scholarly journals Three-dimensional Multi-site Random Access Photostimulation (3D-MAP)

2020 ◽  
Author(s):  
Yi Xue ◽  
Laura Waller ◽  
Hillel Adesnik ◽  
Nicolas Pégard
Keyword(s):  
High Precision ◽  
Three Dimensional ◽  
Random Access ◽  
Network Control ◽  
Structured Illumination ◽  
Wireless Devices ◽  
Neural Ensemble ◽  
Neuroscience Research ◽  
Intact Brain ◽  
Optogenetic Control

AbstractHigh precision optical control of neural ensemble activity is essential for understanding brain function and may ultimately revolutionize the treatment of brain disease. Currently only multiphoton holographic optogenetics achieves the requisite spatial resolution for precise neural network control. However, it suffers from critical drawbacks that limit the number of addressable neurons and prevent the development of implantable or wireless devices. Therefore, achieving high-resolution optogenetic control with one-photon activation is essential to catalyze a dramatic leap in spatiotemporally precise multi-site optogenetic technology for research and clinical use. To overcome this challenge, we developed a new light sculpting technique that can synthesize custom illumination patterns in 3D by rapidly projecting structured illumination patterns along with novel computational methods for precise optogenetic control, termed ‘3D-MAP’, for Three-dimensional Multi-site random Access Photostimulation. This technology enables the optogenetic synthesis of complex spatiotemporal sequences of neural activity in the intact brain. As a one-photon photostimulation technology, 3D-MAP can be widely adopted for custom optogenetic applications by the neuroscience research community, and opens the door to scalable, wireless high precision optical brain interfaces.

scholarly journals Three-dimensional Multi-site Random Access Photostimulation (3D-MAP)

2020 ◽  
Author(s):  
Yi Xue ◽  
Laura Waller ◽  
Hillel Adesnik ◽  
Nicolas Pégard
Keyword(s):  
Large Volume ◽  
Three Dimensional ◽  
Random Access ◽  
Neural Ensemble ◽  
Volume State ◽  
Large Numbers ◽  
Electrophysiological Measurements ◽  
Intact Brain

Abstract Optical control of neural ensemble activity has been crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at extremely fast rates over a large volume. State-of-the-art multiphoton holographic optogenetics requires high power illumination that only address relatively small populations of neurons in parallel. Conversely, one-photon holographic techniques can stimulate more neurons but with a trade-off between resolution and addressable volume. We introduce a new one-photon light sculpting technique, termed Three-Dimensional Multi-site random Access Photostimulation (3D-MAP), that simultaneously overcomes all these limitations by dynamically modulating light in both the spatial and angular domain at multi-kHz rates. Electrophysiological measurements confirm that 3D-MAP achieves high spatial precision in vitro and in vivo. Using 3D-MAP, we then interrogate neural circuits with 3D multi-site illumination with high resolution over a large volume of intact brain that existing techniques cannot achieve.

Three-dimensional auto-stereoscopic animated image with a long viewing distance using high-precision image correction

2009 ◽  
Author(s):  
Takehito Teraguchi ◽  
Hiromasa Yamashita ◽  
Ken Masamune ◽  
Takeyoshi Dohi ◽  
Hongen Liao
Keyword(s):  
High Precision ◽  
Three Dimensional ◽  
Viewing Distance ◽  
Image Correction

scholarly journals Parallel, linear, and subnanometric 3D tracking of microparticles with Stereo Darkfield Interferometry

2021 ◽  
Vol 7 (6) ◽  
pp. eabe3902
Author(s):  
Martin Rieu ◽  
Thibault Vieille ◽  
Gaël Radou ◽  
Raphaël Jeanneret ◽  
Nadia Ruiz-Gutierrez ◽  
...  
Keyword(s):  
High Precision ◽  
High Throughput ◽  
Single Molecule ◽  
Particle Tracking ◽  
Focal Plane ◽  
Tracking System ◽  
Three Dimensional ◽  
3D Tracking ◽  
Single Molecule Force Spectroscopy ◽  
A New Technique

While crucial for force spectroscopists and microbiologists, three-dimensional (3D) particle tracking suffers from either poor precision, complex calibration, or the need of expensive hardware, preventing its massive adoption. We introduce a new technique, based on a simple piece of cardboard inserted in the objective focal plane, that enables simple 3D tracking of dilute microparticles while offering subnanometer frame-to-frame precision in all directions. Its linearity alleviates calibration procedures, while the interferometric pattern enhances precision. We illustrate its utility in single-molecule force spectroscopy and single-algae motility analysis. As with any technique based on back focal plane engineering, it may be directly embedded in a commercial objective, providing a means to convert any preexisting optical setup in a 3D tracking system. Thanks to its precision, its simplicity, and its versatility, we envision that the technique has the potential to enhance the spreading of high-precision and high-throughput 3D tracking.

