scholarly journals Characterization of chitin deacetylase genes in the Diaphorina citri genome

2020 ◽  
Author(s):  
Sherry Miller ◽  
Teresa D. Shippy ◽  
Blessy Tamayo ◽  
Prashant S Hosmani ◽  
Mirella Flores-Gonzalez ◽  
...  
Keyword(s):  
Diaphorina Citri ◽  
Chitin Deacetylase ◽  
Citrus Greening Disease ◽  
Collaborative Community ◽  
Liberibacter Asiaticus ◽  
Annotation Project ◽  
Greening Disease ◽  
Long Fibers ◽  
Community Annotation

AbstractChitin deacetylases (CDAs) are one of the least understood components of insect chitin metabolism. The partial deacetylation of chitin polymers appears to be important for the proper formation of higher order chitin structures, such as long fibers and bundles, that contribute to the integrity of the insect exoskeleton and other structures. Some CDAs may also play a role in bacterial defense. Here we report the characterization of four CDA genes from the Asian citrus psyllid, Diaphorina citri, laying the groundwork for future study of these genes. D. citri is the vector for Candidatus Liberibacter asiaticus (CLas), which is responsible for the global outbreak of Huanglongbing (citrus greening) disease. The manual annotation was done as part of a collaborative community annotation project (https://citrusgreening.org/annotation/index).

scholarly journals Asian citrus psyllid adults inoculate huanglongbing bacterium more efficiently than nymphs when this bacterium is acquired by early instar nymphs

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
El-Desouky Ammar ◽  
Justin George ◽  
Kasie Sturgeon ◽  
Lukasz L. Stelinski ◽  
Robert G. Shatters
Keyword(s):  
Latent Period ◽  
Salivary Glands ◽  
Emerging Adults ◽  
Citrus Greening ◽  
Asian Citrus Psyllid ◽  
Diaphorina Citri ◽  
Citrus Greening Disease ◽  
Early Instar ◽  
Liberibacter Asiaticus ◽  
Greening Disease

Abstract The Asian citrus psyllid (Diaphorina citri) transmits the bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), which causes huanglongbing (citrus greening) disease, in a circulative-propagative manner. We compared CLas inoculation efficiency of D. citri nymphs and adults into healthy (uninfected) citron leaves when both vector stages were reared from eggs on infected plants. The proportion of CLas-positive leaves was 2.5% for nymphs and 36.3% for adults. CLas acquisition by early instar nymphs followed by dissections of adults and 4th instar nymphs revealed that CLas bacterium had moved into the head-thorax section (containing the salivary glands) in 26.7–30.0% of nymphs and 37–45% of adults. Mean Ct values in these sections were 31.6–32.9 and 26.8–27.0 for nymphs and adults, respectively. Therefore, CLas incidence and titer were higher in the head-thorax of adults than in nymphs. Our results suggest that following acquisition of CLas by early instar D. citri nymphs, emerging adults inoculate the bacteria into citrus more efficiently than nymphs because adults are afforded a longer latent period necessary for multiplication and/or translocation of CLas into the salivary glands of the vector. We propose that CLas uses D. citri nymphs mainly for pathogen acquisition and multiplication, and their adults mainly for pathogen inoculation and spread.

Characterization of the heavy metal binding properties of periplasmic metal uptake protein CLas-ZnuA2

Metallomics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 280-289 ◽  
Author(s):  
Pranav Kumar ◽  
Vikram Dalal ◽  
Nidhi Sharma ◽  
Sunil Kokane ◽  
Dilip Kumar Ghosh ◽  
...  
Keyword(s):  
Metal Binding ◽  
Metal Uptake ◽  
Citrus Greening ◽  
Negative Bacterium ◽  
Binding Properties ◽  
Citrus Greening Disease ◽  
Heavy Metal Binding ◽  
Liberibacter Asiaticus ◽  
Greening Disease

Candidatus Liberibacter asiaticus (CLas), a phloem-limited unculturable Gram-negative bacterium, causes citrus greening disease.

scholarly journals In silico characterization of chitin deacetylase genes in the Diaphorina citri genome

Gigabyte ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Sherry Miller ◽  
Teresa D. Shippy ◽  
Blessy Tamayo ◽  
Prashant S. Hosmani ◽  
Mirella Flores-Gonzalez ◽  
...  
Keyword(s):  
Future Study ◽  
In Silico ◽  
Higher Order ◽  
Asian Citrus Psyllid ◽  
Manual Annotation ◽  
Diaphorina Citri ◽  
Chitin Deacetylase ◽  
In Silico Characterization ◽  
Long Fibers

Chitin deacetylases (CDAs) are one of the least understood components of insect chitin metabolism. The partial deacetylation of chitin polymers appears to be important for the proper formation of higher order chitin structures, such as long fibers and bundles, which contribute to the integrity of the insect exoskeleton and other structures. Some CDAs may also be involved in bacterial defense. Here, we report the manual annotation of four CDA genes from the Asian citrus psyllid, Diaphorina citri, laying the groundwork for future study of these genes.

scholarly journals Candidatus Liberibacter Asiaticus Manipulates the Expression of Vitellogenin, Cytoskeleton, and Endocytotic Pathway-Related Genes to Become Circulative in Its Vector, Diaphorina citri (Hemiptera: Psyllidae)

2020 ◽  
Author(s):  
Damini Jaiswal ◽  
V. Kavi Sidharthan ◽  
Susheel Kumar Sharma ◽  
Richa Rai ◽  
Nandlal Choudhary ◽  
...  
Keyword(s):  
Differential Regulation ◽  
Citrus Greening ◽  
Insect Vector ◽  
Diaphorina Citri ◽  
Citrus Greening Disease ◽  
Physiological Processes ◽  
Citrus Production ◽  
Liberibacter Asiaticus ◽  
Greening Disease ◽  
Cellular Components

Citrus greening disease or huanglongbing (HLB) caused by Candidatus Liberibacter asiaticus (CLas) limits the citrus production worldwide. CLas is transmitted by the Asian citrus psyllid (ACP), Diaphorina citri (Hemiptera: Psyllidae) in a persistent-propagative manner. Application of insecticides to manage the psyllid vectors and disease is the most common practice. Understanding the molecular interaction between CLas and ACP and interrupting the interrelationship can provide an alternative to insecticides for managing citrus greening disease. Transcriptome analysis of ACP in response to CLas showed differential expression of 3911 genes (2196 up-regulated, and 1715 down-regulated) including the key genes of ACP involved in cytoskeleton synthesis and nutrition-related proteins. Majority of the differentially expressed genes were categorized under molecular function followed by cellular components and biological processes. KEGG pathway analysis showed differential regulation of carbohydrate, nucleotide and energy metabolic pathways, the endocytotic pathway and the defense-related pathways. Differential regulation of genes associated with the key pathways might favors CLas to become systemic and propagate in its insect vector. The study provides an understanding of genes involved in circulation of CLas in ACP. The candidate genes involved in key physiological processes and CLas transmission by ACP would be potential targets for sustainable management of ACP and CLas.

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