Tinnitus is a prevalent untreatable audiological disorder, charaterized by the perception of phantom sound. Despite longstanding research with animals, its underlying pathophysiology remains poorly understood. The obstacle in progressing in the field may lie in both the current species of choice and the available measurements of tinnitus for animals. To provide fresh impetus, we developed a novel tinnitus-verification technique applicable to rhesus monkeys. Tinnitus was induced via salicylate administration in two rhesus monkeys, and was confirmed by applying a specific eye-blinking procedure: Blinks, as monitored using EMG, were triggered via puffs of air towards the cheek, and their modulation was studied as a function of preceding tones with various frequency and intensity conditions. The advantage in using a tactile reflex-inducing stimulus lies in its non-auditory modality, bypassing potential confounding factors of hearing loss and hyperacusis. Interference effects on the blink modulation pattern was interpreted as tinnitus, and the interfering frequency of the preceding interfering tone as tinnitus frequency. A cross-validation in a sample of tinnitus patients revealed interfering effects of the preceding tone at the specific frequency range corresponding to their own tinnitus frequency, as independently determined by audiologists. This interference effect increased as a function of their individual tinnitus loudness. In conclusion, the present work demonstrates a considerable transferability of our newly established tinnitus-verification technique from non-human primates to human tinnitus patients.