Absolute Measurement of Aspheric Surfaces Using White-Light Interferometry

2007 ◽  
Vol 364-366 ◽  
pp. 80-85
Author(s):  
Su Ping Chang ◽  
Tie Bang Xie ◽  
Xuang Ze Wang ◽  
Jun Guo
Keyword(s):  
High Precision ◽  
White Light ◽  
Spherical Surface ◽  
Three Dimensional ◽  
Influence Factors ◽  
Horizontal Displacement ◽  
Absolute Measurement ◽  
Aspheric Surfaces ◽  
Interferometric Technique ◽  
Main Influence

White-light interferometric technique has been widely applied in the measurement of three-dimensional profiles and roughness with high-precision. Based on the characteristic of interferometric technique, a new method combined with image location and a three-dimensional stage is proposed to achieve the non-contact absolute shape measurement for aspheric and spherical surface in a slarge range. The interference fringes vary with the horizontal displacement of the measured surface, the surface information was obtained by locating the transformation of the maximal intensity in the interferograms. Two main influence factors are discussed; they are performance of the inerferimetric microscope and the stage. Since the performance of the stage directly determines the measurement precision, a three-dimensional displacement stage with a large range and a high precision was developed. Some experiments were carried out to verify the performance of the three-dimensional displacement stage and the validity of the new measurement method with satisfactory results.

scholarly journals 3D DNA structural barcode copying and random access

2020 ◽  
Author(s):  
Filip Bošković ◽  
Alexander Ohmann ◽  
Ulrich F. Keyser ◽  
Kaikai Chen
Keyword(s):  
Data Storage ◽  
Single Molecule ◽  
Self Assembly ◽  
Large Scale ◽  
Three Dimensional ◽  
Random Access ◽  
Scale Production ◽  
Digital Information ◽  
Dna Nanostructures ◽  
Large Scale Production

AbstractThree-dimensional (3D) DNA nanostructures built via DNA self-assembly have established recent applications in multiplexed biosensing and storing digital information. However, a key challenge is that 3D DNA structures are not easily copied which is of vital importance for their large-scale production and for access to desired molecules by target-specific amplification. Here, we build 3D DNA structural barcodes and demonstrate the copying and random access of the barcodes from a library of molecules using a modified polymerase chain reaction (PCR). The 3D barcodes were assembled by annealing a single-stranded DNA scaffold with complementary short oligonucleotides containing 3D protrusions at defined locations. DNA nicks in these structures are ligated to facilitate barcode copying using PCR. To randomly access a target from a library of barcodes, we employ a non-complementary end in the DNA construct that serves as a barcode-specific primer template. Readout of the 3D DNA structural barcodes was performed with nanopore measurements. Our study provides a roadmap for convenient production of large quantities of self-assembled 3D DNA nanostructures. In addition, this strategy offers access to specific targets, a crucial capability for multiplexed single-molecule sensing and for DNA data storage.

scholarly journals Super-Resolution Imaging by Dual Iterative Structured Illumination Microscopy

2021 ◽  
Author(s):  
Anna Loeschberger ◽  
Yauheni Novikau ◽  
Ralf Netz ◽  
Marie-Christin Spindler ◽  
Ricardo Benavente ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Brain Slices ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Living Cells ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Spatiotemporal Resolution ◽  
Coated Pits ◽  
Resolution Imaging

Three-dimensional (3D) multicolor super-resolution imaging in the 50-100 nm range in fixed and living cells remains challenging. We extend the resolution of structured illumination microscopy (SIM) by an improved nonlinear iterative reconstruction algorithm that enables 3D multicolor imaging with improved spatiotemporal resolution at low illumination intensities. We demonstrate the performance of dual iterative SIM (diSIM) imaging cellular structures in fixed cells including synaptonemal complexes, clathrin coated pits and the actin cytoskeleton with lateral resolutions of 60-100 nm with standard fluorophores. Furthermore, we visualize dendritic spines in 70 micrometer thick brain slices with an axial resolution < 200 nm. Finally, we image dynamics of the endoplasmatic reticulum and microtubules in living cells with up to 255 frames/s.

scholarly journals Three dimensional HiLo-based structured illumination for a digital scanned laser sheet microscopy (DSLM) in thick tissue imaging

2013 ◽  
Author(s):  
Dipanjan Bhattacharya ◽  
Vijay Raj Singh ◽  
Chen Zhi ◽  
Peter T. C. So ◽  
Paul Matsudaira ◽  
...  
Keyword(s):  
Laser Sheet ◽  
Three Dimensional ◽  
Tissue Imaging ◽  
Structured Illumination
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