Rough Dental Implant Surfaces and Peri-Implantitis: Role of Phase-Contrast Microscopy, Laser Protocols, and Modified Home Oral Hygiene in Maintenance. A 10-Year Retrospective Study

The aim of this study was to evaluate two different kinds of rough implant surface and to assess their tendency to peri-implantitis disease, with a follow-up of more than 10 years. Data were obtained from a cluster of 500 implants with Ti-Unite surface and 1000 implants with Ossean surface, with a minimum follow-up of 10 years. Implants had been inserted both in pristine bone and regenerated bone. We registered incidence of peri-implantitis and other causes of implant loss. All patients agreed with the following maintenance protocol: sonic brush with vertical movement (Broxo), interdental brushes, and oral irrigators (Broxo) at least two times every day. For all patients with implants, we evaluated subgingival plaque samples by phase-contrast microscopy every 4 months for a period of more than 10-years. Ti-Unite surface implants underwent peri-implantitis in 1.6% of the total number of implants inserted and Ossean surface implants showed peri-implantitis in 1.5% of the total number of implants. The total percentage of implant lost was 4% for Ti-Unite surfaces and 3.6% for Ossean surfaces. Strict control of implants leads to low percentage of peri-implantitis even for rough surfaces dental implants.

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Phase contrast microscopy of microbial aggregates in the gingival sulcus of Macaca mulatta. Subgingival plaque bacteria in Macaca mulatta

Journal Of Clinical Periodontology ◽

10.1111/j.1600-051x.1983.tb01290.x ◽

1983 ◽

Vol 10 (4) ◽

pp. 412-421 ◽

Cited By ~ 6

Author(s):

Penelope J. Leggott ◽

A. W. Anderson ◽

I. Punwani ◽

T. Sabet ◽

R. Murphy ◽

...

Keyword(s):

Macaca Mulatta ◽

Phase Contrast ◽

Subgingival Plaque ◽

Phase Contrast Microscopy ◽

Contrast Microscopy

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On the Mechanism of Primary Hemostasis

10.1055/s-0039-1687525 ◽

1979 ◽

Author(s):

H.K. Breddin ◽

N. Bender ◽

K. Kirchmaier ◽

M. Ziemen

Keyword(s):

Phase Contrast ◽

Thrombus Formation ◽

Vascular Lesion ◽

Phase Contrast Microscopy ◽

New Techniques ◽

Primary Hemostasis ◽

Contrast Microscopy ◽

Subcutaneous Tissues ◽

Citrate Blood

The sticking of platelets to basal membranes and to collagen fibers followed by subsequent aggregation are thought to be the first steps in hemostasis. During the first seconds after a vascular lesion platelets are stimulated outside the vessel and are transformed by forming pseudopodes and by sphering. Subcutaneous tissues of different species [human, Pig, dog, rat) as well as other tissues contain a lipoprotein with low tnrombo-plastic activity that stimulates platelets very rapidly. The action of this hemostasis activating factor (HaF) can be evaluated in fresh citrate blood by phase contrast microscopy and less specifically by the enhancement of platelet retention and the acceleration but not induction of platelet aggregation.HaF may play an important part in the activation of primary hemostasis. The proteins defective in v.-Willebrand’s syndrome are probably catalysing the stimulating effect of HaF on platelets. With new techniques the mechanism of the first steps in hemostasis can be further elucidated as well as the role of these mechanisms in thrombus formation.

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Phase Contrast Morphology of Coagulation Clot Maturation and Fibrinolysis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656286 ◽

1965 ◽

Vol 13 (01) ◽

pp. 047-059 ◽

Cited By ~ 1

Author(s):

J. R Jannach

Keyword(s):

Refractive Index ◽

Phase Contrast ◽

Positive Phase ◽

Phase Contrast Microscopy ◽

Clot Retraction ◽

Fibrin Formation ◽

Contrast Microscopy

SummaryMorphologic observations of coagulation, clot maturation and fibrinolysis in thin standardized clots were presented using negative and positive phase contrast microscopy. The study described the beginning of fibrin formation in centers away from platelets and platelet clumps but usually associated with platelet fragments. Although the preparations failed to reveal clot retraction, the fibrin strands did show a change in refractive index. Intact clot preparations were demonstrated several weeks after incubation at 37° 0.The observation of greatest significance, in the light of recent studies accentuating the role of platelets in hemostasis, was the lysis of platelets and platelet clumps which had undergone viscous metamorphosis. This action of plasmin on platelets may be of great importance in the prevention of hemostasis in the fibrinolytic syndrome and the destruction of damaged platelets in the normal circulation.

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