scholarly journals A lateral flow test detecting SARS-CoV-2 neutralizing antibodies

Author(s):  
Nan Zhang ◽  
Shuo Chen ◽  
Jin V. Wu ◽  
Xinhai Yang ◽  
Jianfu J. Wang

AbstractIt is of critical importance for COVID-19 survivors, vaccine recipients, and public to know whether they have developed neutralizing antibodies or immunity. Here, we describe a 15 minutes lateral flow test for rapid detection of neutralizing antibodies against SARS-CoV-2. All other currently available neutralization tests require hours or days to complete and have to be performed in a well-equipped laboratory. This lateral flow test is the first of its kind and will serve as a convenient diagnostic tool in management of COVID-19 disease.

The Analyst ◽  
2020 ◽  
Vol 145 (10) ◽  
pp. 3490-3494 ◽  
Author(s):  
Elena Costa ◽  
Estela Climent ◽  
Sandra Ast ◽  
Michael G. Weller ◽  
John Canning ◽  
...  

The employment of type-I pyrethroids for airplane disinfection in recent years underlines the necessity to develop sensing schemes for the rapid detection of these pesticides directly at the point-of-use.


2021 ◽  
Author(s):  
Guo-Qiang Zhang ◽  
Zhiyuan Gao ◽  
Jingtian Zhang ◽  
Heqi Gao ◽  
Ryan T. K. Kwok ◽  
...  

2007 ◽  
Vol 55 (6) ◽  
pp. 2072-2078 ◽  
Author(s):  
Xiliang Wang ◽  
Kui Li ◽  
Deshi Shi ◽  
Ning Xiong ◽  
Xiue Jin ◽  
...  

2018 ◽  
Vol 286 ◽  
pp. 6-14 ◽  
Author(s):  
Wanwisa Poonlapdecha ◽  
Yortyot Seetang-Nun ◽  
Wijit Wonglumsom ◽  
Kooranee Tuitemwong ◽  
Larry E. Erickson ◽  
...  

2018 ◽  
Vol 1008 ◽  
pp. 1-7 ◽  
Author(s):  
Ruili Wu ◽  
Shuai Zhou ◽  
Ting Chen ◽  
Jinjie Li ◽  
Huaibin Shen ◽  
...  

2021 ◽  
Vol 24 (4) ◽  
pp. 497-507
Author(s):  
R Soliman ◽  
M. M. Magdy ◽  
A. Samir ◽  
Y. A. Abdalla ◽  
R. H. Sayed

In the present work a lateral flow immunochromatographic test (LFT) for rapid detection of Clostri­dium perfringens toxins types, alpha (α), beta (β) and epsilon (ε) in clinical samples was developed. C. perfringens toxins were prepared, purified and inactivated with 0.2% formalin. Polyclonal antibodies specific to C. perfringens toxins types α, β and ε toxoids were prepared in rabbits and guinea pigs. The toxoid specific polyclonal antibodies prepared in rabbits were labelled with gold chloride nanoparticles. The prepared toxin specific rabbit and guinea pigs antibodies and goat anti-rabbit antibodies were utilised in development of a lateral flow immunochromatographic test and the latter - evaluated for detection of C. perfringens α, β and ε toxins in clinical samples. The sensitivity and specificity and accuracy of the developed LFT were determined by comparison with a commercially available ELISA used for detection of these toxins. The prepared LFT was capable to detect C. perfringens α, β and ε toxins in quantities of 2 μg/ml, 250 ng/ml and 60 ng/ml, respectively. One hundred poultry suspected faecal samples was examined both with the prepared LFT and commercial ELISA to test the validity of developed LFT. The sensitivity, specificity and accuracy of the LFT for detection of C. perfringens toxins were 81%, 95.2% and 90%, respectively, for α toxin, 76.6%, 98.5% and 72%, respectively, for β toxin and 66.6%, 98.8% and 95%, respectively, for ε toxin.


2022 ◽  
pp. 100740
Author(s):  
Guo-Qiang Zhang ◽  
Zhiyuan Gao ◽  
Jingtian Zhang ◽  
Hanlin Ou ◽  
Heqi Gao ◽  
...  

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