluate two different kinds of rough implant surface and to assess their tendency to peri-implantitis disease, with a follow-up of more than 10 years. Data were obtained from a cluster of 500 implants with Ti-Unite surface and 1000 implants with Ossean surface, with a minimum follow-up of 10 years. Implants had been inserted both in pristine bone and regenerated bone. We registered incidence of peri-implantitis and other causes of implant loss. All patients agreed with the following maintenance protocol: sonic brush with vertical movement (Broxo), interdental brushes, and oral irrigators (Broxo) at least two times every day. For all patients with implants, we evaluated subgingival plaque samples by phase-contrast microscopy every 4 months for a period of more than 10-years. Ti-Unite surface implants underwent peri-implantitis in 1.6% of the total number of implants inserted and Ossean surface implants showed peri-implantitis in 1.5% of the total number of implants. The total percentage of implant lost was 4% for Ti-Unite surfaces and 3.6% for Ossean surfaces. Strict control of implants leads to low percentage of peri-implantitis even for rough surfaces dental implants.

scholarly journals Coherent noise reduction in digital holographic phase contrast microscopy by slightly shifting object

2011 ◽  
Vol 19 (5) ◽  
pp. 3862 ◽  
Author(s):  
Feng Pan ◽  
Wen Xiao ◽  
Shuo Liu ◽  
FanJing Wang ◽  
Lu Rong ◽  
...  
Keyword(s):  
Noise Reduction ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Coherent Noise ◽  
Contrast Microscopy

In vitro Maturation of the Exoerythrocytic Stage of Plasmodium knowlesi Observed under Phase Contrast Microscopy

1990 ◽  
Vol 76 (6) ◽  
pp. 923 ◽  
Author(s):  
Pascal Millet ◽  
William E. Collins ◽  
Claude E. Monken ◽  
Bobby G. Brown
Keyword(s):  
Phase Contrast ◽  
In Vitro Maturation ◽  
Plasmodium Knowlesi ◽  
Phase Contrast Microscopy ◽  
Contrast Microscopy
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