scholarly journals 3D-printed Planktonic Observational Setup and Analysis Pipeline TrackmateTaxis

2020 ◽  
Author(s):  
Clemens C. Döring ◽  
Harald Hausen

AbstractPlanktonic organisms are a cornerstone of marine ecosystems. They vary significantly in size and have a repertoire of behaviors to aid them to survive and navigate their three-dimensional environment. One of the most important cues is light. A variety of setups were used to study the swimming behavior of specific organisms, but broader and comparative investigations need more versatile solutions. With the help of 3D printing, we designed and constructed a modular and flexible behavioral observation setup that enables recordings of animals down to 50μm or up to a few centimeters. A video analysis pipeline using ImageJ and python allows a quick, automated, and robust tracking solution, capable of processing many videos automatically. A modular light path allows the addition of filters or use of pulse width modulation to equalize photon emission of LEDs or additional LEDs to mix different wavelengths. Optionally, a spectrometer can be installed to enable live monitoring of a stimulus. We tested the setup with two phototactic marine planktonic larvae. First, we investigated the spectral sensitivity of the 7-day old larvae of the polychaete Malacoceros fuliginosus and second, the behavior of the 200μm spherical bryozoan coronated larvae of Tricellaria inopinata to ultraviolet light coming from the bottom of the vessel. The setup and pipeline were able to record and analyze hundreds of animals simultaneously. We present an inexpensive, modular, and flexible setup to study planktonic behavior of a variety of sizes.

2012 ◽  
Vol 51 (19) ◽  
pp. 4477 ◽  
Author(s):  
Chao Zuo ◽  
Qian Chen ◽  
Shijie Feng ◽  
Fangxiaoyu Feng ◽  
Guohua Gu ◽  
...  

2013 ◽  
Vol 392 ◽  
pp. 501-506
Author(s):  
S. Rajagopal ◽  
Sheba Charles ◽  
Priyanka Vedula ◽  
C. Bharatiraja ◽  
S.S. Dash

In this paper includes an approach to implement Three Dimensional Space Vector Pulse width Modulation (3D-SVPWM) for multilevel inverter/Converter. The Proposed 3D-SVM scheme finds the reference vector by using simple mathematical equations for selecting switching states without redundant switching vectors. Moreover, the proposed PWM strategy satisfies the constraint that the output voltage vector should be only changed by one switching action. Not requiring extra hardware, the NPC inverter with the proposed PWM results are remarkable. The proposed algorithm is simulated by MATLAB and Implemented by FPGA-DSP Processor.


2010 ◽  
Vol 35 (21) ◽  
pp. 3682 ◽  
Author(s):  
Gastón A. Ayubi ◽  
Jaime A. Ayubi ◽  
J. Matías Di Martino ◽  
José A. Ferrari

2019 ◽  
Vol 9 (23) ◽  
pp. 5087 ◽  
Author(s):  
Y. A. Garces-Gomez ◽  
Fredy E. Hoyos ◽  
John E. Candelo-Becerra

The unified power quality conditioners (UPQCs) are useful to correct distortions in voltage and current waveforms in case of problems related to harmonics, flicker, and power factor. The dual topology of the UPQC allows a loop control in the inverters less demanding in terms of switching functions; however, the control techniques proposed in the literature have some disadvantages as large computation time and some calculation delays. Therefore, this paper presents the application of a classic discrete-time control model for a three-dimensional space vector pulse width modulation (3D-SVPWM) to be used with the dual UPQC, obtaining a fixed commutation frequency and a low computation cost. The results show that the applied method helps to reduce the harmonics in the source, voltage sags and load current and improve the power factor of the electrical circuit tested. Furthermore, the high frequency created by the switching of elements in the UPQC is filtered by the impedance of the power source and it does not represent a problem for the circuit. Due to the simplicity of the model, simulations demonstrate that the application of this classical control technique is enough to achieve good results to compensate harmonics and power factor with the dual UPQC. The development of the controllers is carried out by discretization of the transfer functions of the control in continuous time and their application with the three-dimensional space vector pulse width modulation (3D-SVPWM) technique.


2017 ◽  
Vol 56 (18) ◽  
pp. 5198 ◽  
Author(s):  
Adriana Silva ◽  
Jorge L. Flores ◽  
Antonio Muñoz ◽  
Gastón A. Ayubi ◽  
José A. Ferrari

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