scholarly journals Characterization of the nanomechanical properties of the fission yeast (Schizosaccharomyces pombe) cell surface by atomic force microscopy

2021 ◽  
Author(s):  
Ellie Gibbs ◽  
Justine Hsu ◽  
Kathryn Barth ◽  
John W. Goss
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Cell Surface ◽  
Fission Yeast ◽  
Surface Topography ◽  
Polarized Growth ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force

ABSTRACTVariations in cell wall composition and biomechanical properties can contribute to the cellular plasticity required during complex processes such as polarized growth and elongation in microbial cells. This study utilizes atomic force microscopy (AFM) to map the cell surface topography of fission yeast, Schizosaccharomyces pombe, at regions of active polarized growth and to characterize the biophysical properties within these regions under physiological, hydrated conditions. High-resolution images acquired from AFM topographic scanning reveal decreased surface roughness at actively growing cell poles. Force extension curves acquired by nanoindentation probing with AFM cantilever tips under low applied force revealed increased cell wall elasticity and decreased cellular stiffness (cellular spring constant) at cell poles (17 ± 4 mN/m) relative to the main body of the cell that is not undergoing growth and expansion (44 ± 10 mN/m). These findings suggest that the increased elasticity and decreased stiffness at regions undergoing polarized growth at fission yeast cell poles provide the plasticity necessary for cellular extension. This is the first direct biophysical characterization of the S. pombe cell surface by AFM, and it provides a foundation for future investigation of how the surface topography and local nanomechanical properties vary during different cellular processes.

scholarly journals Structural and Topological Differences between a Glycopeptide-Intermediate Clinical Strain and Glycopeptide-Susceptible Strains of Staphylococcus aureus Revealed by Atomic Force Microscopy

2000 ◽  
Vol 44 (12) ◽  
pp. 3456-3460 ◽  
Author(s):  
Susan Boyle-Vavra ◽  
Jongin Hahm ◽  
S. J. Sibener ◽  
Robert S. Daum
Keyword(s):  
Staphylococcus Aureus ◽  
Atomic Force Microscopy ◽  
Cell Surface ◽  
Surface Topography ◽  
Clinical Strain ◽  
Force Microscopy ◽  
Atomic Force

ABSTRACT Novel cell surface topography was revealed on cocci from a glycopeptide-intermediate Staphylococcus aureus (GISA) clinical strain by using atomic force microscopy. The GISA isolate and its revertant had two parallel circumferential surface rings. One equatorial surface ring was observed in control strains. In vancomycin-susceptible strains, additional rings were formed in the presence of vancomycin. Ring depth measurements also revealed striking differences between the GISA strain and susceptible strains grown with or without vancomycin.

Measurement of nanomechanical properties of DNA molecules by PeakForce atomic force microscopy based on DNA origami

Nanoscale ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 4707-4711 ◽  
Author(s):  
Lin Li ◽  
Ping Zhang ◽  
Jiang Li ◽  
Ying Wang ◽  
Yuhui Wei ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Origami ◽  
Dna Molecules ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force ◽  
Dna Strands

Characterization of the stiffness of thin DNA strands remains difficult.

scholarly journals Evidence for a Role for the Plasma Membrane in the Nanomechanical Properties of the Cell Wall as Revealed by an Atomic Force Microscopy Study of the Response of Saccharomyces cerevisiae to Ethanol Stress

2016 ◽  
Vol 82 (15) ◽  
pp. 4789-4801 ◽  
Author(s):  
Marion Schiavone ◽  
Cécile Formosa-Dague ◽  
Carolina Elsztein ◽  
Marie-Ange Teste ◽  
Helene Martin-Yken ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Cell Membrane ◽  
Yeast Cells ◽  
Ethanol Stress ◽  
Wild Type ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force

ABSTRACTA wealth of biochemical and molecular data have been reported regarding ethanol toxicity in the yeastSaccharomyces cerevisiae. However, direct physical data on the effects of ethanol stress on yeast cells are almost nonexistent. This lack of information can now be addressed by using atomic force microscopy (AFM) technology. In this report, we show that the stiffness of glucose-grown yeast cells challenged with 9% (vol/vol) ethanol for 5 h was dramatically reduced, as shown by a 5-fold drop of Young's modulus. Quite unexpectedly, a mutant deficient in the Msn2/Msn4 transcription factor, which is known to mediate the ethanol stress response, exhibited a low level of stiffness similar to that of ethanol-treated wild-type cells. Reciprocally, the stiffness of yeast cells overexpressingMSN2was about 35% higher than that of the wild type but was nevertheless reduced 3- to 4-fold upon exposure to ethanol. Based on these and other data presented herein, we postulated that the effect of ethanol on cell stiffness may not be mediated through Msn2/Msn4, even though this transcription factor appears to be a determinant in the nanomechanical properties of the cell wall. On the other hand, we found that as with ethanol, the treatment of yeast with the antifungal amphotericin B caused a significant reduction of cell wall stiffness. Since both this drug and ethanol are known to alter, albeit by different means, the fluidity and structure of the plasma membrane, these data led to the proposition that the cell membrane contributes to the biophysical properties of yeast cells.IMPORTANCEEthanol is the main product of yeast fermentation but is also a toxic compound for this process. Understanding the mechanism of this toxicity is of great importance for industrial applications. While most research has focused on genomic studies of ethanol tolerance, we investigated the effects of ethanol at the biophysical level and found that ethanol causes a strong reduction of the cell wall rigidity (or stiffness). We ascribed this effect to the action of ethanol perturbing the cell membrane integrity and hence proposed that the cell membrane contributes to the cell wall nanomechanical properties.

Stereometric characterization of Dinizia excelsa Ducke wood from Amazon rainforest using atomic force microscopy

2021 ◽  
Author(s):  
Willian Silva Conceição ◽  
Ştefan Ţălu ◽  
Robert Saraiva Matos ◽  
Glenda Quaresma Ramos ◽  
Fidel Guereiro Zayas ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Amazon Rainforest ◽  
Force Microscopy ◽  
Atomic Force ◽  
Dinizia Excelsa
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